栝楼桂枝汤对脑缺血/再灌注大鼠脑组织葡萄糖代谢的作用及机制研究
Effects of Gualou Guizhi Decoction on Glucose Metabolism in Rats with Cerebral Ischemia/Reperfusion and Its Mechanisms
DOI: 10.12677/TCM.2022.111016, PDF,    国家自然科学基金支持
作者: 韩 静, 张继州, 邹 俊, 蒋 畅：福建省中医药科学院，福建 福州
关键词: 栝楼桂枝汤；脑缺血；Micro-PET；葡萄糖转运体-1；Gualou Guizhi Decoction； Cerebral Ischemia； Micro-PET； Glut-1
摘要: 目的：动态观察栝楼桂枝汤对脑缺血再灌注损伤后脑组织葡萄糖代谢的作用及葡萄糖转运体相关的机制。方法：SD大鼠随机分为假手术组、模型组和栝楼桂枝汤组，采用线栓法制作大脑中动脉闭塞再灌注模型，栝楼桂枝汤组于造模后连续14日灌胃栝楼桂枝汤予以干预，每日1次。分别于造模后3天、7天和14天，采用Micro-PET活体影像检测方法，观察大鼠脑组织造影剂18F-FDG摄取情况变化。采用免疫组织化学方法检测脑缺血周边区葡萄糖转运体Glut-1的表达。结果：随着用药时间增加，栝楼桂枝汤可显著降低缺血损伤大鼠葡萄糖低摄取区域(p < 0.05)。免疫组织化学结果显示栝楼桂枝汤组Glut-1阳性染色较模型组显著提高(p < 0.05)。结论：栝楼桂枝汤可促进脑缺血损伤大鼠脑组织的葡萄糖摄取，该作用可能与其提高脑组织葡萄糖转运相关。
Abstract: The purpose of this article is to observe the effects of Gualou Guizhi Decoction on the glucose metabolism of brain tissue after cerebral ischemia-reperfusion injury and the underlying mechanism related to glucose transporter. SD rats were randomly divided into sham operation group, model group and Gualou Guizhi Decoction group. The middle cerebral artery occlusion and reperfusion surgery were performed by plugging a thread into the artery. Following the surgery, the rats in the Gualou Guizhi Decoction group were given the compound once daily for 14 consecutive days. While rats in the sham group and model group were administrated vehicle. At 3, 7 and 14 days after modeling, in vivo Micro-PET imaging was performed to observe the uptake of 18F-FDG in the brain tissue of rats. Then immunohistochemical method was used to detect the expression of glucose transporter (Glut-1) in the peripheral area of ischemia. Results showed that, with the increase of medication time, Gualou Guizhi Decoction significantly reduced the volume of low uptake area of glucose in rats with ischemic injury (p < 0.05). Immunohistochemistry staining showed that the Glut-1 positive staining in the Gualou Guizhi Decoction group was significantly higher than that in the model group (p < 0.05). The results indicate that Gualou Guizhi Decoction promotes brain glucose uptake in rats with cerebral ischemia injury. This effect may be related to the compound’s ability to improve the Glut-1 of brain tissue.
文章引用：韩静, 张继州, 邹俊, 蒋畅. 栝楼桂枝汤对脑缺血/再灌注大鼠脑组织葡萄糖代谢的作用及机制研究[J]. 中医学, 2022, 11(1): 89-95. https://doi.org/10.12677/TCM.2022.111016

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