基于酚胺化学构建生物抗污涂层的研究

Phenolamine Chemistry for the Construction of Biofouling Resistant Coatings

摘要: 血液接触类器械，如体外膜肺氧合设备(ECMO)，中心静脉导管(CVC)及心脏起搏器(PM)等，在救治肺功能衰竭危重病人、持续性给药及心脏功能维持等方面发挥了重大作用，然而这些器械在使用过程中可能会诱发血小板黏附从而引起血栓等严重问题，因此在生物材料表面构建具有优异抗污性能的涂层尤为重要。本文基于多巴胺(DA)邻苯二酚结构的黏附能力，以及邻苯二酚结构与己二胺(HD)的酚胺化学，将两种分子共价交联，通过一步分子/离子自组装在材料表面构建富胺基DA/HD涂层。以DA/HD涂层表面伯胺基为反应位点，通过碳二亚胺化学接枝牛血清白蛋白(BSA)，成功构建BSA生物抗污涂层。利用BSA的抗粘附性能，在血液接触材料表面构建生物抗污涂层。

Abstract: Blood contact devices such as extracorporeal membrane oxygenation (ECMO) devices, central ve-nous catheters (CVC) and pacemakers (PM) play a major role in the treatment of severe patients with lung failure, continuous drug delivery and cardiac maintenance, but the use of these devices can induce platelet adhesion and lead to serious problems such as thrombosis. Therefore, it is particularly important to build coatings with excellent fouling resistance on the surface of biological materials. In this paper, based on the adhesion ability of the catechol structure of the dopamine (DA) molecule and the phenolamine chemistry of the catechol structure with hexanediamine (HD), the two molecules were covalently cross-linked to construct an amino-rich adhesive DA/HD coating on the surface of the material by one-step molecular/ionic self-assembly. The BSA biofouling coating was successfully constructed by chemical grafting of bovine serum albumin (BSA) molecules with carbodiimide using primary amino groups on the surface of the DA/HD coating as the reactive site. The anti-adhesive properties of BSA are used to construct biofouling coatings on the surface of blood contact materials.