乐胃饮加味方通过抑制PI3K/AKT/mTOR通路促进自噬抑制NCI-N87细胞生长的机制研究
Study on the Mechanism of Modified Leweiyin Promoting Autophagy and Inhibiting the Growth of NCI-N87 Cells by Inhibiting PI3K/Akt/mTOR Pathway
DOI: 10.12677/TCM.2021.106109, PDF,    科研立项经费支持
作者: 叶 芸, 潘洁莉, 徐 珊, 朱飞叶*：浙江中医药大学，浙江 杭州；徐燕立：杭州传承中医门诊部，浙江 杭州
关键词: 乐胃饮加味方；NCI-N87细胞；自噬；PI3K/AKT/mTOR；Modified Le Weiyin； NCI-N87 Cells； Autophagy； PI3K/AKT/mTOR
摘要: 目的：探讨乐胃饮加味方对人胃癌NCI-N87细胞的作用机制。方法：采用不同浓度乐胃饮加味方处理NCI-N87细胞，透射电镜检测细胞超微结构改变，qRT-PCR检测自噬相关基因Beclin1、p62、凋亡相关基因Caspaese-3及PI3K、AKT和mTOR mRNA表达。结果：与对照组比较，乐胃饮加味方组细胞生长状态不良，胞内出现大量自噬小体，Beclin1 mRNA表达升高，p62 mRNA表达下降，Caspaese-3 mRNA表达增加，PI3K、AKT和mTOR mRNA表达均下降。结论：乐胃饮加味方可能通过抑制PI3K/AKT/mTOR信号通路促进自噬，诱导肿瘤细胞凋亡，从而抑制胃癌发展。
Abstract: Objective: To investigate the mechanism of modified Leweiyin on human gastric cancer NCI-N87 cells. Methods: NCI-N87 cells were treated with different concentrations of modified Leweyin. The ultrastructural changes were detected by transmission electron microscope, and the mRNA expressions of autophagy related genes Beclin1, p62, apoptosis related gene Caspase-3, PI3K, Akt and mTOR were detected by qRT-PCR. Results: Compared with the control group, the cells in the modified Lewiyin group had poor growth status, a large number of autophagy bodies appeared in the cells, the expression of Beclin1 mRNA increased, the expression of p62 mRNA decreased, the expression of caspase-3 mRNA increased, and the expression of PI3K, AKT and mTOR mRNA decreased. Conclusion: Modified Leweyin may promote autophagy and induce apoptosis by inhibiting PI3K/AKT/mTOR signal pathway, so as to inhibit the development of gastric cancer.
文章引用：叶芸, 徐燕立, 潘洁莉, 徐珊, 朱飞叶. 乐胃饮加味方通过抑制PI3K/AKT/mTOR通路促进自噬抑制NCI-N87细胞生长的机制研究[J]. 中医学, 2021, 10(6): 781-787. https://doi.org/10.12677/TCM.2021.106109

参考文献

[1] Chen, W., Zheng, R., Baade, P.D., et al. (2016) Cancer Statistics in China, 2015. CA: A Cancer Journal for Clinicians, 66, 115-132. [Google Scholar] [CrossRef] [PubMed]
[2] 苏羚子, 焦丽静, 许玲. 中医药治疗胃癌临床研究进展[J]. 世界临床药物, 2021, 42(5): 335-339.
[3] 朱君华, 徐珊. 乐胃饮治疗慢性萎缩性胃炎临床研究[J]. 中华中医药杂志, 2007, 22(8): 526-527.
[4] Onorati, A.V., Dyczynski, M., Ojha, R., et al. (2018) Targeting Autophagy in Cancer. Cancer, 124, 3307-3318. [Google Scholar] [CrossRef] [PubMed]
[5] 张升, 吴友苹, 顾利强, 等. 细胞自噬进程的分子信号通路研究进展[J]. 生命的化学, 2018, 38(2): 213-223.
[6] Ouyang, G., Xiong, L., Liu, Z., et al. (2018) Inhibition of Autophagy Potentiates the Apoptosis-Inducing Effects of Photodynamic Therapy on Human Colon Cancer Cells. Photodiagnosis and Photodynamic Therapy, 21, 396-403. [Google Scholar] [CrossRef] [PubMed]
[7] Kang, R., Zeh, H.J., Lotze, M.T., et al. (2011) The Beclin 1 Network Regulates Autophagy and Apoptosis. Cell Death & Differentiation, 18, 571-580. [Google Scholar] [CrossRef] [PubMed]
[8] Won, K.Y., Kim, G.Y., Lim, S.J., et al. (2015) Autophagy Is Related to the Hedgehog Signaling Pathway in Human Gastric Adenocareinoma: Prognostic Significance of Beclin1 and Gli2 Expression in Human Gastric Adeno Carcinoma. Pathology-Research and Practice, 211, 308-315. [Google Scholar] [CrossRef] [PubMed]
[9] Tsai, J.P., Lee, C.H., Ying, T.H., et al. (2015) Licochalcone a Induces Autophagy through PI3K/Akt/mTOR Inactivation and Autophagy Suppression Enhances Licochalcone A-Induced Apoptosis of Human Cervical Cancer Cells. Oncotarget, 6, 28851-28866. [Google Scholar] [CrossRef] [PubMed]
[10] 徐燕立, 朱飞叶, 徐珊. “乐胃饮”加味方对慢性萎缩性胃炎模型鼠胃黏膜病变及相关炎症因子的作用[J]. 浙江中医杂志, 2020, 55(2): 93-94.