辐射诱变选育中华茉莉新品系

Breeding of New Strains of Chinese Jasmine by Radiation Mutation

摘要: 本研究用γ射线(⁶⁰Co)辐射诱变处理“禾苗-1”中华茉莉胚性愈伤，茎、叶2种材料胚性愈伤诱导，茎的胚性愈伤诱导率高，达到100%。愈伤培养42 h胚性细胞分化，75~99 h胚性细胞大量形成，为γ射线最佳辐射处理时间，40 Gy辐射剂量较接近半致死剂量。变异体生根试管苗218株，经田间优选获得1个优系，暂定名为“禾苗-2”中华茉莉，连续2 y生长发育检测，性状稳定，明显高于亲本和对照。

Abstract: In this study, γ-ray (⁶⁰Co) radiation mutagenesis was used to treat embryogenic callus of “He Miao-1” Chinese Jasmine. The embryogenic callus of stem and leaf were induced, and the induction rate of embryogenic callus of stem was high, reaching 100%. The callus was cultured for 42 hours of embryogenic cell differentiation, and a large number of embryogenic cells were formed at 75~99 hours. This is the best time for γ-ray radiation treatment, and the 40 Gy radiation dose is close to the half-lethal dose. 218 mutant rooting test-tube seedlings, one superior line was obtained by field optimization, tentatively named “He Miao-2” Chinese Jasmine, and the growth and development test for 2 years continuously showed stable traits, which were significantly higher than that of the parent and the control.