不同品牌cf-DNA保存管效果对比
Comparison of Effects of Different Brands of cf-DNA Preservation Tubes
DOI: 10.12677/BP.2021.114016, PDF,   
作者: 李燕虹, 华静宇, 赵安琪, 李 菲, 李丹杰, 程 杰, 崔景强*：河南省医用高分子材料技术与应用重点实验室，河南 长垣；河南驼人医疗器械集团有限公司，河南 长垣
关键词: 采血管；cf-DNA；实时荧光定量PCR；二代测序； Vascular Collection； cf-DNA； q-PCR Quantification； Second Generation Sequencing
摘要: 目的：对比驼人自主研发cf-DNA保存管与市售两种品牌DNA保存管对样本中cf-DNA保存效果。方法：随机选取志愿者，每位志愿者分别静脉采集血液至三种cf-DNA保存管，采集样本分别保存0~2 h，3 d，7 d，10 d，14 d，提取cf-DNA进行实时荧光定量PCR分析及二代测序。结果：在驼人自主研发cf-DNA保存管与市售三种品牌cf-DNA保存管中保存样本测序结果保持一致，驼人自主研发采血管可无损伤稳定保存样本。
Abstract: To compare the effect of self-developed DNA preservation tubes and three brands of commercially available DNA preservation tubes on cf-DNA preservation in samples. Methods: Randomly selected volunteers, blood samples from each volunteer were collected into three kinds of DNA preservation tubes, and the samples were stored for 0~2 h, 3 d, 7 d, 10 d and 14 d respectively. cf-DNA was extracted for q-PCR quantification and sequencing. Results: The sequencing results of samples stored in the DNA preservation tubes independently developed by Camel man were consistent with those of the three brands on the market, and the samples could be stably preserved without damage.
文章引用：李燕虹, 华静宇, 赵安琪, 李菲, 李丹杰, 程杰, 崔景强. 不同品牌cf-DNA保存管效果对比[J]. 生物过程, 2021, 11(4): 140-145. https://doi.org/10.12677/BP.2021.114016

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