RBMX在食管鳞状细胞癌中的相关性研究——RBMX对食管鳞状细胞癌细胞增殖和凋亡的影响

Study on the Correlation of RBMX in Esophageal Squamous Cell Carcinoma—Effects of RBMX on the Proliferation and Apoptosis of Esophageal Squamous Cell Carcinoma

摘要: RBMX (也称为hnRNP G)是hnRNP家族的成员，最早被鉴定为表观分子量为43 kDa的核蛋白。本研究目的主要是检验RBMX在ESCC中是否是肿瘤抑制因子的假设。对食管鳞状细胞癌组织进行免疫组织化学和蛋白质印迹分析。采用Kaplan-Meier法来分析ESCC患者的生存率。通过构建RBMX的过表达质粒，研究了RBMX在TE1食管鳞状细胞癌细胞系增殖中的作用。通过CCK-8和流式细胞术测定RBMX对增殖和凋亡的调节。免疫组化分析显示RBMX表达与Ki67呈负相关。生存分析显示RBMX表达与总体生存率显著相关(P < 0.001)。RBMX在TE1细胞中的过表达可导致CyclinD1 (细胞周期蛋白D1)、CDK4和p27水平降低和细胞增殖能力的下降。此外，RBMX的过表达可以诱导TE1细胞凋亡。这些发现表明RBMX参与了食管鳞状细胞癌的增殖，并且RBMX表达水平的升高可能表示食管鳞状细胞癌患者的良好预后。

Abstract: RBMX (also known as hnRNP G), is a member of hnRNP family and was first identified as a nuclear protein with an apparent molecular weight of 43 kDa. The current study was designed to test the hypothesis that RBMX is a tumor suppressor. Immunohistochemical and western blot analyses were performed in esophageal squamous cell carcinoma tissues. Survival analyses were performed by using the Kaplan-Meier method. The role of RBMX in proliferation was studied in esophageal squamous cell carcinoma cell lines of TE1 by constructing the RBMX overexpression plasmid. The regulation of RBMX on proliferation and apoptosis was determined by Cell Counting Kit-8 assays and Flow Cytometry. Immunohistochemical analysis showed that RBMX expression was negatively associated with Ki67. Survival analysis revealed that RBMX expression was significantly associated with overall survival (P < 0.001). Over-expression of RBMX in TE1 cells resulted in decreased CyclinD1, CDK4 and p27 levels and the capability for proliferation. In addition, over-expression of RBMX induces the Apoptosis of TE1 cells. These findings suggest that RBMX is involved in the proliferation of esophageal squamous cell carcinoma, and that elevated levels of RBMX expression may indicate a good prognosis for patients with esophageal squamous cell carcinoma.