八种脱钙液对骨组织制片后HE染色质量影响的实践探究

Practical Exploration of the Effect of Eight Kinds of Decalcification Solutions on the Quality of HE Staining after Bone Tissue Slices

摘要: 目的：该文旨在比较运用八种骨组织脱钙液后，病理制片的HE染色效果，以找到一种更加高效，综合效果更好的方法应用于临床工作当中。方法：选取家兔股骨骨干部分24份，修成1.5 cm × 0.5 cm × 0.2 cm的骨块，平均分成A、B、C、D、E、F、G、H八组，分别用A、B、C、D、E、F、G、H八种脱钙液在常温下进行脱钙处理，记录脱钙过程的时间，最后比较八组样本的染色质量。结果：G组(JYBL-II脱钙液)脱钙时间24 h，时间最短，F组(JYBL-III脱钙液)染色效果最好。D组(福尔马林–甲酸溶液)、E组(Krajian改良Evan-Krajian脱钙液)及G组(JYBL-II脱钙液)染色效果欠佳。A组(10%硝酸溶液)、B组(5%甲酸溶液)、C组(Richmon Gelfonud-Hill脱钙液)和H组(JYBL-I脱钙液)染色效果一般。结论：在八种脱钙液中，骨组织制片后HE染色质量及效果最好的为JYBL-III脱钙液。

Abstract: Objective: This article aims to compare the HE staining effects of pathological slices after using eight kinds of bone tissue decalcification solutions, in order to find a more efficient and comprehensive method for clinical work. Methods: Twenty-four parts of femur diaphysis of rabbits were selected to form 1.5 cm × 0.5 cm × 0.2 cm bone blocks, which were evenly divided into eight groups A, B, C, D, E, F, G and H and treated with eight kinds of decalcification solutions A, B, C, D, E, F, G and H respectively at room temperature. The process of decalcification was recorded, and the staining quality of the samples in the eight groups was compared. Results: The decalcification time of group G (JYBL-II decalcification solution) was 24 hours, the shortest time, and the staining effect of group F (JYBL-III decalcification solution) was the best. The staining effect of group D (formalin-formic acid solution), group E (Krajian modified Evan-Krajian decalcification solution) and group G (JYBL-II decalcification solution) was not good. The staining effects of group A (10% nitric acid solution), group B (5% formic acid solution), group C (Richmon Gelfonud-Hill decalcification solution) and group H (JYBL-I decalcification solution) were average. Conclusion: Among the eight kinds of decalcification solutions, the best quality and effect of HE staining after bone tissue preparation is JYBL-III decalcification solution.