ICG/PDA纳米粒的合成及其光热–光动力协同抑癌效应

Synthesis of ICG/PDA Nanoparticles and Their Photothermal-Photodynamic Synergistic Antitumor Effect

摘要: 在肿瘤治疗领域中，纳米载药体系的研究与开发对于提高肿瘤治疗的精准度和有效性具有非常重要的意义，但材料本身存在的生物相容性、肿瘤的异质性和复杂性以及可见光波段光源穿透深度不够等问题始终限制着肿瘤治疗效果的提高。本研究在聚多巴胺聚合过程中加入吲哚菁绿，通过π-π堆积和疏水相互作用实现ICG的装载，制备出可用于光热/光动力联合治疗的纳米载药体系。结果表明，所制备的ICG/PDA材料具有较好的光热转换能力和光热稳定性，在功率密度为1 W/cm²的光照下，溶液温度能够在1 min内迅速上升至50℃，此外，ICG/PDA较ICG有更快的活性氧产生速度和更多的活性氧产量。人卵巢癌细胞SKOV3细胞实验结果表明，所制备的ICG/PDA材料在未光照的条件下具有较高的生物相容性，而在808 nm近红外光照下具有明显的光照抑制作用。综上所述，所制备的肿瘤抑制性ICG/PDA纳米复合材料具有用于肿瘤光热/光动力联合治疗的潜力。

Abstract: In the field of tumor therapy, the research and development of nano-drug delivery systems are of great significance to improve the accuracy and effectiveness of tumor therapy, but the biocompati-bility of the material itself, the heterogeneity and complexity of tumors, and visible light problems such as insufficient penetration depth of the band light source have always limited the improvement of tumor treatment effects. In this study, indocyanine green was introduced in the process of polydopamine polymerization, and ICG was loaded through π-π stacking and hydrophobic interaction to prepare a nano-drug loading system that can be used for photothermal/photodynamic combined therapy. The results showed that the prepared ICG/PDA nanoparticles had good photother-mal conversion ability and photothermal stability with a temperature rise to 50˚C in 1 min under the irradiation of 1 W/cm² NIR, meanwhile, a faster active oxygen generation rate and more active oxygen production were exhibited than those by free ICG. Finally, the experimental results of human ovarian cancer cells (SKOV3) showed that the prepared ICG/PDA material possessed the excellent biocompatibility under condition without NIR irradiation, and exhibited the obvious tumor cell inhibition under 808 nm NIR irradiation. In summary, the prepared tumor inhibitory ICG/PDA nanoparticles have the potential to be used in photothermal/photodynamic combined therapy.