大蜡螟生物降解聚乙烯塑料研究进展及展望
Research Progress and Prospect on the Biodegradation of Polyethylene Plastic by Galleria mellonella
DOI: 10.12677/HJAS.2022.125050, PDF,    科研立项经费支持
作者: 乔小燕：新疆农业大学资源与环境学院，新疆 乌鲁木齐;新疆农业科学院土壤肥料与农业节水研究所，新疆 乌鲁木齐；王 斌*, 孙九胜, 孙 晨, 崔 磊, 槐国龙：新疆农业科学院土壤肥料与农业节水研究所，新疆 乌鲁木齐；陈波浪：新疆农业大学资源与环境学院，新疆 乌鲁木齐
关键词: 大蜡螟；生物降解；聚乙烯塑料；白色污染；Galleria mellonella； Biodegradation； Polyethylene Plastic； White Pollution
摘要: 聚乙烯塑料在我们的生活生产中具有至关重要的作用，如农用地膜及包装材料等，然而由于聚乙烯在自然条件下极难降解，废弃的聚乙烯塑料给环境带来严重的“白色污染”。废弃聚乙烯塑料污染治理一直是学者们普遍关注的问题，常用的填埋、焚烧等处理措施又给环境造成二次污染，回收利用处理成本较高，生物降解措施是“白色污染”治理的有效途径。近些年一些研究发现，大蜡螟体内有能够降解聚乙烯的微生物菌株，这为废弃聚乙烯塑料生物降解找到一个新的突破口。本文综述了大蜡螟生物降解聚乙烯塑料的研究进展，旨在为实现大蜡螟生物降解废弃聚乙烯塑料提供理论及实践参考依据。
Abstract: The polyethylene plastic plays a vital role in our life and production, such as agricultural plastic film and packaging materials. However, because polyethylene is very difficult to degrade under natural conditions, waste polyethylene plastic brings serious “white pollution” to the environment. The pollution control of waste polyethylene plastic has been a common concern of scholars. Common treatment measures such as landfill and incineration cause secondary pollution to the environment, and the cost of recycling is high. Biodegradation is an effective way to treat “white pollution”. In recent years, some studies have found that there are microbial strains capable of degrading polyethylene in the body of Galleria mellonella, which finds a new breakthrough for the biodegradation of polyethylene plastic. This paper reviews the research progress on the biodegradation of polyethylene plastic by Galleria mellonella, aiming to provide theoretical and practical basis for realizing the biodegradation of polyethylene plastic by Galleria mellonella.
文章引用：乔小燕, 王斌, 孙九胜, 孙晨, 崔磊, 槐国龙, 陈波浪. 大蜡螟生物降解聚乙烯塑料研究进展及展望[J]. 农业科学, 2022, 12(5): 333-339. https://doi.org/10.12677/HJAS.2022.125050

参考文献

[1] 胡灿, 王旭峰, 陈学庚, 汤修映, 赵岩, 严昌荣. 新疆农田残膜污染现状及防控策略[J]. 农业工程学报, 2019, 35(24): 223-234.
[2] 王海基, 王敏, 王吉亮, 卢勇涛, 营雨琨, 何玉泽. 新疆农田残膜污染治理技术与防控策略[J]. 中国农机化学报, 2022, 43(4): 166-174.
[3] 马蕾, 吕金良. 我国农用地膜使用现状及回收机制研究[J]. 农业科技通讯, 2019(11): 19-23.
[4] 热米兰•乌斯曼. 农业地膜污染现状及防治途径[J]. 农家参谋, 2021(19): 25-26.
[5] 雍婕, 程益, 周海燕. 地膜降解途径及机理研究进展[J]. 应用生态学报, 2021, 32(2): 729-736.
[6] 张雨蒙, 申丽霞. 农田土壤残膜的污染现状及应对措施[J]. 天津农业科学, 2018, 24(2): 86-90.
[7] 马彦, 杨虎德. 甘肃省农田地膜污染及防控措施调查[J]. 生态与农村环境学报, 2015, 31(4): 478-483.
[8] Romic, D., Romic, M., Borosic, J. and Poljak, M. (2003) Mulching Decreases Nitrate Leaching in Bell Pepper (Capsicum annuum L.) Cultiva-tion. Agricultural Water Management, 60, 87-97. [Google Scholar] [CrossRef] 
[9] 邹小阳, 牛文全, 刘晶晶, 李元, 张明智, 段晓辉. 残膜对土壤和作物的潜在风险研究进展[J]. 灌溉排水学报, 2017, 36(7): 47-54.
[10] 王亮, 林涛, 严昌荣, 何文清, 王静, 汤秋香. 不同残膜量和灌溉定额对棉花养分和水分利用的影响[J]. 植物营养与肥料学报, 2018, 24(1): 122-133.
[11] 李蔓. 聚乙烯塑料生产和废聚乙烯塑料资源化技术生命周期评价[D]: [硕士学位论文]. 哈尔滨: 哈尔滨工业大学, 2008.
[12] Yoshida, S., Hiraga, K., Takehana, T., Taniguchi, I., Yamaji, H., Maeda, Y., Toyohara, K., Miyamoto, K., Kimura, Y. and Oda, K. (2016) Response to Comment on “A Bacterium That Degrades and Assimilates Poly (Ethylene Terephthalate)”. Science, 353, 759. [Google Scholar] [CrossRef] [PubMed]
[13] Yang, Y., Yang, J., Wu, W.M., Zhao, J., Song, Y.L., Gao, L.C., Yang, R.F. and Jiang, L. (2015) Biodegradation and Mineralization of Poly-styrene by Plastic-Eating Mealworms: Part 2. Role of Gut Microorganisms. Environmental Science and Technology, 49, 12087-12093. [Google Scholar] [CrossRef] [PubMed]
[14] Bombelli, P., Bertocchini, F. and Howe, C.J. (2017) Polyethylene Biodegradation by Caterpillars of the Wax Moth Galleria mellonella. Current Biology, 27, 292-293. [Google Scholar] [CrossRef] [PubMed]
[15] 刘淑琴. 大蜡螟人工饲料优化及资源利用的研究[D]: [硕士学位论文]. 保定: 河北农业大学, 2020.
[16] Ekaterina, P. 大蜡螟降解塑料及其肠道微生物组研究[D]: [硕士学位论文]. 哈尔滨: 哈尔滨工业大学, 2019.
[17] 刘亚飞. 大蜡螟幼虫肠道中聚乙烯降解菌株的筛选、鉴定及其降解特性研究[D]: [硕士学位论文]. 金华: 浙江师范大学, 2019.
[18] Zhang, J., Gao, D., Li, Q., Zhao, Y.X., Li, L., Lin, H.F., Bi, Q.R. and Zhao, Y.C. (2020) Biodegradation of Polyethylene Microplastic Particles by the Fungus Aspergillus flavus from the Guts of Wax Moth Galleria mellonella. Science of the Total Environment, 704, Article ID: 135931. [Google Scholar] [CrossRef] [PubMed]
[19] Agnès, R., Jehan, W., Ariane, D.B., Nicolas, C., Christina, N.L. and Christophe, S. (2021) Plastic Biodegradation: Do Galleria mellonella Larvae Bioassimilate Polyethylene? A Spectral Histology Approach Using Isotopic Labeling and Infrared Microspectroscopy. Environmental Science and Technology, 56, 525-534. [Google Scholar] [CrossRef] [PubMed]
[20] 毛敏敏. 大蜡螟幼虫肠道中降解聚乙烯微生物的鉴定及表征[D]: [硕士学位论文]. 金华: 浙江师范大学, 2021.
[21] 刘奇志, 田里, 蒲恒浒. 大蜡螟作为试验昆虫资源的利用现状[J]. 昆虫知识, 2009, 46(3): 485-489.
[22] 黄少康, 王建鼎. 大蜡螟的生物学研究[J]. 中国养蜂, 2001, 52(5): 8-10.
[23] Ellis, J.D., Graham, J.R. and Mortensen, A. (2013) Standard Methods for Wax Moth Research. Journal of Apicultural Research, 52, 1-17. [Google Scholar] [CrossRef] 
[24] Wang, H.X., Yuan, X., Huang, H.M., Zhang, B., Cao, C.N. and Zhao, H.P. (2017) Chemical Constituents from Swertia mussotii Franch (Gentianaceae). Nat-ural Product Research, 31, 1704-1708. [Google Scholar] [CrossRef] [PubMed]
[25] Alghoribi, M.F., Gibreel, T.M., Dodgson, A.R., Beatson, S.A. and Upton, M. (2014) Galleria mellonella Infection Model Demonstrates High Lethality of ST69 and ST127 Uro-pathogenic E. coli. PLoS ONE, 9, Article ID: e101547. [Google Scholar] [CrossRef] [PubMed]
[26] Harding, C.R., Schroeder, G.N., Reynolds, S., Kosta, A., Col-lins, J.W., Mousnier, A. and Frankel, G. (2012) Legionella pneumophila Pathogenesis in the Galleria mellonella Infec-tion Model. Infection Immunity, 80, 2780-2790. [Google Scholar] [CrossRef] 
[27] Mahar, A.N., Munir, M., Elawad, S., Gowen, S.R. and Hague, N.G.M. (2005) Pathogenicity of Bacterium, Xenorhabdus Nematophila Isolated from Entomopathogenic Nematode (Steinernema carpocapsae) and Its Secretion against Galleria mellonella Larvae. Journal of Zhejiang University Science, 6, 457-463.
[28] 胡福良, 李英华. 大蜡螟的生物学特性和防治方法[J]. 养蜂科技, 2000(1): 4-6.
[29] 刘瑞, 刘奇志. 国内外大蜡螟研究与产业发展现状及展望[J]. 中国农学通报, 2015, 31(28): 280-284.
[30] 薛琪琪, 门丽娜, 张倩, 祁靖宇, 柳春雨, 冯丹丹, 张宇宏, 张志伟. 饲养密度对大蜡螟实验种群生命表参数的影响[J]. 中国农业科技导报, 2020, 22(7): 69-78.
[31] Kwadha, C.A., Ong’amo, G.O., Ndegwa, P.N., Raina, S.K., Fombong, A.T., Stout, M.J., Davis, J., Diaz, R., Beuzelin, J.M. (2017) The Biology and Control of the Greater Wax Moth Galleria mellonella. Insects, 8, Article No. 61. [Google Scholar] [CrossRef] [PubMed]
[32] 杨爽, 张学文, 宋文菲, 苗春辉, 赵慧婷, 姜玉锁. 大蜡螟生物学特性及其防治研究概述[J]. 中国蜂业, 2016, 67(3): 33-37.
[33] 赵军. 大蜡螟的室内人工饲养技术及生物学观察[J]. 安徽农学通报, 2010, 16(10): 38-40+119.
[34] 刘位芬, 梁铖, 宋文菲, 苗春辉, 张学文, 杨爽. 一种易于实验室批量繁殖大蜡螟的方法[J]. 中国蜂业, 2016, 67(6): 34-35.
[35] 熊延坤, 张青文, 徐静, 段灿星, 周明牂. 大蜡螟工厂化生产流程及环境因素的控制[J]. 中国农业大学学报, 2002, 7(5): 85-89.
[36] Jyothi, J.V.A. (2006) Effect of Lighting Conditions and Substratum on Egg Laying Behaviour of Galleria mellonella (L). Zoological Society of India, 5, 97-l05.
[37] 蒲恒浒, 刘奇志. 不同饲料配方对大蜡螟生长影响评价[J]. 安徽农业科学, 2009, 37(28): 13647-13648+13668.
[38] 吴艳艳, 周婷, 王强, 代平礼, 龚星鑫, 罗其花等. 大蜡螟饲料配方的优化[J]. 昆虫知识, 2010, 47(2): 409-413.
[39] 黄诚华, 潘雪红, 黄冬发, 王伯辉, 魏吉利. 大蜡螟人工饲料配方筛选试验[J]. 广西农业科学, 2010, 41(7): 672-674.
[40] 张俊俊, 王桂珍, 尹志龙, 刘平臻, 汪正威. 大蜡螟卵粒快速计数法[J]. 养蜂技术, 2020, 71(7): 24-26.
[41] 杨爽, 刘位芬, 梁铖, 张祖芸, 苗春辉, 徐凯, 等. 蜂群中大蜡螟蛹及成虫雌雄的鉴别方法[J]. 蜜蜂杂志, 2017, 37(3): 8-10.
[42] Yang, J., Yang, Y., Wu, W.M., Zhao, J. and Jiang, L. (2014) Evidence of Polyethylene Biodegradation by Bacterial Strains from the Guts of Plastic-Eating Wax Worms. Environmen-tal Science and Technology, 48, 13776-13784. [Google Scholar] [CrossRef] [PubMed]
[43] 胡亚楠, 贺旭, 亚森•沙力, 罗明, 张宇宏, 张帅. 大蜡螟和黄粉虫肠道菌中聚乙烯地膜降解细菌的筛选及其降解性能[J]. 微生物学通报, 2020, 47(12): 4029-4041.
[44] Saikia, S.S., Borah, B.K., Baruah, G., Rokozeno and Deka, M.K. (2022) Characterization of the Gut Microbes of Greater Wax Moth (Galleria mellonella L.) Shows Presence of Potential Polymer Degraders. Folia Microbiologica, 67, 133-141. [Google Scholar] [CrossRef] [PubMed]
[45] Asal, P., Hedayat, B., Leonid, G., Nadieh, D.J. and Lund, N.J. (2021) Mastication of Polyolefins Alters the Microbial Composition in Galleria mellonella. Environmental Pollution, 280, Article ID: 116877 [Google Scholar] [CrossRef] [PubMed]
[46] 唐瑞, 林佳倚, 劳乔斌, 徐梓锐, 谭梓铭, 吴正伟. 取食聚乙烯微塑料对大蜡螟生长发育的影响[J]. 安徽农学通报, 2020, 26(23): 93-96.
[47] 唐启河, 董志祥, 李还原, 陈奕霏, 郭军. 昆虫降解塑料的机制及其研究方法[J]. 生物学杂志, 2021, 38(5): 96-100.

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