软枣猕猴桃种苗离体快繁

Soft Jujube Kiwi Fruit Seedlings in Vitro and Rapid Propagation

摘要: 为优化软枣猕猴桃种苗快繁技术体系，以其茎段、芽作为外植体，开展不同激素配比组合的MS培养基对愈伤组织诱导及增殖、不定芽分化增殖、无菌苗生根和驯化移栽等实验研究。结果表明，茎段外植体表面消毒时长6~7 min，在MS + 0.2 mg/L 2,4-D + 0.4 mg/L 6-BA + 蔗糖30 g/L + 琼脂粉7.8 g/L培养基上愈伤组织诱导率最高，达93.75%，且愈伤组织生长旺盛，呈白绿色湿润疏松状；在MS + 1.3 mg/L 6-BA + 0.25 mg/L NAA软枣猕猴桃初代培养芽苗诱导率、增殖率最高，达到93.75%，在MS + 2.0 mg/L 6-BA + 1.2 mg/L NAA培养基上增殖效率最高，达到86.6%，增殖系数达到5左右；最佳生根培养基为1/2MS + 1.5 mg/L NAA，生根率93.75%,并且每株有4~10条根，根粗苗壮；根长3.0～5.0 cm的再生植株移栽到草炭: 园土: 珍珠岩 = 2:2:1的混合基质中，成活率达86%以上。

Abstract: In order to optimize the rapid propagation technology system of soft jujube kiwi fruit seedlings, the stem segment and bud were used as explants to carry out experimental studies on callus induction and proliferation, adventitious bud differentiation and proliferation, roots and acclimation and transplanting of sterile seedlings on MS medium with different hormone ratios. The results showed that the callus induction rate reached 93.75% on MS medium with 0.2 mg/L 2,4-D + 0.4 mg/L 6-BA + sucrose 30 g/L + AGAR powder 7.8 g/L after surface disinfection for 6~7 min, and the callus growth was very strong and showed white green moist loose shape; The induction rate and proliferation rate of kiwi soft jujube in MS + 1.3 mg /L 6-BA + 0.25 mg/L NAA reached 93.75%, and the proliferation rate of kiwi soft jujube in MS + 2.0 mg/L 6-BA + 1.2mg /L NAA reached 86.6%. The multiplication coefficient reached about 5. The optimal rooting medium was 1/2MS + 1.5 mg/L NAA, the rooting rate was 93.75%, and each plant had 4~10 roots, thick and strong roots. The regenerated plants with root length of 3.0~5.0 cm were transplanted into the mixture of peat: garden soil: perlite = 2:2:1, and the survival rate was over 86%.