宫颈安康凝胶剂的制备及其药效学初探

Preparation of Cervical Ankang Gel and Preliminary Study on Pharmacodynamics

摘要: 目的：探讨宫颈安康复方凝胶剂对宫颈炎模型大鼠的治疗作用。方法：采用正交试验设计优选宫颈安康复方的提取工艺；并以均匀试验设计对宫颈安康凝胶剂的处方进行优化。建立宫颈炎大鼠模型，试剂盒检测大鼠血清中IL-6、Hs-CRP、超SOD、TNF-α的含量。同时HE染色观察大鼠宫颈病理组织变化。结果：宫颈安康复方的最佳提取工艺为：乙醇浓度50%，提取时间1.5 h，提取次数为3次；宫颈安康凝胶剂的最佳处方为卡波姆5.0%、甘油16.4%、氢氧化钠0.3 g。药效学研究结果显示，与模型组相比，宫颈安康凝胶组IL-6、TNF-α含量降低(P < 0.05)，SOD含量升高(P < 0.05)，Hs-CRP水平显著降低(P < 0.01)。HE结果显示，与模型组相比，宫颈安康凝胶组的大鼠宫颈病变组织的炎性细胞浸润明显减轻。结论：成功制备宫颈安康凝胶剂，其可能通过降低宫颈病变组织中IL-6、TNF-α和Hs-CRP水平，升高SOD水平，从而影响炎症反应相关细胞因子，以及减少宫颈病变组织中炎性渗出物的产生，最终达到治疗宫颈炎症的作用。

Abstract: Objective: To investigate the therapeutic effect of Cervical Ankang compound gel on cervicitis model rats. Methods: The extraction process of Cervical Ankang compound was optimized by orthogonal experimental design, and the prescription of Cervical Ankang compound Gel was optimized by uniform experimental design. A rat model of cervicitis was established, and the serum levels of IL-6, Hs-CRP, ultra-SOD and TNF-α were detected by kits. At the same time, HE staining was used to observe the pathological changes of rat cervix. Results: The optimal extraction process of Cervical Ankang compound was as follows, ethanol concentration 50%, extraction time 1.5 h, and extraction times 3 times. The optimal prescription of Cervical Ankang Gel was carbomer 5.0%, glycerol 16.4%, and sodium hydroxide 0.3 g. The results of the pharmacodynamics study showed that compared with the model group, the contents of IL-6 and TNF-α in the Cervical Ankang gel group decreased (P < 0.05), the content of SOD increased (P < 0.05), and the level of Hs-CRP was significantly decreased (P < 0.01). The HE results showed that compared with the model group, the inflammatory cell infiltration in the cervical lesions of the rats in the Cervical Ankang gel group was significantly reduced. Conclusions: The successful preparation of the Cervical Ankang gel may be able to treat cervical inflammation by reducing the levels of IL-6, TNF-α and Hs-CRP in cervical lesions, increasing the levels of SOD, thereby affecting cytokines related to inflammatory response, and reducing the production of inflammatory exudates in cervical lesions.