蒙药蓝盆花HPLC指纹图谱及主要成分的含量测定研究

HPLC Fingerprint and Determination of Main Components of Mongolian Medicine of the Flos of Scabiosa comosa Fisch

摘要: 目的：对不同产地蒙药材蓝盆花(含蒙古国)进行质量比较研究，为该药质量标准制定提供科学依据，确保临床疗效及复方制剂用药安全提供依据。方法：采用HPLC进行测定，色谱柱为Welchrom-C₁₈ (4.6 mm × 300, 5 µm)，流动相为乙腈-0.1%磷酸水，梯度洗脱，流速为1 mL/min，检测波长为254 nm，柱温40℃，进样量为10 µl，运用中药色谱指纹图谱相似度评价系统A版色谱软件对指纹图谱进行相似度评价。结果：建立了不同产地蓝盆花的HPLC指纹图谱，确定了22个共有峰，指认了9个共有峰，计算了相似度，相似度在0.907~0.996。结论：建立了蒙药蓝盆花药材的HPLC特征指纹图谱，该指纹图谱测定方法精密度、稳定性和重复性良好。

Abstract: Objective: HPLC method was used to study the fingerprint of Mongolian medicine of the Flos of Scabiosa comosa Fisch in different collection areas, and the content of effective components was determined. The quality of Mongolian medicine blue potted flower was evaluated by the similarity evaluation system of spectral fingerprint. Methods: HPLC was used for determination. The chromatographic column was Welchrom-c₁₈ (4.6 mm × 300, 5 µm), the mobile phase was acetonitrile-0.1% phosphoric acid water, gradient elution was adopted, the flow rate was 1 mL/min, the detection wavelength was 254 nm, the column temperature was 40℃, and the injection volume was 10 µΙ. Results: HPLC fingerprints of Mongolian medicine of the Flos of Scabiosa comosa Fisch from different habitats were established, 22 common peaks and 9 common peaks were identified, and the similarity was calculated, ranging from 0.907~0.996. Conclusion: The HPLC fingerprint of Mongolian medicine of the Flos of Scabiosa comosa Fisch was further established, and the established method was accurate, stable and reproducible.