原发性胆汁性胆管炎患者血清MicroRNA的表达谱分析

Analysis of Serum MicroRNA Expression Profile in Patients with Primary Biliary Cholangitis

摘要: 目的：研究原发性胆汁性胆管炎(Primary biliary cholangitis, PBC)患者血清中microRNAs的差异表达与临床价值。方法：首先，采用高通量测序筛选PBC组和正常对照组中差异表达的miRNAs，其次，采用实时荧光定量PCR (qRT-PCR)对差异表达的miRNAs在扩大样本中进行验证。最后，预测miRNAs的靶基因。结果：高通量测序筛选出94条miRNAs，其中，60条miRNAs表达上调，34条miRNAs表达下调。经过qRT-PCR验证发现miR-410-3p在PBC组中的表达水平明显高于正常对照组，与测序结果一致。靶基因预测结果提示，miR-410-3p的相关靶基因与T细胞或B细胞的分化、活化、增殖、稳态相关。结论：PBC患者血清中存在差异表达的miRNAs，miR-410-3p对PBC的诊断有一定的参考价值。

Abstract: Objective: To investigate the differential expression and clinical value of microRNAs in the serum of patients with primary biliary cholangitis (PBC). Methods: First, high-throughput sequencing was used to screen differentially expressed miRNAs in PBC group and normal control group. Second, qRT-PCR was used to verify the differentially expressed miRNAs in expanded samples. Finally, computer analysis was conducted to predict target genes. Results: By high-throughput sequencing, we found 94 miRNAs were differentially expressed in PBC patients compared to the normal controls. The analysis identified 60 up-regulated miRNAs (>2.0-fold) and 34 down-regulated miRNAs (<0.5-fold). qRT-PCR verified that the expression of miR-410-3p was significantly up-regulated in PBC patients compared with the normal controls, which was consistent with the deep sequencing results. The prediction results of target genes suggested that the related target genes of miR-410-3p were involved in the differentiation, activation, proliferation and homeostasis of T cells or B cells. Conclusion: There are differentially expressed miRNAs in the serum of PBC patients. miR-410-3p has certain reference value for the diagnosis of PBC.