基于网络药理学探讨吉贝咳喘汤抗炎的作用机制

Discussion on the Anti-Inflammatory Mechanism of JibeiKechuan Decoction Based on Network Pharmacology

摘要: 目的：运用网络药理学的方法研究经验复方吉贝咳喘汤干预炎症的成分–靶点–通路作用机制，旨在为其基础研究及临床应用提供依据。方法：通过ETCM数据库、PharmMapper数据库及SwissADME平台检索筛选出吉贝咳喘汤的活性成分及作用靶点，采用GeneCards、OMIM、DisGeNet数据库筛出炎症相关靶点，取两者交集靶点，将交集靶点利用STRING11.0获得蛋白互作关系，并通过Cytoscape3.7.2构建PPI网络，分析吉贝咳喘汤抗炎的关键靶点；采用Metascape平台进行GO、KEGG基因富集分析，得到潜在作用通路，并构建靶点–通路网络。结果：吉贝咳喘汤中筛选得到145个活性成分，其中涉及炎症作用靶点316个，经分析最终筛选出34个关键靶点；KEGG富集分析关键靶点主要被富集在AGE-RAGE、NF-κB、cAMP等相关通路，涉及炎症反应、防御调节等3609个相关生物过程。结论：基于网络药理学探讨了吉贝咳喘汤抗炎成分–靶点–通路的作用特点，为进一步开展吉贝咳喘汤抗炎作用机制研究提供了新的思路和方法。

Abstract: Objective: To use the method of network pharmacology to study the component-target-pathway mechanism of the empirical compound JibeiKechuan Decoction to interfere with inflammation, in order to provide a basis for its basic research and clinical application. Methods: Search and screen out the active ingredients and targets of JibeiKechuan Decoction through ETCM database, PharmMapper database and SwissADME platform. Use GeneCards, OMIM, and DisGeNet databases to screen out inflammation-related targets, take the intersection of the two targets and combine them. The target uses STRING 11.0 to obtain protein interaction relationships, and constructs a PPI net-work through Cytoscape 3.7.2 to analyze the key targets of JibeiKechuan Decoction for anti-inflammation; uses Metascape platform to perform GO and KEGG gene enrichment analysis to obtain potential pathways and construct them Target-access network. Results: 145 active ingredients were screened in JibeiKechuan Decoction, including 316 targets involved in inflammation, and finally 34 key targets were screened out after analysis; the key targets of KEGG enrichment analysis were mainly enriched in AGE-RAGE, NF-κB, cAMP and other related pathways, involving 3609 related biological processes, such as inflammatory response and defense regulation. Conclusion: Based on network pharmacology, the characteristics of the anti-inflammatory component- target-pathway of JibeiKechuan Decoction were discussed, which provided new ideas and methods for further research on the anti-inflammatory mechanism of JibeiKechuan Decoction.