没食子酸在烟曲霉菌诱导的真菌性角膜炎中的抗炎作用

Anti-Inflammatory Effects of Gallic Acid against Fungal Keratitis Induced by Aspergillus fumigatus

摘要: 没食子酸(gallic acid, GA)具有抗炎、抗氧化、抑菌、抗病毒、抗肿瘤、心血管保护等多种生物活性，并且广泛用于医药、食品等领域。本文就GA对烟曲霉菌菌丝诱导的人角膜上皮细胞炎症反应及对小鼠真菌性角膜炎的治疗作用进行相关研究。方法：体外实验用烟曲霉菌菌丝感染人角膜上皮细胞后，再用GA处理，PCR、ELISA方法检测IL-1β、TNF-α、LOX-1的mRNA及蛋白的表达。体内实验用烟曲霉菌孢子感染C57BL/6小鼠角膜建立烟曲霉菌角膜炎模型，然后使用GA或PBS处理，比较两组的临床表现并进行炎症评分以比较两者差异。结果：GA降低了烟曲霉菌诱导的人角膜上皮的炎症反应中的IL-1β、TNF-α、LOX-1的表达，且改善真菌性角膜炎小鼠角膜的炎症程度。结论：GA抑制真菌菌丝诱导的人角膜上皮的炎症因子表达，改善真菌性角膜炎小鼠角膜的炎症程度评分。

Abstract: Gallic acid (GA) has various biological activities such as anti-inflammatory, antioxidant, bacteriostatic, anti-viral, anti-tumor, cardiovascular protection, etc., and is widely used in medicine, food and other fields. In our study, we focus on the therapeutic effect of GA on the inflammatory response of human corneal epithelial cells induced by Aspergillus fumigatus hyphae and on the mice fungal keratitis. Methods: In vitro experiments, human corneal epithelial cells were infected by Aspergillus fumigatus hyphae and treated with GA, the expression of IL-1β, TNF-α and LOX-1 was detected by PCR and ELISA. In vivo experiments, Aspergillus fumigatus keratitis model was made by infected the cornea of C57BL/6 mice with Aspergillus fumigatus, and then treated with GA or PBS. The clinical manifestations of the two groups were compared and the clinical score was marked to quantify the differences. Results: GA reduced the expression of IL-1β, TNF-α and LOX-1 in the inflammatory response of human corneal epithelium induced by Aspergillus fumigatus and improved the degree of inflammation of the cornea in mice with fungal keratitis. Conclusion: GA inhibited the expression of inflammatory factors in the human corneal epithelium induced by fungal hyphae and improved the inflammation degree of fungal keratitis mice cornea.