维生素D摄取不足对大鼠睾丸生殖功能的影响的研究
Research on the Effect of Insufficient Vitamin D Intake on the Reproductive Function of Rat Testis
DOI: 10.12677/OJNS.2021.95072, PDF,   
作者: 李 婧*, 田 玫, 李 健#：湖南师范大学医学院，模式动物与干细胞生物学湖南省重点实验室，湖南 长沙；胡 鑫*：湖南师范大学医学院小分子靶向药物发现与创制湖南省重点实验室，湖南 长沙
关键词: 维生素D；男性生殖；精子发生；增殖；Vitamin D； Male Reproduction； Spermatogenesis； Proliferation
摘要: 研究维生素D摄取不足对大鼠睾丸生殖功能的影响。方法：建立低维生素D (VDI)和正常饮食(VD)动物模型，观察大鼠身长、体重和左右睾丸重量，检测大鼠血清指标，附睾精子密度及睾丸组织切片。结果：与VD组比较，VDI组体重，身长和左右侧睾丸重量的差异均无统计学意义(P > 0.05)。血清25(OH)D水平明显降低(P < 0.01)，血清碱性磷酸酶含量明显下降(P < 0.05)。附睾精子密度下降但无统计学差异(P > 0.05)。睾丸组织H & E染色和PCNA蛋白免疫组织化学染色的差异无统计学意义(P > 0.05)。结论：大鼠低维生素D饮食导致血清中25(OH)D水平明显降低，但是对大鼠睾丸生殖功能没有显著影响。
Abstract: Objective: To study the effect of insufficient vitamin D intake on rat testicular reproductive function. Methods: Establish low-vitamin D (VDI) and normal diet (VD) animal models; observe the rat’s body length, weight, and left and right testicular weights, and detect rat serum indicators, epididymal sperm density and testicular tissue sections. Results: Compared with the VD group, there were no statistically significant differences in body weight, length, and left and right testicular weights in the VDI group (P > 0.05). The serum 25(OH)D level was significantly reduced (P < 0.01), and the serum alkaline phosphatase content was significantly reduced (P < 0.05). The sperm density of the epididymis decreased but there was no statistical difference (P > 0.05). There was no statistically significant difference in H & E staining and PCNA protein immunohistochemical staining of testis tissue (P > 0.05). Conclusion: Low vitamin D diet in rats caused a significant decrease in serum 25(OH)D level, but it had no significant effect on rat testicular reproductive function.
文章引用：李婧, 胡鑫, 田玫, 李健. 维生素D摄取不足对大鼠睾丸生殖功能的影响的研究[J]. 自然科学, 2021, 9(5): 662-671. https://doi.org/10.12677/OJNS.2021.95072

参考文献

[1] Pilz, S., Frisch, S., Koertke, H., et al. (2010) Effect of Vitamin D Supplementation on Testosterone Levels in Men. Hormone and Metabolic Research, 43, 223-225. [Google Scholar] [CrossRef] [PubMed]
[2] Jensen, M.B. (2012) Vitamin D Metabolism, Sex Hormones, and Male Reproductive Function. Reproduction, 144, 135-152. [Google Scholar] [CrossRef] 
[3] Hammoud, A.O., Meikle, A.W., Peterson, C.M., Stanford, J., Gibson, M. and Carrell, D.T. (2012) 25-Hydroxy-Vitamin D Levels with Semen and Hormonal Parameters. Asian Journal of Andrology, 14, 855-859. [Google Scholar] [CrossRef] [PubMed]
[4] 邓小林, 黎衍敏, 杨小燕, 黄建荣, 郭树林, 宋乐明. 维生素D治疗特发性少弱精子症的有效性与安全性研究[J]. 中华男科学杂志, 2014, 20(12): 1082-1085.
[5] Idelevich, A., Michael, K., et al. (2011) 1,25(OH)2D3 Alters Growth Plate Maturation and Bone Architecture in Young Rats with Normal Renal Function. PLoS ONE, 6, e20772. [Google Scholar] [CrossRef] [PubMed]
[6] Muindi, J.R., Modzelewski, R.A., Peng, Y., Trump, D.L. and Johnson, C.S. (2004) Pharmacokinetics of 1Alpha,25-Dihydroxyvitamin D3 in Normal Mice after Systemic Exposure to Effective and Safe Antitumor Doses. Oncology, 66, 62-66. [Google Scholar] [CrossRef] [PubMed]
[7] Ramlau-Hansen, C.H., Moeller, U.K., Bonde, J.P., Olsen, J. and Thulstrup, A.M. (2011) Are serum Levels of Vitamin D Associated with Semen Quality? Results from a Cross-Sectional Study in Young Healthy Men. Fertility and Sterility, 95, 1000-1004. [Google Scholar] [CrossRef] [PubMed]
[8] Sood, S., Marya, R.K., Reghunandanan, R., et al. (1992) Effect of Vitamin D Deficiency on Testicular Function in the rat. Annals of Nutrition & Metabolism, 36, 203-208. [Google Scholar] [CrossRef] [PubMed]
[9] Sun, W., Chen, L., Wei, Z., Rong, W., Goltzman, D. and Miao, D.S. (2015) Active Vitamin D Deficiency Mediated by Extracellular Calcium and Phosphorus Results in male infertility in young mice. American Journal of Physiology: Endocrinology and Metabolism, 308, E51-E62. [Google Scholar] [CrossRef] [PubMed]
[10] Holick, M.F. (2008) Vitamin D and Sunlight: Strategies for Cancer Prevention and Other Health Benefits. Clinical Journal of the American Society of Nephrology, 3, 1548-1554. [Google Scholar] [CrossRef] 
[11] Hu, Z., Tao, S.S., Liu, H., Pan, G. and Zhang, Z.J.J. (2019) The As-sociation between Polymorphisms of Vitamin D Metabolic-Related Genes and Vitamin D3 Supplementation in Type 2 Diabetic Patients. 1-8.
[12] Norman, A.W., Mizwicki, M.T. and Norman, D.P.G. (2004) Steroid-Hormone Rapid Actions, Membrane Receptors and a Conformational Ensemble Model. Nature Reviews: Drug Discovery, 3, 27-41. [Google Scholar] [CrossRef] [PubMed]
[13] Jensen, M.B., Lawaetz, J.G., Andersson, A.-M., et al. (2016) Vitamin D Deficiency and Low Ionized Calcium Are Linked with Semen Quality and Sex Steroid Levels in Infertile Men. Human Reproduction, 31, 1875-1885. [Google Scholar] [CrossRef] [PubMed]
[14] Anic, G.M., Albanes, D., Rohrmann, S., et al. (2016) Association between Serum 25-Hydroxyvitamin D and Serum Sex Steroid Hormones among Men in NHANES. Clinical Endocrinology, 85, 258-266. [Google Scholar] [CrossRef] [PubMed]
[15] Wulaningsih, W., Van Hemelrijck, M., Michaelsson, K., et al. (2014) Association of Serum Inorganic Phosphate with Sex Steroid Hormones and Vitamin D in a Nationally Representative Sample of Men. Andrology, 2, 967-976. [Google Scholar] [CrossRef] [PubMed]
[16] Valimaki, V.-V., Alfthan, H., Ivaska, K.K., et al. (2004) Serum Estradiol, Testosterone, and Sex Hormone-Binding Globulin as Regulators of Peak Bone Mass and Bone Turnover Rate in Young Finnish Men. The Journal of Clinical Endocrinology and Metabolism, 89, 3785-3789. [Google Scholar] [CrossRef] [PubMed]
[17] Chin, K.-Y., Ima-Nirwana, S. and Ngah, W.Z.W. (2015) Vitamin D Is Significantly Associated with Total Testosterone and Sex Hormone-Binding Globulin in Malaysian Men. Aging Male, 18, 175-179. [Google Scholar] [CrossRef] [PubMed]
[18] Zanatta, L., Bouraima-Lelong, H., Delalande, C., Silva, F.R.M.B. and Carreau, S. (2011) Development. Regulation of aromatase expression by 1α,25(OH)2 Vitamin D3 in Rat Testicular Cells. Reproduction Fertility & Development, 23, 725-735. [Google Scholar] [CrossRef] 
[19] Tak, Y.J., Lee, J.G., Kim, Y.J., et al. (2015) Serum 25-Hydroxyvitamin D Levels and Testosterone Deficiency in Middle-Aged Korean Men: A Cross-Sectional Study. Asian Journal of Andrology, 17, 324-328. [Google Scholar] [CrossRef] 
[20] Mortimer, D., Barratt, C.L.R., Bjoerndahl, L., de Jager, C., Jequier, A.M. and Muller, C.H. (2013) What Should It Take to Describe a Substance or Product as Sperm-Safe. Human Reproduction Update, 19, I1-I45. [Google Scholar] [CrossRef] [PubMed]
[21] Nimptsch, K., Platz, E.A., Willett, W.C. and Giovannucci, E. (2012) Association between Plasma 25-OH Vitamin D and Testosterone Levels in Men. Clinical Endocrinology, 77, 106-112. [Google Scholar] [CrossRef] [PubMed]
[22] 岳凤鸣, 杜心欣, 高福禄, 庞晓静. 糖尿病对小鼠睾丸组织中PCNA表达的影响[J]. 中国男科学杂志, 2001, 15(3): 154-156.