去皮苦杏仁粕中苦杏仁苷的提取与鉴定综合实验教学项目创新设计

Innovative Design of Comprehensive Experimental Teaching Project on Extraction and Identification of Amygdalin from Peeled Bitter Almond Meal

摘要: 为了使实验教学跟科研和工业生产相结合，创新设计了从去皮苦杏仁粕中提取苦杏仁苷并进行结构鉴定综合实验教学项目。通过无水乙醇提取去皮苦杏仁粕中的苦杏仁苷，联合运用紫外光谱、高效液相色谱和核磁共振氢谱等现代光谱技术对产品进行结构鉴定，引导学生思考天然产物提取纯化–分子结构–光谱特征三者之间的关系。实验结果表明，该实验不仅有利于激发学生实验兴趣，提高学生动手能力，培养学生创新思维，而且还可以使学生熟悉相关仪器设备的使用，并提高大型仪器设备的使用效率。

Abstract: In order to combine experimental teaching with scientific research and industrial production, a comprehensive experimental teaching project is innovatively designed on extraction and identification of amygdalin from peeled bitter almond meal. Amygdalin is extracted from peeled bitter almond meal by anhydrous ethanol and identified by means of modern spectroscopy technologies such as UV, HPLC and 1H NMR, which can inspire students to think about the relationship between purification, molecular structure and spectral characteristics of natural product. The results show that the experiment is not only conducive to stimulate students’ experimental interest, improve students’ practical ability, cultivate students’ innovative thinking, but also can familiarize students with the use of relevant instruments and equipment, and improve the use efficiency of large instruments and equipment.