芭蕉、香蕉及野蕉花及苞片中羽扇豆酮的分离、鉴定及含量测定
The Isolation, Identification and Content Determination of Lupenone in the Flowers and Bracts of Musa basjoo, Musa paradisiacal, Wild Musa basjoo
DOI: 10.12677/AAC.2022.121001, PDF,    国家自然科学基金支持
作者: 余 漂, 丁 芹, 王远敏, 梁玉清, 吴红梅*：贵州中医药大学，贵州 贵阳；王祥培：贵州民族大学，贵州 贵阳
关键词: 芭蕉；香蕉；野蕉；花；苞片；羽扇豆酮；含量测定；Musa basjoo； Musa paradisiacal； Wild Musa basjoo； Flowers； Bracts； Lupenone； Content Determination
摘要: 目的：对芭蕉、香蕉及野蕉的花和苞片中羽扇豆酮进行分离与结构鉴定，并建立其含量测定方法。方法：利用硅胶柱色谱、质谱和核磁共振对芭蕉、香蕉及野蕉的花和苞片中的化合物进行分离纯化及结构鉴定；以高效液相色谱(HPLC)法对其含量进行测定。结果：从芭蕉、香蕉及野蕉的花和苞片中都能分离出羽扇豆酮；香蕉和野蕉的花中羽扇豆酮平均含量明显高于芭蕉花(P < 0.05)，芭蕉苞片中羽扇豆酮平均含量明显高于香蕉和野蕉的苞片(P < 0.05)；同植株芭蕉老花和嫩花中以老花中羽扇豆酮含量最高，芭蕉老苞片和嫩苞片中以老苞片的含量最高，三个品种中同植株花和苞片中羽扇豆酮含量均以苞片的较高。结论：本研究建立的含量测定方法简便、快捷、准确性高、重复性及专属性强，可用于不同品种香蕉、芭蕉及野蕉的花和苞片中羽扇豆酮的含量测定。提示芭蕉、香蕉及野蕉的花及苞片可作为抗糖尿病活性成分羽扇豆酮原药材来源。
Abstract: Objective: To isolate and identify the lupenone in the flowers and bracts of Musa basjoo, Musa paradisiacal, Wild Musa basjoo, and establish a method to determine the contents. Method: The isolation and chemical structure identification of lupenone contained in the flowers and bracts of M. basjoo, M. paradisiacal, Wild M. basjoo were performed by silica gel column chromatography, mass spectrum and nuclear magnetic resonance; HPLC method was adapted to detect the contents of lupenone in the flowers and bracts of M. basjoo, M. paradisiacal, Wild M. basjoo. Result: The lupenone was isolated from the flowers and bracts of M. paradisiacal, M. basjoo, Wild M. basjoo. The average contents of lupenone in the flowers of M. paradisiacal and Wild M. basjoo were significantly higher than M. basjoo (P < 0.05), and the average contents of lupenone in the bracts of M. basjoo were significantly higher than M. paradisiacal and Wild M. basjoo (P < 0.05); the lupenone content in the old flowers was higher than that in the tender flowers of M. basjoo from same plant, and the lupenone content in the old bracts was higher than that in the tender bracts of M. basjoo from same plant. The lupenone content in bracts of M. paradisiacal, M. basjoo, Wild M. basjoo was higher than that in bracts of the same plant. Conclusion: The assay method established in this study is simple, rapid, accurate, repeatable and specific. It can be used for the content determination of lupenone in the flowers and bracts of M. basjoo, M. paradisiacal, Wild M. basjoo.
文章引用：余漂, 丁芹, 王祥培, 王远敏, 梁玉清, 吴红梅. 芭蕉、香蕉及野蕉花及苞片中羽扇豆酮的分离、鉴定及含量测定[J]. 分析化学进展, 2022, 12(1): 1-12. https://doi.org/10.12677/AAC.2022.121001

参考文献

[1] 国家中医药管理局《中华本草》编委会. 中华本草·苗药卷[M]. 贵阳: 贵州科技出版社, 2005: 292.
[2] 贵州省药品监督管理局. 贵州省中药材、民族药材质量标准[M]. 贵阳: 贵州科技出版社, 2003: 346.
[3] 盛占武, 王会, 郑丽丽, 等. 香蕉花提取物中抑制α-葡萄糖苷酶活性组分研究[J]. 中国食品学报, 2014, 14(9): 68-74.
[4] 钱海兵, 孙宜春, 黄婕, 等. 芭蕉根不同提取物的抗炎镇痛作用研究[J]. 时珍国医国药, 2010, 21(4): 780-781.
[5] 钱海兵, 郝俊杰, 王祥培. 芭蕉根有效部位对小鼠血糖及糖耐量的影响[J]. 中国实验方剂学杂志, 2012, 18(18): 187-189.
[6] 张家荣. 滇南芭蕉饮食文化[J]. 西部大开发, 2006(8): 49-50.
[7] 周玲. 药用食疗芭蕉花[J]. 花木盆景(花卉园艺), 2003(9): 42.
[8] 李伟良. 傣族地区芭蕉类植物的民族植物学研究[J]. 中国野生植物资源, 2018, 37(4): 54-59.
[9] 刘洋, 卢群, 周志远, 等. 芭蕉植物的研究及开发进展[J]. 广东药学院学报, 2013, 29(6): 675-677+681.
[10] 刘春荣, 何国栋, 肖小青. 野芭蕉花液对糖尿病模型大鼠血糖、血脂的影响[J]. 时珍国医国药, 2013, 24(2): 355-356.
[11] 王祥培, 郝俊杰, 许士娜, 等. 芭蕉根醋酸乙酯部位的化学成分研究[J]. 时珍国医国药, 2012, 23(3): 515-516.
[12] Xu, F., Wu, H.M., Wang, X.P., et al. (2014) RP-HPLC Characterization of Lupenone and β-Sitosterol in Rhizoma Musae and Evaluation of the Anti-Diabetic Activity of Lupenone in Diabetic Sprague-Dawley Rats. Molecules, 19, 14114-14127.
[Google Scholar] [CrossRef] [PubMed]
[13] Xu, F., Yang, L.B., Huang, X.L., et al. (2020) Lupenone Is a Good Anti-Inflammatory Compound Based on the Network Pharmacology. Molecular Diversity, 24, 21-30.
[Google Scholar] [CrossRef] [PubMed]
[14] 吴红梅, 徐锋, 王远敏, 等. 羽扇豆酮对2型糖尿病大鼠胰岛素抵抗的一般情况、葡萄糖耐量及胰岛素耐量的影响[J]. 时珍国医国药, 2017, 28(5): 1035-1037.
[15] Chiang, S.H., Yang, K.M., Lai, Y.C., et al. (2020) Evaluation of the in Vitro Biological Activities of Banana Flower and Bract Extracts and Their Bioactive Compounds. International Journal of Food Properties, 24, 1-16.
[Google Scholar] [CrossRef] 
[16] Ganugapati, J., Baldwa, A. and Lalani, S. (2012) Molecular Docking Studies of banana Flower Flavonoids as Insulin receptor Tyrosine Kinase Activators as a Cure for Diabetes Mellitus. Bioinformation, 8, 216-220.
[Google Scholar] [CrossRef] [PubMed]
[17] Ahn, E.K. and Oh, J.S. (2013) Lupenone Isolated from Adenophora triphylla var. japonica Extract Inhibits Adipogenic Differentiation through the Down Regulation of PPARγ in 3T3-L1 Cells. Phytotherapy Research, 27, 761-766.
[Google Scholar] [CrossRef] [PubMed]
[18] 李小芬, 吴红梅, 王祥培. UPLC法测定香蕉、皇帝蕉皮及其果肉中羽扇豆酮含量[J]. 食品科学, 2017, 38(22): 156-161.
[19] 李小芬, 王远敏, 王祥培, 等. HPLC法测定5个采收期香蕉、野蕉、皇帝蕉果皮中羽扇豆酮[J]. 中成药, 2017, 39(12): 2630-2632.
[20] 吴红梅, 孔娟, 黄旭龙, 等. UPLC法测定芭蕉药材不同部位中羽扇豆酮和豆甾醇的含量[J]. 中国药房, 2021, 32(5): 542-546.
[21] 马博, 苏仕林, 黄娇丽. 野生芭蕉花与假茎的营养成分分析[J]. 食品工业, 2018, 39(6): 313-316.
[22] 包艳玲, 高春燕, 卢跃红. 四种野生蔬菜营养成分分析[J]. 食品工业科技, 2021, 42(7): 337-341.
[23] 张倩. 芭蕉根、芭蕉花和南湖菱活性成分研究[D]: [硕士学位论文]. 开封: 河南大学, 2011.
[24] Tai, Z., Chen, A., Qin, B., et al. (2014) Chemical Constituents and Antioxidant Activity of the Musa basjoo Flower. European Food Research and Technology, 239, 501-508.
[Google Scholar] [CrossRef] 
[25] 方紫岑, 周志远, 谢哲, 等. 芭蕉花活性成分提取及其体外生物活性研究[J]. 广东药科大学学报, 2017, 33(4): 503-508.
[26] Mathew, N.S. and Negi, P.S. (2017) Traditional Uses, Phytochemistry and Pharmacology of Wild Banana (Musa acuminata Colla): A Review. Journal of Ethnopharmacology, 196, 124-140.
[Google Scholar] [CrossRef] [PubMed]

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