乙醇/磷酸氢二钾双水相体系萃取水样中萘普生和尼莫地平的研究

Study on Extraction of Naproxen and Nimodipine from Water Sample by Ethanol/Dipotassium Hydrogen Phosphate Dual-Aqueous Phase System

摘要: 建立乙醇/磷酸氢二钾双水相萃取–高效液相色谱法测定水样中萘普生和尼莫地平的分析方法，考察了不同的超声时间、超声功率、KH2PO4质量分数和乙醇质量分数对萃取率的影响，通过响应曲面试验设计进一步优化确定最佳萃取条件，结果得到乙醇/磷酸氢二钾双水相体系萃取水样中萘普生和尼莫地平的最佳条件为：在超声功率200 W，离心转速5000 r/min，离心10 min时间下，乙醇质量分数为39%，KH2PO4质量分数为18%，超声时间15 min，萘普生的萃取率可达94.73%，尼莫地平的萃取率可达88.57%。该方法简便、快速、准确，能满足萘普生和尼莫地平的萃取要求。

Abstract: Establishing an ethanol/dipotassium hydrogen phosphate dual-aqueous phase system to extrac-tion—high-efficiency liquid chromatography determines the Naproxen and Nimodipine in water samples’ analytical method. The effects of different ultrasonic times, ultrasonic power, KH2PO4 mass fraction and ethanol mass fraction on the extraction efficiency were investigated. The optimal extraction conditions were further optimized by response surface test design. The results showed that the best conditions of ethanol/dipotassium hydrogen phosphate dual-aqueous phase system to extraction the Naproxen and Nimodipine in water samples is: Under the ultrasonic power of 200 W, centrifugation speed of 5000 r/min, centrifugation time of 10 min, mass fraction of ethanol is 39%, mass fraction of KH2PO4 is 18%, ultrasonic time of 15 min, the extraction rate of Naproxen and Ni-modipine can reach 94.73% and 88.57%. The method is simple, fast and accurate, and can meet the extraction requirements of naproxen and nimodipine.