极端环境胁迫下金精矿生物氧化过程研究进展
Research Progress on Biological Oxidation Process of Gold Concentrate under Extreme Environmental Stress
摘要: 生物氧化已经商业化地应用于从金精矿中提取金，尤其在难处理金精矿方面具有显著优势，从而扩大黄金的储量。可以采用系统生物学手段阐述极端环境下单菌株和多菌株体系的适应性机制，这将有助于为生物氧化建立更具抵抗力的微生物群落，并发挥最大的作用。同时，在生物反应器中进行金精矿的生物氧化是一种高效的冶金方法。由于生物反应器内存在复杂的三相生物氧化过程，有必要建立计算流体动力学(CFD)模型来模拟金精矿的生物氧化过程，通过该模型得到的最佳操作条件可以进一步提高实际生产过程中的各项指标。本文综述了系统生物学应用于浸出微生物适应性机制及对金精矿生物氧化过程建模的研究现状和发展趋势。
Abstract: Bio-oxidation has been commercially applied to extract gold from gold concentrate, and has significant advantages especially when handling refractory gold concentrate, thereby expanding the recoverable gold reserves. The adaptive mechanisms of single- and multi-strain systems under extreme environments can be explained by means of systems biology, which will help to build a more stress-resistant microbial community for bio-oxidation with maximal effectiveness. At the same time, the bio-oxidation of gold concentrate in bioreactors is an efficient metallurgical method. Due to the complex bio-oxidation processes inside bioreactors, it is necessary to establish a computational fluid dynamics (CFD) model to simulate the dissolution process of gold concentrate, and the optimal operating conditions obtained from such models can further improve the indicators in the actual production processes. This article reviews the research status and development trend of systems biology applied to the adaptive mechanisms of leaching microorganisms and the modeling of the gold concentrate dissolution process in bioreactors.
文章引用：李敏, 闻建平. 极端环境胁迫下金精矿生物氧化过程研究进展[J]. 冶金工程, 2021, 8(3): 95-104. https://doi.org/10.12677/MEng.2021.83012

参考文献

[1] Rawlings, D.E., Dew, D. and du Plessis, C. (2003) Biomineralization of Metal-Containing Ores and Concentrates. Trends in Biotechnology, 21, 38-44. [Google Scholar] [CrossRef] 
[2] Kaksonen, A.H., Mudunuru, B.M. and Hackl, R. (2014) The Role of Microorganisms in Gold Processing and Recovery—A Review. Hy-drometallurgy, 142, 70-83. [Google Scholar] [CrossRef] 
[3] Kaksonen, A.H., Boxall, N.J., Gumulya, Y., Khaleque, H.N., Morris, C., Bohu, T., Cheng, K.Y., Usher, K.M. and Lakaniemi, A.-M. (2018) Recent Progress in Biohydrometallurgy and Microbial Characterisation. Hydrometallurgy, 180, 7-25. [Google Scholar] [CrossRef] 
[4] 郝福来. 生物冶金技术的发展及其在黄金行业中的应用现状[J]. 黄金, 2019, 40(5): 55-60.
[5] De Sousa, C.S., Hassan, S.S., Pinto, A.C., Silva, W.M., De Almeida, S.S., De Castro Soares, S., Azevedo, M.S.P., Rocha, C.S., Barh, D. and Azevedo, V. (2018) Chapter 1—Microbial Omics: Ap-plications in Biotechnology. In: Barh, D. and Azevedo, V., Eds., Omics Technologies and Bio-Engineering, Academic Press, Cambridge, 3-20. [Google Scholar] [CrossRef] 
[6] Jerez, C.A. (2008) The Use of Genomics, Proteomics and Other OMICS Technologies for the Global Understanding of Biomining Microorganisms. Hydrometallurgy, 94, 162-169. [Google Scholar] [CrossRef] 
[7] Watling, H. (2016) Microbiological Advances in Biohydrometallurgy. Minerals, 6, 49. [Google Scholar] [CrossRef] 
[8] Shahab, R.L., Brethauer, S., Davey, M.P., Smith, A.G., Vignolini, S., Luterbacher, J.S. and Studer, M.H. (2020) A Heterogeneous Microbial Consortium Producing Short-Chain Fatty Acids from Lignocellulose. Science, 369, Article ID: 1073. [Google Scholar] [CrossRef] [PubMed]
[9] Liu, W., Jacqui-od, S., Brejnrod, A., Russel, J., Burmølle, M. and Sørensen, S.J. (2019) Deciphering Links between Bacterial Interac-tions and Spatial Organization in Multispecies Biofilms. The ISME Journal, 13, 3054-3066. [Google Scholar] [CrossRef] [PubMed]
[10] Natarajan, K.A. (2018) Chapter 6—Bioleaching of Copper and Uranium. In: Natarajan, K.A., Ed., Biotechnology of Metals, Elsevier, Amsterdam, 107-150. [Google Scholar] [CrossRef] 
[11] Mahmoud, A., Cézac, P., Hoadley, A.F.A., Contamine, F. and D'Hugues, P. (2017) A Review of Sulfide Minerals Microbially Assisted Leaching in Stirred Tank Reactors. In-ternational Biodeterioration & Biodegradation, 119, 118-146. [Google Scholar] [CrossRef] 
[12] Zhu, J.Y., Zhang, J.X., Li, Q., Han, T., Hu, Y.H., Liu, X.D., Qin, W.Q., Chai, L.Y. and Qiu, G.Z. (2014) Bioleaching of Heavy Metals from Contaminated Alkaline Sediment by Auto- and Heterotrophic Bacteria in Stirred Tank Reactor. Transactions of Nonferrous Metals Society of China, 24, 2969-2975. [Google Scholar] [CrossRef] 
[13] Hong, J., Silva, R.A., Park, J., Lee, E., Park, J. and Kim, H. (2016) Adaptation of a Mixed Culture of Acidophiles for a Tank Biooxidation of Refractory Gold Concentrates Contain-ing a High Concentration of Arsenic. Journal of Bioscience and Bioengineering, 121, 536-542. [Google Scholar] [CrossRef] [PubMed]
[14] Hu, W.B., Feng, S.S., Tong, Y.J., Zhang, H.L. and Yang, H.L. (2020) Adaptive Defensive Mechanism of Bioleaching Microorganisms under Extremely Environmental Acid Stress: Advances and Perspectives. Biotechnology Advances, 42, Article ID: 107580. [Google Scholar] [CrossRef] [PubMed]
[15] González-Toril, E. and Aguilera, Á. (2019) Chapter 14 - Microbial Ecology in Extreme Acidic Environments: Use of Molecular Tools. In: Das, S. and Dash, H.R., Eds., Microbi-al Diversity in the Genomic Era, Academic Press, Cambridge, 227-238. [Google Scholar] [CrossRef] 
[16] Li, Q. and Sand, W. (2017) Mechanical and Chemical Studies on EPS from Sulfobacillus thermosulfidooxidans: From Planktonic to Biofilm Cells. Colloids and Surfaces B: Biointerfaces, 153, 34-40. [Google Scholar] [CrossRef] [PubMed]
[17] Feng, S., Yang, H. and Wang, W. (2015) System-Level Un-derstanding of the Potential Acid-Tolerance Components of Acidithiobacillus thiooxidans ZJJN-3 Under extreme Acid Stress. Extremophiles, 19, 1029-1039. [Google Scholar] [CrossRef] [PubMed]
[18] Zhang, X., Liu, X.D., Liang, Y.L., Guo, X., Xiao, Y.H., Ma, L.Y., Miao, B., Liu, H.W., Peng, D.L., Huang, W.K., et al. (2017) Adaptive Evolution of Extreme Acidophile Sulfobacillus thermosulfidooxidans Potentially Driven by Horizontal Gene Transfer and Gene Loss. Applied and Environmental Mi-crobiology, 83, 18. [Google Scholar] [CrossRef] 
[19] Christel, S., Herold, M., Bellenberg, S., El Hajjami, M., Buetti-Dinh, A., Pivkin, I.V., Sand, W., Wilmes, P., Poetsch, A. and Dopson, M. (2018) Multi-omics Reveals the Lifestyle of the Acidophilic, Mineral-Oxidizing Model Species Leptospirillum ferriphilum (T). Applied and Environmental Microbiology, 84, 17. [Google Scholar] [CrossRef] 
[20] Baker-Austin, C. and Dopson, M. (2007) Life in Acid: pH Homeo-stasis in Acidophiles. Trends in Microbiology, 15, 165-171. [Google Scholar] [CrossRef] [PubMed]
[21] Dopson, M. and Holmes, D.S. (2014) Metal Resistance in Acidophilic Microorganisms and Its Significance for Biotechnologies. Applied Microbiology and Biotechnology, 98, 8133-8144. [Google Scholar] [CrossRef] [PubMed]
[22] Valdés, J., Cárdenas, J.P., Quatrini, R., Esparza, M., Osorio, H., Duarte, F., Lefimil, C., Sepulveda, R., Jedlicki, E.and Holmes, D.S. (2010) Comparative Genomics Begins to Unravel the Ecophysiology of Bioleaching. Hydrometallurgy, 104, 471-476. [Google Scholar] [CrossRef] 
[23] Zhang, X., Liu, X., He, Q., Dong, W., Zhang, X., Fan, F., Peng, D., Huang, W. and Yin, H. (2016) Gene Turnover Contributes to the Evolutionary Adaptation of Acidi-thiobacillus caldus: Insights from Comparative Genomics. Frontiers in Microbiology, 7, Article ID: 1960. [Google Scholar] [CrossRef] [PubMed]
[24] Zhang, X., Feng, X., Tao, J.M., Ma, L.Y., Xiao, Y.H., Liang, Y.L., Liu, X.D. and Yin, H.Q. (2016) Comparative Genomics of the Extreme Acidophile Acidithiobacillus thiooxidans Reveals Intraspecific Divergence and Niche Adaptation. International Journal of Molecular Sciences, 17, Article ID: 1355. [Google Scholar] [CrossRef] [PubMed]
[25] Osorio, H., Martinez, V., Nieto, P.A., Holmes, D.S. and Quatrini, R. (2008) Microbial Iron Management Mechanisms in Extremely Acidic Environments: Comparative Genomics Evidence for Diversity and Versatility. BMC Microbiology, 8, 18. [Google Scholar] [CrossRef] [PubMed]
[26] Quatrini, R., Jedlicki, E. and Holmes, D.S. (2005) Genomic Insights into the Iron Uptake Mechanisms of the Biomining Microorgan-ism Acidithiobacillus ferrooxidans. Journal of Industrial Microbiology and Biotechnology, 32, 606-614. [Google Scholar] [CrossRef] [PubMed]
[27] Pablo Cardenas, J., Moya, F., Covarrubias, P., Shmaryahu, A., Levican, G., Holmes, D.S. and Quatrini, R. (2012) Comparative Genomics of the Oxidative Stress Response in Bi-oleaching Microorganisms. Hydrometallurgy, 127, 162-167. [Google Scholar] [CrossRef] 
[28] Feng, S., Hou, S., Cui, Y., Tong, Y. and Yang, H. (2020) Metabolic Transcriptional Analysis on Copper Tolerance in Moderate Thermophilic Bioleaching Microorganism Acidi-thiobacillus caldus. Journal of Industrial Microbiology and Biotechnology, 47, 21-33. [Google Scholar] [CrossRef] [PubMed]
[29] Gupta, P. and Diwan, B. (2017) Bacterial Exopolysaccharide Mediated Heavy Metal Removal: A Review on Biosynthesis, Mechanism and Remediation Strategies. Biotechnology Re-ports, 13, 58-71. [Google Scholar] [CrossRef] [PubMed]
[30] Yin, Z., Feng, S., Tong, Y. and Yang, H. (2019) Adaptive Mecha-nism of Acidithiobacillus thiooxidans CCTCC M 2012104 under Stress during Bioleaching of Low-Grade Chalcopyrite Based on Physiological and Comparative Transcriptomic Analysis. Journal of Industrial Microbiology and Biotechnolo-gy, 46, 1643-1656. [Google Scholar] [CrossRef] [PubMed]
[31] Li, B., Lin, J., Mi, S. and Lin, J. (2010) Arsenic Resistance Op-eron Structure in Leptospirillum ferriphilum and Proteomic Response to Arsenic Stress. Bioresource Technology, 101, 9811-9814. [Google Scholar] [CrossRef] [PubMed]
[32] Panyushkina, A., Matyushkina, D. and Pobeguts, O. (2020) Understanding Stress Response to High-Arsenic Gold-Bearing Sulfide Concentrate in Extremely Metal-Resistant Aci-dophile Sulfobacillus thermotolerans. Microorganisms, 8, Article ID: 1076. [Google Scholar] [CrossRef] [PubMed]
[33] Sadeghieh, S.M., Ahmadi, A. and Hosseini, M.R. (2020) Effect of Water Salinity on the Bioleaching of Copper, Nickel and Cobalt from the Sulphidic Tailing of Golgohar Iron Mine, Iran. Hydrometallurgy, 198, Article ID: 105503. [Google Scholar] [CrossRef] 
[34] Zammit, C.M., Mangold, S., Jonna, V.R., Mutch, L.A., Wat-ling, H.R., Dopson, M. and Watkin, E.L.J. (2012) Bioleaching in Brackish Waters-Effect of Chloride Ions on the Acido-phile Population and Proteomes of Model Species. Applied Microbiology and Biotechnology, 93, 319-329. [Google Scholar] [CrossRef] [PubMed]
[35] Ye, M., Yan, P., Sun, S., Han, D., Xiao, X., Zheng, L., Huang, S., Chen, Y. and Zhuang, S. (2017) Bioleaching Combined Brine Leaching of Heavy Metals from Lead-Zinc Mine Tailings: Transformations during the Leaching Process. Chemosphere, 168, 1115-1125. [Google Scholar] [CrossRef] [PubMed]
[36] 郝闯, 唐兵, 唐晓峰. 嗜盐微生物的工业应用研究及进展[J]. 生物资源, 2019, 41(4): 281-288.
[37] 毛振华, 孙见行, 周文博, 王玉光, 周洪波, 程海娜. 生物冶金中耐盐浸矿微生物的研究进展[J]. 微生物学通报, 2020, 47(9): 321-328.
[38] Rea, S.M., McSweeney, N.J., Degens, B.P., Morris, C., Siebert, H.M. and Kaksonen, A.H. (2015) Salt-Tolerant Microorganisms Potentially Useful for Bioleaching Operations Where Fresh Water Is Scarce. Minerals Engineering, 75, 126-132. [Google Scholar] [CrossRef] 
[39] Rivera-Araya, J., Huynh, N.D., Kaszuba, M., Chavez, R., Schlomann, M. and Levican, G. (2020) Mechanisms of NaCl-Tolerance in Acidophilic Iron-Oxidizing Bacteria and Ar-chaea: Comparative Genomic Predictions and Insights. Hydrometallurgy, 194, Article ID: 105334. [Google Scholar] [CrossRef] 
[40] Khaleque, H.N., Shafique, R., Kaksonen, A.H., Boxall, N.J. and Watkin, E.L.J. (2018) Quantitative Proteomics Using SWATH-MS Identifies Mechanisms of Chloride Tolerance in the Halophilic Acidophile Acidihalobacter Prosperus DSM 14174. Research in Microbiology, 169, 638-648. [Google Scholar] [CrossRef] [PubMed]
[41] Xu, Y., Yin, H.Q., Jiang, H.D., Liang, Y.L., Guo, X., Ma, L.Y., Xiao, H.H. and Liu, X.D. (2013) Comparative Study of Nickel Resistance of Pure Culture and Co-Culture of Acidithio-bacillus thiooxidans and Leptospirillum ferriphilum. Archives of Microbiology, 195, 637-646. [Google Scholar] [CrossRef] [PubMed]
[42] Akinci, G. and Guven, D.E. (2011) Bioleaching of Heavy Metals Contaminated Sediment by Pure and Mixed Cultures of Acidithiobacillus spp. Desalination, 268, 221-226. [Google Scholar] [CrossRef] 
[43] Nurmi, P., Özkaya, B., Kaksonen, A.H., Tuovinen, O.H. and Pu-hakka, J.A. (2009) Inhibition Kinetics of Iron Oxidation by Leptospirillum ferriphilum in the Presence of Ferric, Nickel and Zinc Ions. Hydrometallurgy, 97, 137-145. [Google Scholar] [CrossRef] 
[44] Li, J., Tong, L., Xia, Y., Yang, H., Sand, W., Xie, H., Lan, B., Zhong, S. and Auwalu, A. (2020) Microbial Synergy and Stoichiometry in Heap Biooxidation of Low-Grade Porphyry Arsenic-Bearing Gold Ore. Extremophiles, 24, 355-364. [Google Scholar] [CrossRef] [PubMed]
[45] Zheng, X.C. and Li, D.W. (2016) Synergy between Rhizobium phaseoli and Acidithiobacillus ferrooxidans in the Bioleaching Process of Copper. Biomed Research International, 2016, Article ID: 9384767. [Google Scholar] [CrossRef] [PubMed]
[46] Ulloa, R., Moya-Beltran, A., Rojas-Villalobos, C., Nunez, H., Chiac-chiarini, P., Donati, E., Giaveno, A. and Quatrini, R. (2019) Domestication of Local Microbial Consortia for Efficient Recovery of Gold Through Top-Down Selection in Airlift Bioreactors. Frontiers in Microbiology, 10, 14. [Google Scholar] [CrossRef] [PubMed]
[47] Ma, L., Wang, H., Wu, J., Wang, Y., Zhang, D. and Liu, X. (2019) Metatranscriptomics Reveals Microbial Adaptation and Resistance to Extreme Environment Coupling with Bioleaching Performance. Bioresource Technology, 280, 9-17. [Google Scholar] [CrossRef] [PubMed]
[48] Jiang, H.D., Liang, Y.L., Yin, H.Q., Xiao, Y.H., Guo, X., Xu, Y., Hu, Q., Liu, H.W. and Liu, X.D. (2015) Effects of Arsenite Resistance on the Growth and Functional Gene Expres-sion of Leptospirillum ferriphilum and Acidithiobacillus thiooxidans in Pure Culture and Coculture. Biomed Research International, 2015, Article ID: 203197. [Google Scholar] [CrossRef] [PubMed]
[49] Li, X., Wang, H., Feng, X., Chen, J., Mao, Z., Li, G. and Yang, C. (2019) Progresses in Measurement Technologies of Heterogeneous Characteristics in Multiphase Reactors. Chemical Industry and Engineering Progress, 38, 45-71.
[50] Bitog, J.P., Lee, I.B., Lee, C.G., Kim, K.S., Hwang, H.S., Hong, S.W., Seo, I.H., Kwon, K.S. and Mostafa, E. (2011) Application of Computational Fluid Dynamics for Modeling and Designing Photobioreactors for Microalgae Production: A Review. Computers and Electronics in Agriculture, 76, 131-147. [Google Scholar] [CrossRef] 
[51] Duan, X., Feng, X., Peng, C., Yang, C. and Mao, Z. (2020) Numerical Simulation of Micro-Mixing in Gas-Liquid and Solid-Liquid Stirred Tanks with the Coupled CFD-E-Model. Chinese Journal of Chemical Engineering, 28, 2235-2247. [Google Scholar] [CrossRef] 
[52] Mousavi, S.M., Jafari, A., Chegini, S. andTurunen, I. (2009) CFD Simulation of Mass Transfer and Flow Behaviour around a Single Particle in Bioleaching Process. Process Biochemistry, 44, 696-703. [Google Scholar] [CrossRef] 
[53] Cheron, J., Loubiere, C., Delaunay, S., Guezennec, A.-G. and Olmos, E. (2020) CFD Numerical Simulation of Particle Suspension and Hydromechanical Stress in Various Designs of Multi-Stage Bioleaching Reactors. Hydrometallurgy, 197, Article ID: 105490. [Google Scholar] [CrossRef] 
[54] Zheng, C., Huang, Y., Guo, J., Cai, R., Zheng, H., Lin, C. and Chen, Q. (2018) Investigation of Cleaner Sulfide Mineral Oxidation Technology: Simulation and Evaluation of Stirred Bioreactors for Gold-Bioleaching Process. Journal of Cleaner Production, 192, 364-375. [Google Scholar] [CrossRef] 

为你推荐