lncRNA PCAT14对甲状腺癌细胞侵袭转移的影响

Effects of lncRNA PCAT14 on Invasion and Metastasis of Thyroid Cancer Cells

摘要: 目的：探讨lncRNA PCAT14对甲状腺癌细胞增殖和侵袭的影响及其分子机制。方法：生物信息学方法预测lncRNA PCAT14表达与肾癌患者临床分期的相关性。选择TPC-1甲状腺癌细胞系转染，分别将PCAT14质粒(实验组)或空载对照质粒(对照组)转至甲状腺癌细胞中。使用qPCR检测PCAT14质粒转染效率。细胞计数实验及克隆形成实验检测转染后细胞增殖活性，Transwell侵袭实验检测转染后甲状腺细胞的侵袭能力。Western blot检测EMT相关标记物的蛋白表达水平。生物信息学方法预测lncRNA PCAT14可能结合的蛋白。结果：TCGA数据库显示，甲状腺癌组织lncRNA PCAT14相对表达水平显著低于癌旁正常组织(P < 0.05)。lncRNA PCAT14在甲状腺癌晚期患者肿瘤组织中相对表达量更低(P < 0.01)。与对照组比较，实验组lncRNA PCAT14相对表达水平显著升高(P < 0.05)，细胞增殖活性(P < 0.05)和细胞的侵袭能力显著降低(P < 0.01)。同时，实验组lncRNA PCAT14过表达抑制了EMT相关蛋白的表达。lncRNA PCAT14可能与DBX2结合发挥生物学功能。结论：lncRNA PCAT14在甲状腺癌组织中低表达，与肾癌患者临床分期相关。过表达lncRNA PCAT14可明显抑制肾癌细胞增殖和侵袭，其分子机制可能为lncRNA PCAT14与DBX2结合发挥生物学功能。

Abstract: Objective: To investigate the effect of lncRNA PCAT14 on the invasion and metastasis of thyroid cancer cells and its molecular mechanism. Methods: Bioinformatics methods predict the correlation between the expression of lncRNA PCAT14 and the clinical stage of renal cancer patients. Select TPC-1 thyroid cancer cell line for transfection, and transfer PCAT14 plasmid (experimental group) or empty control plasmid (control group) to thyroid cancer cells respectively. QPCR was used to detect the transfection efficiency of PCAT14 plasmid. Cell counting experiment and clone formation experiment were used to detect cell proliferation activity after transfection, and Transwell invasion experiment was used to detect the invasion ability of thyroid cells after transfection. Western blot was used to detect the protein expression level of EMT-related markers. Bioinformatics methods predict the protein that lncRNA PCAT14 may bind. Results: The TCGA database showed that the relative expression level of lncRNA PCAT14 in thyroid cancer tissues was significantly lower than that in normal tissues adjacent to cancer (P < 0.05). The relative expression of lncRNA PCAT14 is lower in the cancer tissues of patients with advanced thyroid cancer (P < 0.01). Compared with the control group, the relative expression level of lncRNA PCAT14 of the experimental group was significantly increased (P < 0.05), and the cell proliferation activity (P < 0.05) and cell invasion ability were significantly reduced (P < 0.01). At the same time, the lncRNA PCAT14 overexpression in the experimental group inhibited the expression of EMT-related proteins. lncRNA PCAT14 may combine with DBX2 to exert biological functions. Conclusion: lncRNA PCAT14 is lowly expressed in thyroid cancer tissues and is related to the clinical stage of renal cancer patients. Overexpression of lncRNA PCAT14 can significantly inhibit the proliferation and invasion of renal cancer cells. The molecular mechanism may be that lncRNA PCAT14 combine with DBX2 to exert biological functions.