转基因苜蓿J163品系定性标准样品的研制

Development of Qualitative Reference Material of Genetically Modified Alfalfa Event J163

摘要: 研究目的：研制转基因苜蓿J163品系的基体定性标准样品，从而提高转基因苜蓿J163品系检测的科学性和一致性。方法：将经PCR方法验证的转基因J163品系苜蓿种子作为候选物，通过研磨、过筛、搅拌、冷冻抽干制备得到定性标准样品，分装后进行均匀性和稳定性检验，并联合8家外部实验室对标准样品进行了定值。结果：经8家权威实验室协同定值验证，PCR检测的结果判定均为转基因苜蓿J163品系阳性，与研制单位的检测结果相一致。结论：制备的标准样品均匀性和稳定性均达到了国家标准样品的技术要求，可用于转基因苜蓿J163品系分子检测技术的质量控制、检测试剂的验证和评价。

Abstract: Aim: The matrix qualitative reference material of genetically modified alfalfa event J163 was de-veloped to improve the scientificity and consistency of J163 detection. Method: The J163 alfalfa seeds verified by PCR method were used as candidates, and the qualitative reference material was prepared by grinding, screening, stirring and freeze-drying. After sub packaging, the uniformity and stability were tested, and the reference material was evaluated in conjunction with 8 external laboratories. Results: The results of PCR verified by 8 authoritative laboratories were all positive for genetically modified alfalfa event J163, which was consistent with the test results of the development laboratory. Conclusion: The uniformity and stability of the prepared reference material meet the technical requirements of the national reference material, and can be used for the quality control of molecular detection technology of genetically modified alfalfa event J163 and the verification and evaluation of detection reagents.