E-CAD及PLAC8在不明原因稽留流产蜕膜组织中的表达
Expression of E-CAD and PLAC8 in Decidual Tissue of Unexplained Missed Abortion
摘要: 目的:探讨E-钙黏蛋白和胎盘特异性蛋白8在不明原因稽留流产蜕膜组织中的表达及其可能的意义。方法:选取2020年10月至2021年1月至青岛大学附属妇女儿童医院妇科就诊的早孕稽留流产患者20例,非计划妊娠行人工流产的正常早孕女性20例作为研究对象。采用实时荧光定量聚合酶链反应(qRT-PCR),Western Blot的方法检测两组蜕膜组织中E-钙黏蛋白和PLAC8的表达水平。结果:不明原因稽留流产组蜕膜组织中E-钙黏蛋白的mRNA及蛋白表达明显低于正常对照组,PLAC8的mRNA及蛋白表达明显高于正常对照组。结论:E-钙黏蛋白的低表达及PLAC8的过表达可能与不明原因稽留流产的发生有关。我们的实验结果可能有助于揭示不明原因稽留流产新的研究方向。
Abstract: Objective: To investigate the expression and possible significance of E-cadherin and Placental spe-cific protein 8 (PLAC8) in decidual tissue of unexplained missed abortion. Methods: A total of 20 pa-tients with missed abortion in early pregnancy and 20 normal early pregnant women were selected from October 2020 to January 2021 in Women and Children’s Hospital affiliated to Qingdao Univer-sity. Quantitative Real-time chain reaction (qRT-PCR) and Western Blot were used to detect the ex-pression of E-cadherin and PLAC8 in the decidual of the two groups. Results: The expression of mRNA and protein of E-cadherin in decidual tissue of unexplained missed abortion group was sig-nificantly lower than that of normal control, while the expression of mRNA and protein of PLAC8 was significantly higher than that of normal control. Conclusion: The low expression of E-cadherin and the overexpression of PLAC8 may be related to the occurrence of unexplained missed abortion. Our experimental results may be helpful to reveal the new research direction of unexplained missed abortion.
文章引用:冷喆, 孙娴莉, 林慧, 常晓彤, 宋黄贝, 黄煜. E-CAD及PLAC8在不明原因稽留流产蜕膜组织中的表达[J]. 临床医学进展, 2022, 12(5): 4096-4104. https://doi.org/10.12677/ACM.2022.125593

参考文献

[1] Marwah, S., Gupta, S., Batra, N., et al. (2016) A Comparative Study to Evaluate the Efficacy of Vaginal vs Oral Prosta-glandin E1 Analogue (Misoprostol) in Management of First Trimester Missed Abortion. Journal of Clinical and Diag-nostic Research: JCDR, 10, QC14-QC18. [Google Scholar] [CrossRef
[2] Linnakaari, R., Helle, N., Mentula, M., et al. (2019) Trends in the Incidence, Rate and Treatment of Miscarriage-Na- tionwide Regis-ter-Study in Finland, 1998-2016. Human Reproduction, 34, 2120-2128. [Google Scholar] [CrossRef] [PubMed]
[3] Clifford, K., Rai, R., Watson, H., et al. (1994) An Informative Protocol for the Investigation of Recurrent Miscarriage: Preliminary Experience of 500 Consecutive Cases. Human Reproduction (Oxford, England), 9, 1328-1332. [Google Scholar] [CrossRef] [PubMed]
[4] Hatasaka, H. (1994) Recurrent Miscarriage: Epidemi-ologic Factors, Definitions, and Incidence. Clinical Obstetrics and gynecology, 37, 625-634. [Google Scholar] [CrossRef] [PubMed]
[5] Bulletti, C., Flamigni, C. and Giacomucci, E. (1996) Re-productive Failure Due to Spontaneous Abortion and Recurrent Miscarriage. Human Reproduction Update, 2, 118-136. [Google Scholar] [CrossRef] [PubMed]
[6] Braga, V. (2000) Epithelial Cell Shape: Cadherins and Small GTPases. Experimental Cell Research, 261, 83-90. [Google Scholar] [CrossRef] [PubMed]
[7] Takeichi, M. (1977) Functional Correlation between Cell Adhesive Properties and Some Cell Surface Proteins. The Journal of Cell Biology, 75, 464-474. [Google Scholar] [CrossRef] [PubMed]
[8] Wong, S., Fang, C., Chuah, L., et al. (2018) E-Cadherin: Its Dysregula-tion in Carcinogenesis and Clinical Implications. Critical Reviews in Oncology/Hematology, 121, 11-22. [Google Scholar] [CrossRef] [PubMed]
[9] Jiang, W. (1996) E-Cadherin and Its Associated Protein Catenins, Cancer Invasion and Metastasis. The British Journal of Surgery, 83, 437-446. [Google Scholar] [CrossRef] [PubMed]
[10] Capaldo, C., Farkas, A. and Nusrat, A. (2014) Epithelial Adhesive Junctions. F1000Prime Reports, 6, 1. [Google Scholar] [CrossRef
[11] Galaviz-Hernandez, C., Stagg, C., de Ridder, G., et al. (2003) Plac8 and Plac9, Novel Placental-Enriched Genes Identified through Microarray Analysis. Gene, 309, 81-89. [Google Scholar] [CrossRef
[12] Rissoan, M., Duhen, T., Bridon, J., et al. (2002) Subtractive Hybridization Reveals the Expression of Immunoglobulin-Like Transcript 7, Eph-B1, Granzyme B, and 3 Novel Tran-scripts in Human Plasmacytoid Dendritic Cells. Blood, 100, 3295-303. [Google Scholar] [CrossRef] [PubMed]
[13] Rogulski, K., Li, Y., Rothermund, K., et al. (2005) Onzin, a c-Myc-Repressed Target, Promotes Survival and Transformation by Modulating the Akt-Mdm2-p53 Pathway. Onco-gene, 24, 7524-7541. [Google Scholar] [CrossRef] [PubMed]
[14] Ledford, J., Kovarova, M. and Koller, B. (2007) Impaired Host De-fense in Mice Lacking ONZIN. Journal of Immunology (Baltimore, Md: 1950), 178, 5132-5143. [Google Scholar] [CrossRef] [PubMed]
[15] Jimenez-Preitner, M., Berney, X., Uldry, M., et al. (2011) Plac8 Is an Inducer of C/EBPβ Required for Brown Fat Differentiation, Thermoregulation, and Control of Body Weight. Cell Metabolism, 14, 658-670. [Google Scholar] [CrossRef] [PubMed]
[16] Jimenez-Preitner, M., Berney, X. and Thorens, B. (2012) Plac8 Is Required for White Adipocyte Differentiation in Vitro and Cell Number Control in Vivo. PLoS ONE, 7, e48767. [Google Scholar] [CrossRef] [PubMed]
[17] Johnson, R., Kerr, M. and Slaven, J. (2012) Plac8-Dependent and Inducible NO Synthase-Dependent Mechanisms Clear Chlamydia muridarum Infections from the Genital Tract. Journal of Immunology (Baltimore, Md: 1950), 188, 1896-1904. [Google Scholar] [CrossRef] [PubMed]
[18] Zinaman, M., Clegg, E., Brown, C., et al. (1996) Estimates of Hu-man Fertility and Pregnancy Loss. Fertility and Sterility, 65, 503-509. [Google Scholar] [CrossRef
[19] Hülsken, J., Birchmeier, W. and Behrens, J. (1994) E-Cadherin and APC Compete for the Interaction with Beta-Catenin and the Cytoskeleton. The Journal of Cell Biology, 127, 2061-2069. [Google Scholar] [CrossRef] [PubMed]
[20] Jeanes, A., Gottardi, C. and Yap, A. (2008) Cadher-ins and Cancer: How Does Cadherin Dysfunction Promote Tumor Progression? Oncogene, 27, 6920-6929. [Google Scholar] [CrossRef] [PubMed]
[21] Schmalhofer, O., Brabletz, S. and Brabletz, T. (2009) E-cadherin, Be-ta-Catenin, and ZEB1 in Malignant Progression of Cancer. Cancer Metastasis Reviews, 28, 151-166. [Google Scholar] [CrossRef] [PubMed]
[22] Riethmacher, D., Brinkmann, V. and Birchmeier, C. (1995) A Targeted Mutation in the Mouse E-Cadherin Gene Results in Defective Preimplantation Development. Proceedings of the National Academy of Sciences of the United States of America, 92, 855-859. [Google Scholar] [CrossRef] [PubMed]
[23] Fleming, T., Javed, Q. and Hay, M. (1992) Epithelial Differentiation and Intercellular Junction Formation in the Mouse Early Embryo. Development (Cambridge, England) Supplement, 105-112. [Google Scholar] [CrossRef
[24] Fierro-González, J., White, M., Silva, J., et al. (2013) Cad-herin-Dependent Filopodia Control Preimplantation Embryo Compaction. Nature Cell Biology, 15, 1424-1433. [Google Scholar] [CrossRef] [PubMed]
[25] Kim, K., Lu, Z. and Hay, E. (2002) Direct Evidence for a Role of Be-ta-Catenin/LEF-1 Signaling Pathway in Induction of EMT. Cell Biology International, 26, 463-476. [Google Scholar] [CrossRef] [PubMed]
[26] Lee, J., Dedhar, S., Kalluri, R., et al. (2006) The Epitheli-al-Mesenchymal Transition: New Insights in Signaling, Development, and Disease. The Journal of Cell Biology, 172, 973-981. [Google Scholar] [CrossRef] [PubMed]
[27] Uehara, H., Takahashi, T. and Izumi, K. (2013) Induction of Retinol-Binding Protein 4 and Placenta-Specific 8 Expression in Human Prostate Cancer Cells Remaining in Bone Fol-lowing Osteolytic Tumor Growth Inhibition by Osteoprotegerin. International Journal of Oncology, 43, 365-374. [Google Scholar] [CrossRef] [PubMed]
[28] Kinsey, C., Balakrishnan, V., O’Dell, M., et al. (2014) Plac8 Links Oncogenic Mutations to Regulation of Autophagy and Is Critical to Pancreatic Cancer Progression. Cell Reports, 7, 1143-1155. [Google Scholar] [CrossRef] [PubMed]
[29] Kolluru, V., Pal, D., Papu John, A.M.S., et al. (2017) Induction of Plac8 Promotes Pro-Survival Function of Autophagy in Cadmium-Induced Prostate Carcinogenesis. Cancer Letter, 408, 121-129. [Google Scholar] [CrossRef] [PubMed]
[30] Zou, L., Chai, J., Gao, Y., et al. (2016) Down-Regulated PLAC8 Promotes Hepatocellular Carcinoma Cell Proliferation by Enhancing PI3K/Akt/GSK3β/Wnt/β-Catenin Signaling. Bio-medicine & Pharmacotherapy, 84, 139-146. [Google Scholar] [CrossRef] [PubMed]
[31] Li, C., Ma, H., Wang, Y., et al. (2014) Excess PLAC8 Promotes an Unconventional ERK2-Dependent EMT in Colon Cancer. The Journal of Clinical Investigation, 124, 2172-2187. [Google Scholar] [CrossRef
[32] Mao, M., Chen, Y., Jia, Y., et al. (2019) PLCA8 Suppresses Breast Cancer Apoptosis by Activating the PI3k/AKT/ NF-κB Pathway. Journal of Cellular and Molecular Medicine, 23, 6930-6941. [Google Scholar] [CrossRef] [PubMed]
[33] Huang, M.L., Zou, Y., Yang, R., et al. (2019) Placenta Specific 8 Gene Induces Epithelial-Mesenchymal Transition of Nasopharyngeal Carcinoma Cells via the TGF-β/Smad Pathway. Experi-mental Cell Research, 374, 172-180. [Google Scholar] [CrossRef] [PubMed]
[34] Mao, M., Hu, D., Yang, J., et al. (2021) Regulation of Tamoxifen Sensitivity by the PLAC8/MAPK Pathway Axis Is Antagonized by Curcumin-Induced Protein Stability Change. Journal of Molecular Medicine (Berl), 99, 845-858. [Google Scholar] [CrossRef] [PubMed]
[35] Qin, X.H., Wang, H.X., Ma, L., et al. (2020) Knockout of the Placenta Specific 8 Gene Affects the Proliferation and Migration of Human Embryonic Kidney 293T Cell. Cell Biochem-istry and Biophysics, 78, 55-64. [Google Scholar] [CrossRef] [PubMed]
[36] Huang, M.L., Qi, C.L., Zou, Y., et al. (2020) Plac8-Mediated Autophagy Regulates Nasopharyngeal Carcinoma Cell Function via AKT/mTOR Pathway. Journal of Cellular and Mo-lecular Medicine, 24, 7778-7788. [Google Scholar] [CrossRef] [PubMed]
[37] Mao, M., Cheng, Y., Yang, J., et al. (2021) Multifaced Roles of PLAC8 in Cancer. Biomarker Research, 9, 73. [Google Scholar] [CrossRef] [PubMed]
[38] El-Sayed, A., Hoelker, M., Rings, F., et al. (2006) Large-Scale Transcriptional Analysis of Bovine Embryo Biopsies in Relation to Pregnancy Success after Transfer to Recipients. Physiological Genomics, 28, 84-96. [Google Scholar] [CrossRef] [PubMed]
[39] Gómez, E., Caamaño, J., Bermejo-Alvarez, P., et al. (2009) Gene Expression in Early Expanded Parthenogenetic and in Vitro Fertilized Bovine Blastocysts. The Journal of Reproduction and Development, 55, 607-614. [Google Scholar] [CrossRef
[40] Ghanem, N., Salilew-Wondim, D., Gad, A., et al. (2011) Bovine Blasto-cysts with Developmental Competence to Term Share Similar Expression of Developmentally Important Genes although Derived from Different Culture Environments. Reproduction (Cambridge, England), 142, 551-164. [Google Scholar] [CrossRef
[41] Li, M., Liu, D., Wang, L., et al. (2016) Expression of Placenta-Specific 8 in Human Oocytes, Embryos, and Models of in Vitro Implantation. Fertility and Sterility, 106, 781-789.e2. [Google Scholar] [CrossRef] [PubMed]
[42] El-Sheikh Ali, H., Scoggin, K., Linhares Boakari, Y., et al. (2021) Kinetics of Placenta-Specific 8 (PLAC8) in Equine Placenta during Pregnancy and Placentitis. Theriogenology, 160, 81-89. [Google Scholar] [CrossRef] [PubMed]
[43] Chang, W.L., Liu, Y.W., Dang, Y.L., et al. (2018) PLAC8, a New Marker for Human Interstitial Extravillous Trophoblast Cells, Promotes Their Invasion and Migration. Development, 145, dev148932. [Google Scholar] [CrossRef] [PubMed]
[44] DaSilva-Arnold, S., James, J., Al-Khan, A., et al. (2015) Differentiation of First Trimester Cytotrophoblast to Extravillous Trophoblast Involves an Epithelial-Mesenchymal Transition. Placenta, 36, 1412-1418. [Google Scholar] [CrossRef] [PubMed]
[45] Kam, E., Gardner, L., Loke, Y., et al. (1999) The Role of Trophoblast in the Physiological Change in Decidual Spiral Arteries. Human Reproduction (Oxford, England), 14, 2131-2138. [Google Scholar] [CrossRef] [PubMed]
[46] Pijnenborg, R., Dixon, G., Robertson, W.B., et al. (1980) Trophoblastic Invasion of Human Decidua from 8 to 18 Weeks of Pregnancy. Placenta, 1, 3-19. [Google Scholar] [CrossRef
[47] Zhang, Y., Hu, Q., Li, G., et al. (2018) ONZIN Upregulation by Mutant p53 Contributes to Osteosarcoma Metastasis through the CXCL5-MAPK Signaling Pathway. Cellular Physi-ology and Biochemistry, 48, 1099-1111. [Google Scholar] [CrossRef] [PubMed]
[48] Qi, C., Hong, L., Cheng, Z., et al. (2016) Identification of Metasta-sis-Associated Genes in Colorectal Cancer Using metaDE and Survival Analysis. Oncology Letters, 11, 568-574. [Google Scholar] [CrossRef] [PubMed]