MMP11基因对卵巢癌细胞增殖能力的影响
A Novel Role of MMP11 Responsible for Proliferation of Ovarian Cancer Cells
DOI: 10.12677/WJCR.2022.122008, PDF,    科研立项经费支持
作者: 赵 强：天津市肿瘤医院空港医院，肿瘤精准检测与转化中心，天津；李兴广*：中国科学院大学宁波华美医院，浙江 宁波；国科宁波生命与健康产业研究院，浙江 宁波
关键词: 卵巢癌；MMP11；增殖；生物标记物；Ovarian Cancer； MMP11； Proliferation； Biomarker
摘要: 目的：分析MMP11基因在卵巢癌细胞中的表达特征及对细胞增殖的影响。方法：运用实时荧光定量PCR (qRT-PCR)和Western blotting分别在RNA和蛋白质水平上检测卵巢癌临床样本和卵巢癌细胞系(COV504)中MMP11基因的表达，并用CCK8实验研究MMP11对细胞增殖能力的影响。结果：在卵巢癌临床样本和细胞系中，MMP11基因的表达明显上调，且对卵巢癌细胞的增殖有促进作用。结论：MMP11与卵巢癌起始和发展存在重要关系，且有可能成为卵巢癌的诊断和治疗相关的有效的生物标记物。
Abstract: Objective: To define the MMP11 expression characteristics and analyse the effect of MMP11 on the proliferation of ovarian cancer cells. Method: qRT-PCR and Western blotting were performed to analyse MMP11 expression level in clinical tissues and cell lines of ovarian cancer (COV504), either on RNA level, or on protein level. Additionally, CCK8 assay was validated to assess the influence of MMP11 on the proliferation of ovarian cancer cells. Result: The expression of MMP11 was up-regulated in ovarian cancer and MMP11 could promote the proliferation of ovarian cancer cells. Conclusion: MMP11 was positively associated with the initiation and progression of ovarian cancer, subsequently rendering it as potentially effective biomarker for the early diagnosis and treatment of ovarian cancer.
文章引用：赵强, 李兴广. MMP11基因对卵巢癌细胞增殖能力的影响[J]. 世界肿瘤研究, 2022, 12(2): 61-66. https://doi.org/10.12677/WJCR.2022.122008

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