生物素主要检测方法综述
Review on Main Detection Methods of Biotin
DOI: 10.12677/AAC.2022.122014, PDF,  被引量    科研立项经费支持
作者: 齐鑫鹏, 林子谣, 吕秋月, 李 琦, 莫晓凤, 韩国成*：桂林电子科技大学，广西 桂林
关键词: 生物素；检测；生物传感器；电化学；色谱法；Biotin； Detection； Biosensor； Electrochemistry； Chromatography
摘要: 生物素(biotin)别称维生素B7，也可称其为维生素H、辅酶R等，在碳水化合物、氨基酸和脂肪酸的代谢中作为几种羧化酶的必需辅酶发挥重要作用，对于构成视沉细胞内感光物质、维持上皮组织结构的完整和健全、增强机体免疫反应和抵抗力、维持正常生长发育等有重要作用。因此检测各类日常食物中的生物素以及人类每天摄入生物素的含量意义重大。目前检测生物素的方法有微生物法、色谱法、荧光法、分光光度法、免疫法、生物传感器法等，本文主要对于生物素主要检测方法进行综述，尤其是生物传感器方法。
Abstract: Biotin, also known as vitamin B7, can also be called vitamin H and coenzyme R; it plays an important role as an essential coenzyme of several carboxylase enzymes in the metabolism of carbohydrates, amino acids and fatty acids and also in forming photosensitive substances in retinal cells, maintaining the integrity and soundness of epithelial tissue structure, enhancing immune response and resistance, and maintaining normal growth and development. Therefore, it is of great significance to detect the content of biotin in various daily foods and the daily intake of biotin by humans. At present, the detection methods of biotin include microbial method, chromatography, fluorescence method, spectrophotometry, immunoassay, biosensor method and so on. This paper mainly summarizes the main detection methods of biotin, especially biosensor methods.
文章引用：齐鑫鹏, 林子谣, 吕秋月, 李琦, 莫晓凤, 韩国成. 生物素主要检测方法综述[J]. 分析化学进展, 2022, 12(2): 102-110. https://doi.org/10.12677/AAC.2022.122014

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