TiO2微球的制备及应用于外泌体的吸附
Preparation and Application of TiO2 Microspheres for Separation of Exosome
DOI: 10.12677/MS.2021.117095, PDF,    国家自然科学基金支持
作者: 陈 竞*, 林建伟*, 何玉彬, 马福新, 韩志钟#：福建医科大学药学院，福建 福州；宋泽良：福建医科大学临床医学部，福建 福州
关键词: TiO2微球；外泌体；吸附；Titanium Dioxide Microspheres； Exosome； Adsorption
摘要: 外泌体具有多种生物学功能，对疾病的治疗和诊断具有重要的意义，而获得高纯度的外泌体对后续的研究至关重要。本实验采用溶剂热法，以钛酸四异丙酯作为前驱物，EDTA作为包裹剂，优化实验条件，制备出粒径为1~3 μm、分散性良好、表面光滑的TiO2微球。利用TiO2微球表面Lewis酸位点实现对外泌体的特性吸附及纯化，并采用扫描电镜(SEM)、透射电镜(TEM)进行表征观察。结果表明，TiO2微球能够高效地吸附外泌体，使用10%氨水可以快速将外泌体从TiO2微球表面洗脱下来且不破坏外泌体的囊泡结构。
Abstract: Exosome has a variety of biological functions and is of great significance for the treatment and di-agnosis of diseases. And it is very important to obtain high purity exosome for subsequent research. In this experiment, TiO2 microspheres were prepared by hot solvent method with tetrai-sopropyltitanate as precursor and EDTA as precipitant. Optimizing experimental conditions, TiO2 microspheres with particle size of 1~3 μm, good dispersion and smooth surface were successfully prepared. Exosomes were adsorbed and purified by Lewis acid sites on the surface of TiO2 micro-spheres, and characterized by scanning electron microscopy (SEM) and transmission electron mi-croscopy (TEM). The results show that TiO2 microspheres could adsorb efficiently exosome. 10% ammonia could quickly elute exosome from the surface of TiO2 microspheres without destroying the vesicle structure of exocrine.
文章引用：陈竞, 林建伟, 何玉彬, 马福新, 宋泽良, 韩志钟. TiO2微球的制备及应用于外泌体的吸附[J]. 材料科学, 2021, 11(7): 824-834. https://doi.org/10.12677/MS.2021.117095

参考文献

[1] Hessvik, N.P. and Llorente, A. (2018) Current Knowledge on Exosome Biogenesis and Release. Cellular and Molecular Life Sciences, 75, 193-208. [Google Scholar] [CrossRef] [PubMed]
[2] Subra, C., Grand, D., Laulagnier, K., Stella, A., Lambeau, G., Paillasse, M., et al. (2010) Exosomes Account for Vesicle-Mediated Transcellular Transport of Activatable Phospholipases and Prostaglandins. Journal of Lipid Research, 51, 2105-2120. [Google Scholar] [CrossRef] 
[3] Subra, C., Laulagnier, K., Perret, B. and Record, M. (2007) Exosome Lipidomics Unravels Lipid Sorting at the Level of Multivesicular Bodies. Biochimie, 89, 205-212. [Google Scholar] [CrossRef] [PubMed]
[4] Batista, B.S., Eng, W.S., Pilobello, K.T., Hendricks-Muñoz, K.D. and Mahal, L.K. (2011) Identification of a Conserved Glycan Signature for Microvesicles. Journal of Proteome Research, 10, 4624-4633. [Google Scholar] [CrossRef] [PubMed]
[5] Sun, D., Zhuang, X., Zhang, S., Deng, Z.-B., Grizzle, W., Miller, D., et al. (2013) Exosomes Are Endogenous Nanoparticles That Can Deliver Biological Information between Cells. Advanced Drug Delivery Reviews, 65, 342-347. [Google Scholar] [CrossRef] [PubMed]
[6] Lobb, R.J., Becker, M., Wen, S., Wong, C.S.F., Wiegmans, A.P., Leimgruber, A., et al. (2015) Optimized Exosome Isolation Protocol for Cell Culture Supernatant and Human Plasma. Journal of Extracellular Vesicles, 4, Article No. 27031. [Google Scholar] [CrossRef] [PubMed]
[7] 胡国文, 李青, 牛鑫, 胡斌, 刘鹃, 沈晓黎, 等. 旋转超滤: 一种提取细胞外泌体的新方法[J]. 第二军医大学学报, 2014(35): 598-602.
[8] Zhang, X., Yuan, X., Shi, H., Wu, L., Qian, H. and Xu, W. (2015) Exosomes in Cancer: Small Particle, Big Player. Journal of Hematology &Oncology, 8, Article No. 83. [Google Scholar] [CrossRef] [PubMed]
[9] Chen, L., Chen, R. and Brigstock, D. (2014) Microrna Profiling of Circulating Exosomes during Experimental Liver Fibrosis: 430. Hepatology, 60, Article No. 412A.
[10] An, H.R., Hong, Y.C., Kim, H., Huh, J.Y., Park, E.C., Park, S.Y., et al. (2018) Studies on Mass Production and Highly Solar Light Photocatalytic Properties of Gray Hydrogenated-TiO2 Sphere Photocatalysts. Journal of Hazardous Materials, 358, 222-233. [Google Scholar] [CrossRef] [PubMed]
[11] Nguyen-Le, M.T. and Lee, B.K. (2017) Effective Photodegradation of Dyes Using In-Situ N-Ti3+ Co-Doped Porous Titanate-TiO2 Rod-Like Heterojunctions. Catalysis Today, 297, 228-236. [Google Scholar] [CrossRef] 
[12] 万婷婷. 二氧化钛复合纳米纤维的制备及其在染料敏化太阳能电池的应用研究[D]: [硕士学位论文]. 北京: 北京化工大学, 2018.
[13] Zhang, X., Zuo, G., Lu, X., Tang, C., Cao, S. and Yu, M. (2017) Anatase TiO2 Sheet-Assisted Synthesis of Ti3+ Self-Doped Mixed Phase TiO2Sheet with Superior Visible-light Photocatalytic Performance: Roles of Anatase TiO2 Sheet. Journal of Colloid and Interface Science, 490, 774-782. [Google Scholar] [CrossRef] [PubMed]
[14] 魏秋实. 二氧化钛的表面改性和复合改性及其在水处理中的应用研究[D]: [硕士学位论文]. 广州: 华南理工大学, 2018.
[15] Thingholm, T.E. and Larsen, M.R. (2016) Phospho-Proteomics. In: von Stechow, L., Ed., Phospho-Proteomics, Vol. 1355, Springer, New York, 135-146. [Google Scholar] [CrossRef] [PubMed]
[16] Leitner, A. (2010) Phosphopeptide Enrichment Using Metal Oxide Affinity Chromatography. TrAC Trends in Analytical Chemistry, 29, 177-185. [Google Scholar] [CrossRef] 
[17] Gao, F., Jiao, F., Xia, C., Zhao, Y., Ying, W., Xie, Y., et al. (2019) A novel Strategy for Facile Serum Exosome Isolation Based on Specific Interactions between Phospholipid Bilayers and TiO2. Chemical Science, 10, 1579-1588. [Google Scholar] [CrossRef] 
[18] Yu, X., Shang, L., Wang, D., An, L., Li, Z., Liu, J., et al. (2018) Plasmon-Resonance-Enhanced Visible-Light Photocatalytic Activity of Ag Quantum Dots/TiO2Microspheres for Methyl Orange Degradation. Solid State Sciences, 80, 1-5. [Google Scholar] [CrossRef] 
[19] 刘波, 岳林海, 李国华, 宋旭春, 陈卫祥, 徐铸德. 纳米二氧化钛粉体表面的吸附特征及其对粉体分散性的影响[J]. 浙江大学学报(理学版), 2004, 31(1): 79-83.
[20] 景晓辉, 卑圣金, 蔡再生, 石健, 丁欣宇. 锐钛矿型TiO2的制备与光催化性能研究[J]. 针织工业, 2008(10): 61-65.
[21] Thingholm, T.E., Jørgensen, T.J.D., Jensen, O.N. and Larsen, M.R. (2006) Highly Selective Enrichment of Phosphorylated Peptides Using Titanium Dioxide. Nature Protocols, 1, 1929-1935. [Google Scholar] [CrossRef] [PubMed]

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