基于网络药理学探讨苗药复方龙丹胶囊抗炎的作用机制

To Discuss the Anti-Inflammatory Mechanism of Miao Medicine Compound Longdan Capsules Based on Network Pharmacology

摘要: 目的：探讨苗药复方龙丹胶囊抗炎潜在活性成分和可能的作用机制。方法：通过TCMSP、Swiss Target Prediction、Uniprot等数据库并结合文献挖掘苗药复方龙丹胶囊的活性成分和作用靶点，运用OMIM、Genecards生物数据库筛选出炎症的相关靶点，取两者交集。将交集靶点导入STRING数据库获得.tsv文件，导入cytoscape软件，绘制PPI网络及筛选核心靶点。将核心靶点导入David数据库进行基因本体(GO)功能、京都基因和基因百科全书(KEGG)通路富集分析。结果：筛选出苗药复方龙丹胶囊117个活性成分和439个潜在靶点，10876个炎症疾病相关靶点，药物与疾病共有397个交集靶点。苗药复方龙丹胶囊可能通过调节TP53、EGFR、PIK3R1、PIK3CA、RB1等靶点，干预PI3K-Akt通路、HIF-1通路、癌症通路等相关信号通路，从而达到抗炎的作用。结论：该研究通过网络药理学方式预测了苗药复方龙丹胶囊抗炎的主要活性成分、靶点和相关通路，可为进一步研究提供参考。

Abstract: Objective: To study the potential anti-inflammatory ingredients and possible mechanism of Miao Medicine Compound Longdan Capsules. Methods: The TCMSP, Swiss Target Prediction, Uniprot and other databases were used to excavate the active ingredients and targets of Miao Medicine Compound Longdan Capsules combining with literature. Using OMIM and Genecards biological database to screen out related targets of inflammation, and take the intersection of the two. Importing the intersection target into the STRING database to obtain the .tsv file and enter the cytoscape software, and then draw the PPI network and screen the core targets. In addition, the core targets were imported into the David database for gene ontology (GO) function, Kyoto Gene and Encyclopedia of Genes (KEGG) pathway enrichment analysis. Results: 117 active ingredients and 439 potential targets of Miao Medicine Compound Longdan Capsules were screened, 10876 disease-related targets, and a total of 397 intersection targets for drugs and diseases. The Compound Longdan Capsule may regulate TP53, EGFR, PIK3R1, PIK3CA, RB1 and other targets, and interfere with PI3K-Akt pathway, HIF-1 pathway, cancer pathway and other related signal pathways to achieve anti-inflammatory effects. Conclusion: This study predicts the main active ingredients, targets and related pathways of Compound Longdan Capsule’s anti-inflammation through network pharmacology, which can provide reference for further research.