担子菌Mating-Type序列生物信息学分析
Bioinformatics Analysis of Mating-Type Sequence of Basidiomycetes
DOI: 10.12677/AMB.2022.111003, PDF,   
作者: 杜琳霖, 吴红梅, 唐光甫, 满海乔, 赵杰宏*：贵州中医药大学，贵州 贵阳；王祥培：贵州民族大学，贵州 贵阳
关键词: 草菇；担子菌；交配型；异宗配合；生物信息学；Volvariella volvacea； Basidiomycotina； Mating-Type； Heterothallism； Bioinformatics
摘要: 目的：以草菇为研究对象，对担子菌mating-type同源序列中的HD位点为靶标进行生物信息学初步分析。方法：应用在线生物信息学软件预测HD序列的理化性质、疏水性/亲水性、导肽、跨膜结构、三维结构、功能结构域，并构建了同亚门中其他物种该蛋白家族的系统进化树。结果：同源mating-type序列中HD基因位点的氨基酸不稳定性指数在56%左右，且无明显的信号肽、导肽和跨膜结构域，三维结构由3个α-螺旋和少数转角二级结构组成，七种食用菌在氨基酸序列呈现较低的相似性。结论：同源mating-type序列的HD1、HD2蛋白稳定性差，无信号肽、导肽、跨膜结构，无β-折叠，在N-J进化树中不同物种间该氨基酸序列相似性极低，表明HD位点具有丰富的多样性，且结构性简单。
Abstract: Objective: The objective is to take Volvariella volvacea as the research object, and conduct a preliminary bioinformatics analysis on the HD site in the mating-type homologous sequence of Basidiomycetes. Methods: Using online bioinformatics software to predict the physical and chemical properties, hydrophobicity/hydrophilicity, guide peptide, transmembrane structure, three-dimensional structure, and functional domains of HD sequences, and construct a system of this protein family of other species in the same subphylum evolutionary tree. Results: The amino acid instability index of the HD locus in the homologous mating-type sequence is about 56%, and there is no obvious signal peptide, guide peptide, and transmembrane domain. The three-dimensional structure consists of 3 α-helices and a few turns in terms of secondary structure, and the seven edible fungi show low similarity in amino acid sequence. Conclusion: The HD1 and HD2 proteins with homologous mating-type sequences have poor stability, no signal peptide, guide peptide, transmembrane structure, and no β-sheet. The amino acid sequence similarity between different species in the N-J evolutionary tree is extremely low, indicating that HD sites are rich in diversity and simple in structure.
文章引用：杜琳霖, 吴红梅, 王祥培, 唐光甫, 满海乔, 赵杰宏. 担子菌Mating-Type序列生物信息学分析[J]. 微生物前沿, 2022, 11(1): 22-30. https://doi.org/10.12677/AMB.2022.111003

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