一种土壤/沉积物中2-甲基苯并噻唑的分析方法

An Analytical Method of 2-Methylbenzothiazole in Soil/Sediment

摘要: 建立了一种土壤中2-甲基苯并噻唑的前处理和定量方法，其主要原理如下：土壤样品经自然风干后，称取10.0 g样品放在玻璃纤维滤膜内，包裹起来，放入索氏提取器中，用100 mL二氯甲烷：丙酮(1:1)索氏抽提18 h，收集提取液，并通过无水硫酸钠除去提取液中的水分，然后用旋转蒸发仪中浓缩至1 mL后，加入过量正己烷(10 mL)，交换溶剂，并再次浓缩至1.0 mL，浓缩液通过内径为1.0 cm填充3.0 g硅胶(100~200目)的层析柱，并加入30 mL正己烷：二氯甲烷(1:1)淋洗，收集淋洗液，浓缩定容至1.0 mL，通过气相色谱–质谱外标法定量。

Abstract: A pretreatment and quantitative method for 2-methylbenzothiazole in soil was established. The main principle is as follows: after natural air drying, place 10.0 g of soil sample in glass fiber filter membrane, wrap and put it into Soxhlet extractor. Soxhlet extraction with 100 mL dichloro-methane:acetone (1:1) for 18 hours, collect the extract, and remove the water in the extract through anhydrous sodium sulfate. Then concentrate to 1 mL with a rotary evaporator, add excess n-hexane (10 mL), exchange the solvent, and concentrate again to 1.0 mL. After the concentrated solution passes through a chromatographic column filled with 3.0 g silica gel (100~200 mesh) with an inner diameter of 1.0 cm, add 30 mL n-hexane:dichloromethane (1:1) for leaching. Collect the leaching solution, concentrate to volume to 1.0 mL, and quantify it by gas chromatography-mass spectrometry external standard method.