小鼠低剂量脾区照射与低剂量全身照射对T细胞免疫功能的影响

Effects of Low Dose Splenic Region Irradiation and Low Dose Total Body Irradiation on T Cell Immune Function in Mice

摘要: 目的：通过观察小鼠低剂量脾脏照射、低剂量全身照射与空白对照组小鼠脾脏指数、脾脏T淋巴细胞亚群变化，探讨其差异变化的意义。方法：经不同辐射剂量照射小鼠脾区或全身后，建立小鼠模型。处死小鼠取脾脏组织计算各组小鼠脾脏指数改变、采用流式细胞术分析脾脏T淋巴细胞亚群改变。结果：与空白对照组相比，低剂量脾区照射组与低剂量全身照射组小鼠脾脏指数均升高，而低剂量脾区照射组小鼠升高更为明显；低剂量脾区照射组、低剂量全身照射组小鼠脾脏CD3+ T淋巴细胞、CD4+ T淋巴细胞较空白对照组比例升高，低剂量脾区照射组升高更为明显。结论：经低低剂量脾区照射、低剂量全身照射小鼠后，脾脏T淋巴细胞亚群发生改变，使CD3+ T淋巴细胞、CD4+ T淋巴细胞表达比例升高，而低剂量脾区照射刺激作用更强。

Abstract: Objective: To observe the changes of spleen index and T lymphocyte subsets in mice with low dose spleen irradiation, low dose total body irradiation and blank control group, and to explore the significance of the difference. Method: The mouse model was established by irradiating the spleen or the whole body with different doses of radiation. The spleen tissues of the mice were taken to calculate the changes of spleen index and the changes of T lymphocyte subsets in spleen were analyzed by flow cytometry. Result: Compared with blank control group, spleen index of mice in low dose splenic area irradiation group and low dose total body irradiation group increased, but the increase of mice in low dose splenic area irradiation group was more obvious. Compared with blank control group, the proportion of CD3+ T lymphocytes and CD4+ T lymphocytes in spleen of mice in low dose splenic area irradiation group and low dose total body irradiation group increased, and the increase was more obvious in low dose splenic area irradiation group. Conclusion: The spleen T lymphocyte subsets of mice were changed after low and low dose splenic irradiation and low dose total body irradiation, and the expression proportion of CD3+ T lymphocyte and CD4+ T lymphocyte increased, while the stimulation effect of low dose splenic irradiation was stronger.