微嗜酸寡养单胞菌4-1对Cr(VI)污染土壤的修复研究

Remediation of Cr(VI)-Contaminated Soil by Stenotrophomonas acidaminiphila 4-1

摘要: 六价铬是铬残留物中的主要有害物质，铬污染场地的修复一直是重金属污染控制领域的重要研究课题。生物修复技术是一种用于处理铬污染场地的环保技术。本研究利用从胜利油田油污土壤中分离出一株可耐受高浓度六价铬[Cr(VI)]抗性菌株，进行16S rDNA测序和数据库比对后，将其命名为微嗜酸寡养单胞菌(Stenotrophomonas acidaminiphila) 4-1。针对初始Cr(VI)浓度、菌液接种量、土壤含水率和葡萄糖添加量等因素对土壤环境中Cr(VI)去除率的影响进行了研究。并采用响应曲面法对上述因素的交互影响进行了优化，得出在土壤含水率为28.6%、菌液接种量为4.53 mL以及葡萄糖添加量为5.97%时，Cr(VI)最佳去除率为85.0%。研究结果表明微嗜酸寡养单胞菌4-1是铬污染场地生物修复的优秀菌源。

Abstract: Hexavalent chromium is the main harmful substance in chromium residues, and the remediation of chromium-contaminated sites has always been an important research topic in the field of heavy metal pollution control. Bioremediation is an environmentally friendly technology for treating chromium-contaminated sites. In this study, a high-concentration hexavalent chromium [Cr(VI)]-resistant strain was isolated from oily soil in Shengli Oilfield, and named after 16S rDNA sequencing and database comparison, Stenotrophomonas acidaminiphila 4-1. The effects of initial Cr(VI) concentration, bacterial inoculum amount, soil moisture content and glucose addition on the removal rate of Cr(VI) in soil were studied. The interaction of the above factors was optimized by the response surface method, and it was concluded that when the soil water content was 28.6%, the bacterial solution inoculum was 4.53 mL, and the glucose addition was 5.97%, the optimal removal rate of Cr(VI) was as follows: 85.0%. The results showed that Stegotrophomonas acidaminiphila 4-1 was an excellent bacterial source for the bioremediation of chromium-contaminated sites.