基于乙酰胆碱酯酶的一次性可抛纸基酶传感器应用于杀螟硫磷的检测研究
Study on the Application of Acetylcholinesterase-Based Disposable Paper-Based Sensor to the Detection of Fenitrothion
DOI: 10.12677/NAT.2021.113025, PDF,    国家自然科学基金支持
作者: 丁 睿*, 刘 帅*, 叶 露, 潘 婧, 鲍 宁, 刘 扬#：南通大学公共卫生学院，江苏 南通
关键词: 杀螟硫磷；纸基传感器；乙酰胆碱酯酶； Fenitrothion； Paper-Based Sensor； Acetylcholinesterase
摘要: 本研究旨在研发了一种应用于有机磷农药快速、准确、一次性可抛的传感装置。用碳胶/氧化铟锡玻璃(ITO)作为工作电极，碳和银作为对电极和参比电极构成基于三电极系统。纳米金铂–石墨烯材料修饰在工作电极表面用来固定乙酰胆碱酯酶(AChE)和加速电子传递速度。以氯化乙酰胆碱为底物，通过AChE酶催化反应检测底物杀螟硫磷。结果表明，使用差分脉冲伏安法检测杀螟硫磷，其浓度从1.0 × 10−7到1.5 × 10−5 mol/L范围内，氧化峰电流的降低程度与杀螟硫磷浓度的对数成正比，检测限低至5.4 × 10−8 mol/L。该酶传感器有希望成为快速检测有机磷农药的新方法。
Abstract: The purpose is to develop a rapid, accurate, disposable sensing device applied to organophosphrus pesticides (OPs). Using carbon glue/indium tin oxide glass (ITO) as working electrode, carbon glue and silver glue as counter and reference electrode, a three-electrode system was formed. Gold/ platinum-graphene nanocomposites were modified to immobilize acetylcholinesterase (AChE) and enhance electron transfer rate. Acetylcholine chloride was used as substrate to detect fenitrothion by AChE enzyme catalyzed reaction. The results showed that the reduction of oxidation peak current was directly proportional to the logarithm of the concentration of fenitrothion in the range of 1.0 × 10−7 to 1.5 × 10−5 mol/L by differential pulse voltammetry (DPV). The detection limit was as low as 5.4 × 10−8 mol/L. The enzyme sensor is expected to be a new method for rapid and economical detection of OPs.
文章引用：丁睿, 刘帅, 叶露, 潘婧, 鲍宁, 刘扬. 基于乙酰胆碱酯酶的一次性可抛纸基酶传感器应用于杀螟硫磷的检测研究[J]. 纳米技术, 2021, 11(3): 218-229. https://doi.org/10.12677/NAT.2021.113025

参考文献

[1] Amine, A., Arduini, F., Moscone, D. and Palleschi, G. (2016) Recent Advances in Biosensors Based on Enzyme Inhibi-tion. Biosensors and Bioelectronics, 76, 180-194. [Google Scholar] [CrossRef] [PubMed]
[2] Govindhan, M., Adhikari, B. and Chen, A. (2014) Nanomaterials-Based Electrochemical Detection of Chemical Contaminants. RSC Ad-vances, 4, 63741-63760. [Google Scholar] [CrossRef] 
[3] Yu, J., Zhang, Y. and Liu, S. (2014) Enzy-matic Reactivity of Glucose Oxidase Confined in Nanochannels. Biosensors and Bioelectronics, 55, 307-312. [Google Scholar] [CrossRef] [PubMed]
[4] Werkmeister, F.X., Koide, T. and Nickel, B.A. (2016) Ammonia Sensing for Enzymatic Urea Detection Using Organic Field Effect Transistors and a Semipermeable Membrane. Journal of Materials Chemistry B, 41, 162-168. [Google Scholar] [CrossRef] [PubMed]
[5] Romanholo, P., Razzino, C.A., Raymundo-Pereira, P.A., Prado, T.M., Machado, S. and Sgobbi, L.F. (2021) Biomimetic Electrochemical Sensors: New Horizons and Challenges in Bio-sensing Applications. Biosensors and Bioelectronics, 185, 113242-113268. [Google Scholar] [CrossRef] [PubMed]
[6] Das, P., Das, M., Chinnadayyala, S.R., Singha, I.M. and Goswami, P. (2016) Recent Advances on Developing 3rd Generation Enzyme Electrode for Biosensor Applications. Biosensors and Bioelectronics, 79, 386-397. [Google Scholar] [CrossRef] [PubMed]
[7] Sarma, A. K., Vatsyayan, P., Goswami, P. and Minteer, S.D. (2009) Recent Advances in Material Science for Developing Enzyme Electrodes. Biosensors and Bioelectronics, 24, 2313-2322. [Google Scholar] [CrossRef] [PubMed]
[8] Yu, C., Zhu, Z., Wang, L., Wang, Q., Bao, N. and Gu, H. (2014) A New Disposable Electrode for Electrochemical Study of Leukemia k562 Cells and Anticancer Drug Sensitivity Test. Bio-sensors and Bioelectronics, 53, 142-147. [Google Scholar] [CrossRef] [PubMed]
[9] Dago, À., Navarro, J., Ariño, C., Díaz-Cruz, J.M. and Esteban, M. (2015) Carbon Nanotubes and Graphene Modified Screen-Printed Carbon Electrodes as Sensitive Sensors for the Deter-mination of Phytochelatins in Plants Using Liquid Chromatography with Amperometric Detection. Journal of Chroma-tography A, 1409, 210-217. [Google Scholar] [CrossRef] [PubMed]
[10] Regiart, M., Fernández-Baldo, M.A., Spotorno, V.G., Bertolino, F.A. and Raba, J. (2013) Ultra Sensitive Microfluidic Immunosensor for Determination of Clenbuterol in Bovine Hair Samples Using Electrodeposited Gold Nanoparticles and Magnetic Micro Particles as Bio-Affinity Platform. Biosensors and Bioelectronics, 41, 211-217. [Google Scholar] [CrossRef] [PubMed]
[11] Aurbek, N., Thiermann, H., Szinicz, L., Eyer, P. and Worek, F. (2006) Analysis of Inhibition, Reactivation and Aging Kinetics of Highly Toxic Organophosphorus Compounds with Human and Pig Acetylcholinesterase. Toxicology, 224, 91-99. [Google Scholar] [CrossRef] [PubMed]
[12] Tsai, J.Z., Chen, C.J., Settu, K., Lin, Y.F. and Liu, J.T. (2015) Screen-Printed Carbon Electrode-Based Electrochemical Im-munosensor for Rapid Detection of Microalbuminuria. Biosensors and Bioelectronics, 77, 1175-1182. [Google Scholar] [CrossRef] [PubMed]
[13] Hayat, A. and Marty, J.L. (2014) Disposable Screen Printed Elec-trochemical Sensors: Tools for Environmental Monitoring. Sensors (Basel, Switzerland), 14, 10432-10453. [Google Scholar] [CrossRef] [PubMed]
[14] Dreyer, D.R., Park, S., Bielawski, C.W. and Ruoff, R.S. (2010) The Chemistry of Graphene Oxide. Chemical Society Reviews, 39, 228-240. [Google Scholar] [CrossRef] 
[15] Ferrari, A.C. and Basko, D.M. (2013) Raman Spectroscopy as a Versatile Tool for Studying the Properties of Graphene. Nature Nanotechnology, 8, 235-246. [Google Scholar] [CrossRef] [PubMed]
[16] Geim, A.K. (2009) Graphene: Status and Prospects. Science (New York, N.Y.), 324, 1530-1534. [Google Scholar] [CrossRef] [PubMed]
[17] Geim, A.K. and Novoselov, K.S. (2007) The Rise of Graphene. Na-ture Materials, 6, 183-191. [Google Scholar] [CrossRef] [PubMed]
[18] Novoselov, K.S., Geim, A.K., Morozov, S.V., Jiang, D., Katsnelson, M.I., Grigorieva, I.V., Dubonos, S.V. and Firsov, A.A. (2005) Two-Dimensional Gas of Massless Dirac Fermions in Gra-phene. Nature, 438, 197-200. [Google Scholar] [CrossRef] [PubMed]
[19] Novoselov, K.S., Geim, A.K., Morozov, S.V., Jiang, D., Zhang, Y., Du-bonos, S.V., Grigorieva, I.V. and Firsov, A.A. (2004) Electric Field Effect in Atomically Thin Carbon Films. Science (New York, N.Y.), 306, 666-669. [Google Scholar] [CrossRef] [PubMed]
[20] Brownson, D.A. and Banks, C.E. (2010) Graphene Electrochemistry: An Overview of Potential Applications. The Analyst, 135, 2768-2778. [Google Scholar] [CrossRef] [PubMed]
[21] Fakhari, A.R., et al. (2014) Development of an Electrochemical Sensor Based on Reduced Graphene Oxide Modified Screen-Printed Carbon Electrode for the Determination of Buprenorphine. Electroanalysis, 26, 2474-2483. [Google Scholar] [CrossRef] 
[22] Shan, C., Yang, H., Song, J., Han, D., Ivaska, A. and Niu, L. (2009) Direct Electrochemistry of Glucose Oxidase and Biosensing for Glucose Based on Grapheme. Analytical Chemistry, 81, 2378-2382. [Google Scholar] [CrossRef] [PubMed]
[23] Zhou, M., Zhai, Y. and Dong, S. (2009) Electrochemical Sensing and Biosensing Platform Based on Chemically Reduced Graphene Oxide. Analytical Chemistry, 81, 5603-5613. [Google Scholar] [CrossRef] [PubMed]
[24] Dao, V.D., Choi, Y., Yong, K., Larina, L.L. and Choi, H.S. (2015) Gra-phene-Based Nanohybrid Materials as the Counter Electrode for Highly Efficient Quantum-Dot-Sensitized Solar Cells. Carbon, 84, 383-389. [Google Scholar] [CrossRef] 
[25] Feng, L., Guo, G., Peng, H., Wang, X., Zhang, C. and Zhang, J. (2011) Preparation of PtAg Alloy Nanoisland/Graphene Hybrid Composites and Its High Stability and Catalytic Activity in Methanol Electro-Oxidation. Nanoscale Research Letters, 6, Article No. 551. [Google Scholar] [CrossRef] 
[26] Lu, W.B., Ge, J., Lin, T., Cao, X.W., Dong, J. and Qian, W.P. (2014) Large-Scale Synthesis of Ultrathin Au-Pt Nanowires Assembled on Thionine/Graphene with High Conductivity and Sensitivity for Electrochemical Immunosensor. Electrochimica Acta, 130, 335-343. [Google Scholar] [CrossRef] 
[27] Alkasir, R., Ganesana, M., Won, Y.H., Stanciu, L. and An-dreescu, S. (2011) Enzyme Functionalized Nanoparticles for Electrochemical Biosensors: A Comparative Study with Ap-plications for the Detection of Bisphenol a. Biosensors and Bioelectronics, 26, 43-49. [Google Scholar] [CrossRef] [PubMed]
[28] He, W., Liu, Y., Yuan, J., Yin, J.J., Wu, X., Hu, X., Zhang, K., Liu, J., Chen, C., Ji, Y. and Guo, Y. (2011) Au@Pt Nanostructures as Oxidase and Peroxidase Mimetics for Use in Immu-noassays. Biomaterials, 32, 1139-1147. [Google Scholar] [CrossRef] [PubMed]
[29] He, Y., Zhang, S., Zhang, X., Baloda, M., Gurung, A.S., Xu, H., Zhang, X. and Liu, G. (2011) Ultrasensitive Nucleic Acid Biosensor Based on Enzyme-Gold Nanoparticle Dual Label and Lateral Flow Strip Biosensor. Biosensors and Bioelectronics, 26, 2018-2024. [Google Scholar] [CrossRef] [PubMed]
[30] Lv, M., Liu, Y., Geng, J.H., Kou, X.H., Xin, Z.H. and Yang, D.Y. (2018) Engineering Nanomaterials-Based Biosesors for Food Safety Detection. Biosensors and Bioelectronics, 106, 122-128. [Google Scholar] [CrossRef] [PubMed]
[31] Xie, C., Li, H., Li, S., Wu, J. and Zhang, Z. (2010) Sur-face Molecular Self-Assembly for Organophosphate Pesticide Imprinting in Electropolymerized Poly(p-aminothiophenol) Membranes on a Gold Nanoparticle Modified Glassy Carbon Electrode. Analytical Chemistry, 82, 241-249. [Google Scholar] [CrossRef] [PubMed]
[32] Urbek, N., Thiermann, H., Szinicz, L., Eyer, P. and Worek, F. (2006) Analysis of Inhibition, Reactivation and Aging Kinetics of Highly Toxic Organophosphorus Compounds with Human and Pig Acetylcholinesterase. Toxicology, 224, 91-99. [Google Scholar] [CrossRef] [PubMed]
[33] Thiermann, H., Szinicz, L., Eyer, P., Felgenhauer, N. and Worek, F. (2007) Lessons to Be Learnt from Organophosphorus Pesticide Poisoning for the Treatment of Nerve Agent Poisoning. Toxicology, 233, 145-154. [Google Scholar] [CrossRef] [PubMed]
[34] Abdollahi, M. and Karami-Mohajeri, S. (2012) A Comprehensive Review on Experimental and Clinical Findings in Intermediate Syndrome Caused by Organophosphate Poisoning. Toxi-cology and Applied Pharmacology, 258, 309-314. [Google Scholar] [CrossRef] [PubMed]
[35] Banks, C.N. and Lein, P.J. (2012) A Review of Experimental Evi-dence Linking Neurotoxic Organophosphorus Compounds and Inflammation. Neurotoxicology, 33, 575-584. [Google Scholar] [CrossRef] [PubMed]
[36] Pope, C.N. and Jing, L. (1997) Age-Related Differences in Sensi-tivity to Organophosphorus Pesticides. Environmental Toxicology and Pharmacology, 4, 309-314. [Google Scholar] [CrossRef] 
[37] Chowdhary, S., Bhattacharyya, R. and Banerjee, D. (2014) Acute Organophosphorus Poisoning. ClinicaChimicaActa, 431, 66-76. [Google Scholar] [CrossRef] [PubMed]
[38] Melgar, L.Z. and Machado, S.A.S. (2005) Determination of Fenitrothion in Commercial Formulations by Square Wave Voltammetry and UV-Vis Spectroscopy. Journal of the Bra-zilian Chemical Society, 16, 743-748. [Google Scholar] [CrossRef] 
[39] Subrahmanyam, P., Krishnapriya, B., Suvardhan, K., Rekha, D., Suneeta, Y., Jayaraj, B. and Chiranjeevi, P. (2007) Simple, Selective and Sensitive Spectrophotometric De-termination of Fenitrothion Using Novel Chromogenic Reagent. Journal of Hazardous Materials, 146, 51-57. [Google Scholar] [CrossRef] [PubMed]
[40] Kalyabina, V.P., Esimbekova, E.N., Kopylova, K.V. and Kra-tasyuk, V.A. (2021) Toxicology Reports Pesticides: Formulants, Distribution Pathways and Effects on Human Health—A Review. Toxicology Reports, 8, 1179-1192. [Google Scholar] [CrossRef] [PubMed]
[41] Yao, S., Meyer, A. and Henze, G. (1991) Comparison of Am-perometric and UV-Spectrophotometric Monitoring in the HPLC Analysis of Pesticides. Analytical Chemistry, 339, 207-211. [Google Scholar] [CrossRef] 
[42] Chauhan, N. and Pundir, C.S. (2014) Amperometric Determi-nation of Acetylcholine-A Neurotransmitter, by Chitosan/Gold-Coated Ferric Oxide Nanoparticles Modified Gold Elec-trode. Biosensors and Bioelectronics, 61, 1-8. [Google Scholar] [CrossRef] 
[43] Lu, L. and Xia, Y. (2015) Enzymatic Reaction Modulated Gold Nanorod End-to-End Self-Assembly for Ultrahigh Sensitively Colorimetric Sensing of Cholinesterase and Organophosphate Pes-ticides in Human Blood. Analytical Chemistry, 87, 8584-8591. [Google Scholar] [CrossRef] [PubMed]
[44] Yi, Y., Zhu, G., Liu, C., Huang, Y., Zhang, Y., Li, H., Zhao, J. and Yao, S. (2013) A Label-Free Silicon Quantum Dots-Based Photoluminescence Sensor for Ultrasensitive Detection of Pesticides. Analytical Chemistry, 85, 11464-11470. [Google Scholar] [CrossRef] [PubMed]
[45] Baby, T.T., Aravind, S.S.J., Arockiadoss, T., Rakhi, R.B. and Ramaprabhu, S. (2010) Metal Decorated Graphene Nanosheets as Immobiliza-tion Matrix for Amperometric Glucose Biosensor. Sensors and Actuators B: Chemical, 145, 71-77. [Google Scholar] [CrossRef] 
[46] Gan, N., Yang, X., Xie, D., Wu, Y. and Wen, W. (2010) A Dis-posable Organophosphorus Pesticides Enzyme Biosensor Based on Magnetic Composite Nano-Particles Modified Screen Printed Carbon Electrode. Sensors, 10, 625-638. [Google Scholar] [CrossRef] [PubMed]
[47] Nesakumar, N., Sethuraman, S., Krishnan, U.M. and Rayappan, J.B. (2016) Electrochemical Acetylcholinesterase Biosensor Based on ZnO Nanocuboids Modified Platinum Electrode for the Detection of Carbosulfan in Rice. Biosensors and Bioelectronics, 77, 1070-1077. [Google Scholar] [CrossRef] [PubMed]
[48] Sun, L.J., Feng, Q.M., Yan, Y.F., Pan, Z.Q., Li, X.H., Song, F.M., Yang, H., Xu, J.J., Bao, N. and Gu, H.Y. (2014) Paper-Based Electroanalytical Devices for in Situ Determination of Sal-icylic Acid in Living Tomato Leaves. Biosensors and Bioelectronics, 60, 154-160. [Google Scholar] [CrossRef] [PubMed]
[49] Bard, A.J. and Faulkner, L.R. (1980) Electrochemical Methods: Fundamentals and Applications. John Wiley and Sons, New York, 138-139.

为你推荐