左旋多巴表面接枝溶菌酶构建抗菌涂层的研究

Construction of Antibacterial Coating by Grafting Lysozyme onto Levodopa Surface

摘要: 医疗器械在服役过程中可能引发感染等并发症，从而诱发更严重的不良事件，严重危害患者的生命安全，增加医疗成本。目前临床上通常使用抗生素来治疗或预防器械表面诱发感染，然而传统抗生素面临着过敏及耐药等风险。因此通过赋予医疗器械表面抗菌功能对于降低器械诱导的不良事件发生具有重要意义。我们报导了一种基于贻贝黏附灵感以及共价固定具有抗菌功能活性分子的抗菌表面构建策略。首先使用左旋多巴改性基底材料，构建具有二次反应位点的多官能团表面，为生物活性分子的共价固定提供了平台。然后采用碳二亚胺化学成功在其表面共价固定具有抗菌生物活性的溶菌酶分子，通过材料学表征证明了涂层的成功制备并研究了涂层的基本性质及表面化学组成，通过功能性验证证明了溶菌酶涂层具有良好的抗菌能力。这为医疗器械抗菌表面涂层构建提供了新的方法，同时也为生物活性分子的共价固定提供了新的思路。

Abstract: During the service of medical devices, complications such as infection may occur, which will induce more serious adverse events. Seriously endanger the life safety of patients, and increase medical costs. Currently, antibiotics are commonly used in clinical practice to treat or prevent infections induced on the surface of devices. However, traditional antibiotics face risks such as allergy and drug resistance. Therefore, it is of great significance to reduce the occurrence of device-induced adverse events by imparting antibacterial function to the surface of medical devices. We report an antibacterial surface construction strategy inspired by mussel adhesion and covalently immobilizing active molecules with antibacterial functions. First, the substrate material was modified with levodopa to construct a multifunctional surface with secondary reaction sites, which provided a platform for the covalent immobilization of bioactive molecules. Then, carbodiimide chemistry was used to successfully covalently immobilize lysozyme molecules with antibacterial biological activity on its surface. The successful preparation of the coating was proved by material characterization, and the basic properties and surface chemical composition of the coating were studied. The validation proved that the lysozyme coating has good antibacterial ability. This provides a new method for the construction of antibacterial surface coatings for medical devices, and also provides a new idea for the covalent immobilization of bioactive molecules.