吉贝咳喘胶囊在治疗AECOPD中抗菌抗病毒有效成分的网络药理学研究

Network Pharmacology Study of Antibacterial and Antiviral Active Components of JibeiKechuan Capsule in Treatment of A-ECOPD

摘要: 目的：基于网络药理学方法分析吉贝咳喘胶囊有效成分抗菌抗病毒作用在治疗急性加重慢性阻塞性肺疾病(AECOPD)中的作用机制研究。方法：通过CNKI、TCMSP、SwissTargetPrediction、化源网等数据库检索搜集并筛选吉贝咳喘胶囊中吉祥草、黄芩、浙贝母、蛤壳、毛大丁草、麻黄、桑白皮、葶苈子、天竺黄、僵蚕、地龙11味中药的主要成分及靶点信息，用Cytoscape3.8.0软件构建药物–成分–靶点网络；通过GeneCards数据库搜集抗菌、抗病毒的相关靶点信息；借助Venny在线平台分析共有交集靶点并绘制韦恩图；通过STRING平台进行蛋白质相互作用分析，构建PPI网络；通过David数据库进行GO分析及KEGG信号通路富集分析(P < 0.05)。结果：共筛选获得吉贝咳喘胶囊161个主要成分、883个靶点，抗菌、抗病毒相关靶点6465个，共同交集靶点537个，筛选核心潜在靶点有ATK1、MAPK3、RELA、NF-κB1、PAK3CA、MAPK1、MAPK14、MAPK8、TNF等34个；获得GO生物过程358条、KEGG信号富集通路103条，主要涉及PI3K/Akt信号通路、MAPK信号通路、toll样受体信号通路、T细胞受体信号通路、TNF信号通路等。结论：本研究结果表明，吉贝咳喘胶囊可能通过各药材的活性成分作用于ATK1、MAPK3、RELA、NF-κB1、PAK3CA、TNF等关键靶标参与PI3K/Akt信号通路、MAPK信号通路、toll样受体信号通路、T细胞受体信号通路、TNF信号通路联合发挥抗菌、抗病毒作用，充分体现了吉贝咳喘胶囊在治疗AECOPD中多成分、多靶点、多通路的抗菌、抗病毒作用机制，为进一步深入阐明吉贝咳喘胶囊作用机制提供理论参考。

Abstract: Objective: To analyze the mechanism of antibacterial and antiviral effects of effective components of JibeiKechuan Capsule in the treatment of acute exacerbation of chronic obstructive pulmonary disease (AECOPD) based on network pharmacology. Methods: The main components and target information of 11 traditional Chinese medicines in JibeiKechuan capsule were collected and selected by CNKI, TCMSP, SwissTargetPrediction and Huayuan.com databases. Cytoscape3.8.0 software was used to construct a drug-component-target network. GeneCards database was used to collect antibacterial and antiviral target information. The Venny online platform was used to analyze common inter-section targets and draw A Venn diagram. Protein interaction analysis was conducted through STRING platform to build PPI network. GO analysis and KEGG signal pathway enrichment analysis were performed by David database (P < 0.05). Results: A total of 161 main components, 883 targets, 6465 antibacterial and antiviral related targets and 537 common intersection targets of JibeiKech-uan capsule were screened. 34 potential targets were screened, including ATK1, MAPK3, RELA, NF-κB1, PAK3CA, MAPK1, MAPK14, MAPK8 and TNF. 358 GO biological processes and 103 KEGG signal enrichment pathways were obtained, mainly involving PI3K/Akt signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway, T cell receptor signaling pathway, TNF signaling pathway, etc. Conclusion: The results of this study indicate that JibeiKecchuan capsule may be involved in PI3K/Akt signaling pathway, MAPK signaling pathway, Toll-like receptor signaling pathway, T cell receptor signaling pathway and TNF signaling pathway by acting on ATK1, MAPK3, RELA, NF-κB1, PAK3CA, TNF and other key targets through the active ingredients of various medicinal materials. The effect of virus fully reflects the antibacterial and antiviral mechanism of JibeiKechuan capsule in the treatment of AECOPD with multiple components, multiple targets and multiple pathways, providing theoretical reference for further elucidating the mechanism of Action of JibeiKechuan capsule.