乌司他丁治疗水下爆炸致兔急性肺损伤对细胞因子表达的影响

Investigation to the Expression of Cytokines in Serum of Rabbits with Acute Lung Injury Interposed by Different Doses Ulinastatin in Underwater Explosion

摘要: 目的：探讨血清肿瘤坏死因子-α及中性粒细胞弹性蛋白酶等细胞因子在乌司他丁治疗水下爆炸致兔急性肺损伤时的表达时相变化。方法：将用于实验的动物随机分为对照组、损伤非治疗组、乌司他丁(高、低剂量)治疗组。复制急性肺损伤动物模型，在发生爆炸后立即进行治疗。在治疗4 h，12 h，24 h时取血检测相关细胞因子的浓度。结果：低剂量乌司他丁治疗组在爆炸后12 h (400.6 ± 79.0 ng/L)、24 h (356.4 ± 181.2 ng/L)血清肿瘤坏死因子-α含量相比损伤非治疗组(573.8 ± 178.2 ng/L; 552.3 ± 169.6 ng/L) 明显降低(P = 0.018, P = 0.013)；血清中性粒细胞弹性蛋白酶含量在爆炸后24 h (62.6 ± 19.5 ng/mL)相比损伤非治疗组(97.6 ± 36.2 ng/mL)明显降低(P = 0.007)。高剂量乌司他丁治疗组血清肿瘤坏死因子-α在爆炸后12 h (356.1 ± 131.0 ng/L)较爆炸损伤组(573.8 ± 178.2 ng/L)明显降低(P = 0.004)，血清中性粒细胞弹性蛋白酶含量在爆炸后24 h (72.3 ± 21.3 ng/mL)明显降低(P = 0.036)。结论：高剂量乌司他丁治疗能够下调血清肿瘤坏死因子-α及中性粒细胞弹性蛋白酶的表达水平，有助于急性肺损伤的治疗。

Abstract: Objective: The concentrations of TNF-α and NE were detected for elucidating the change concentra-tion in sera of rabbits with acute lung injury interposed by different doses Ulinastatin therapy in underwater explosion. Methods: Underwater explosion appliance was applied to cause acute lung injury of rabbits. The experimental rabbits were classified into control group, injury group, Ulinas-tatin therapy group (low dose group and high dose therapy group), respectively. Ulinastatin was given to therapy group after the blast instantly. The concentrations of TNF-α and NE in sera were detected at 4, 12 and 24 hours after bursting. Results: Serum TNF-α content in low dose Ulinastatin group was lower at 12 h (400.6 ± 79.0 ng/L) and 24 h (356.4 ± 181.2 ng/L) after explosion than that in group injury group (573.8 ± 178.2 ng/L, 552.3 ± 169.6 ng/L) (P = 0.018, P = 0.013). And similar-ly, serum NE content was significantly lower at 24 h after explosion (62.6 ± 19.5 ng/mL) than that in injury group (97.6 ± 36.2 ng/mL) (P = 0.007). On the other hand, serum TNF-α in high dose group was significantly decreased (P = 0.004) at 12 h after explosion (356.1 ± 131.0 ng/L) compared with that in injury group (573.8 ± 178.2 ng/L). Serum NE content was also significantly lower at 24 h af-ter explosion (72.3 ± 21.3 ng/mL) than that in injury group (P = 0.036). Conclusion: The level of TNF-α and NE in sera was more efficiently decreased by using different doses Ulinastatin therapy, which may be conducive to elucidating the mechanism of therapy to ALI.