枸杞子–丹参对rd10小鼠视网膜内质网应激PERK-ATF4-CHOP通路的影响

Effect of Lycium barbarum and Salvia miltiorrhiza on PERK-ATF4-CHOP Pathway in Endoplasmic Reticulum

摘要: 目的：观察枸杞子–丹参对rd10小鼠视网膜内质网应激PERK-ATF4-CHOP通路的影响。方法：将40只rd10小鼠随机平均分为5组：空白组、对照组、枸杞组、丹参组、杞参组，分别予以生理盐水、维生素A溶剂、枸杞子中药配方颗粒溶液、丹参中药配方颗粒溶液、枸杞子加丹参中药配方颗粒溶液灌胃；8只C57小鼠为野生组，灌胃生理盐水。给药28天后，Western blot法检测各组小鼠视网膜GRP78、ATF4、eIF2α、CHOP相对表达情况。结果：杞参组小鼠视网膜GRP78、ATF4、eIF2α、CHOP相对表达量均低于空白组、对照组、枸杞组、丹参组(P < 0.05)。结论：枸杞子–丹参可以通过调控内质网应激PERK-ATF4-CHOP通路，抑制rd10小鼠视网膜细胞的凋亡，维持视网膜正常功能结构，从而保护视功能。

Abstract: Objective: To observe the effect of Lycium barbarum-Salvia miltiorrhiza on the PERK-ATF4-CHOP pathway of rd10 mouse retinal endoplasmic reticulum stress. Methods: Forty rd10 mice were randomly divided into blank group, control group, Lycium barbarum group, Salvia miltiorrhiza group, and Lycium barbarum-Salvia miltiorrhiza group (the qishen group), and they were given intragastrically: normal saline, vitamin A solvent, wolfberry Chinese medicine formula granule solution, Salvia miltiorrhiza Chinese medicine formula granule solution, Lycium barbarum and Salvia miltiorrhiza granule solution by gavage; eight C57 mice were in the wild group and normal saline was gavage. After 28 days of administration, the relative expressions of GRP78, ATF4, eIF2α and CHOP in the retina of each group were detected by Western blot. Results: The relative expression levels of GRP78, ATF4, eIF2α, and CHOP in the retina of mice in the Qishen group were lower than those of the blank group, control group, lycium barbarum group, and Salvia miltiorrhiza group (P < 0.05). Conclusion: Lycium barbarum-Salvia miltiorrhiza can regulate the endoplasmic reticulum stress PERK-ATF4-CHOP pathway, inhibit the apoptosis of rd10 mouse retinal cells, maintain the normal functional structure of the retina, and protect visual function.