芬芥素生物合成研究进展
Research Progress of Fengycin Biosynthesis
DOI: 10.12677/AAC.2022.122016, PDF,    科研立项经费支持
作者: 闫更轩, 田 缘*：黑龙江省科学院微生物研究所，黑龙江 哈尔滨；高欣策：乌海职业技术学院医学系，内蒙古 乌海
关键词: 芬芥素；生物合成；芽孢杆菌；发酵工程；Fengycin； Biosynthesis； Bacillus； Fermentation Engineering
摘要: 芬芥素是一类具备优异抗丝状真菌活性的环状脂肽，有望在植物保护、医疗健康、食品防腐等领域发挥重要作用。芬芥素的制备主要依赖于芽孢杆菌的生物合成。介绍了近年来在芬芥素合成途径、芬芥素合成关键基因挖掘、芬芥素合成工程菌的开发等方面取得的新进展，以期为芬芥素的生物合成、生物活性与合成生物学的深入研究提供参考。
Abstract: Fengycin is a kind of cyclic lipopeptides with strong antifungal activity against filamentous fungi, which is expected to play an important role in plant protection, health care, food antisepsis and other fields. The preparation of fengycin mainly depends on the biosynthesis of bacillus species. The recent progress in the synthesis pathway, key gene discovery, and the development of engineering bacteria for fengycin synthesis are reviewed, in order to provide reference for the further study of fengycin biosynthesis, biological activity and synthetic biology.
文章引用：闫更轩, 高欣策, 田缘. 芬芥素生物合成研究进展[J]. 分析化学进展, 2022, 12(2): 125-131. https://doi.org/10.12677/AAC.2022.122016

参考文献

[1] Schneider, J., Taraz, K., Budzikiewicz, H., et al. (1999) The Structure of Two Fengycins from Bacillus subtilis S499. Zeitschrift für Naturforschung C, 54, 859-866.
[Google Scholar] [CrossRef] [PubMed]
[2] Zakharova, A.A., Efimova, S.S., Malev, V.V., et al. (2019) Fengycin Induces Ion Channels in Lipid Bilayers Mimicking Target Fungal Cell Membranes. Scientific Reports, 9, Article No. 16034.
[Google Scholar] [CrossRef] [PubMed]
[3] Hu, L.B., Shi, Z.Q., Zhang, T., et al. (2007) Fengycin Antibiotics Isolated from B-FS01 Culture Inhibit the Growth of Fusarium moniliforme Sheldon ATCC 38932. FEMS Microbiology Letters, 272, 91-98.
[Google Scholar] [CrossRef] [PubMed]
[4] Touré, Y., Ongena, M., Jacques, P., et al. (2004) Role of Lipopeptides Produced by Bacillus subtilis GA1 in the Reduction of Grey Mould Disease Caused by Botrytis cinerea on Apple. Journal of Applied Microbiology, 96, 1151-1160.
[Google Scholar] [CrossRef] [PubMed]
[5] Hanif, A., Zhang, F., Li, P., et al. (2019) Fengycin Produced by Bacillus Amyloliquefaciens FZB42 Inhibits Fusarium graminearum Growth and Mycotoxins Biosynthesis. Toxins, 11, 295.
[6] Meena, K.R. and Kanwar, S.S. (2015) Lipopeptides as the Antifungal and Antibacterial Agents: Applications in Food Safety and Therapeutics. BioMed Research International, 2015, Article ID: 473050.
[Google Scholar] [CrossRef] [PubMed]
[7] Cheng, W., Feng, Y.Q., Ren, J., et al. (2016) Anti-Tumor Role of Bacillus subtilis fmbJ-Derived Fengycin on Human Colon Cancer HT29 Cell Line. Neoplasma, 63, 215-222.
[Google Scholar] [CrossRef] 
[8] Samel, S.A., Wagner, B., Marahiel, M.A., et al. (2006) The Thioesterase Domain of the Fengycin Biosynthesis Cluster: A Structural Base for the Macrocyclization of a Non-Ribosomal Lipopeptide. Journal of Molecular Biology, 359, 876-889.
[Google Scholar] [CrossRef] [PubMed]
[9] Rosés, C., Camó, C., Oliveras, À., et al. (2018) Total Solid-Phase Synthesis of Dehydroxy Fengycin Derivatives. The Journal of Organic Chemistry, 83, 15297-15311.
[Google Scholar] [CrossRef] [PubMed]
[10] Wu, C.Y., Chen, C.L., Lee, Y.H., et al. (2007) Nonribosomal Synthesis of Fengycin on an Enzyme Complex Formed by Fengycin Synthetases. Journal of Biological Chemistry, 282, 5608-5616.
[Google Scholar] [CrossRef] 
[11] Stein, T. (2005) Bacillus subtilis Antibiotics: Structures, Syntheses and Specific Functions. Molecular Microbiology, 56, 845-857.
[Google Scholar] [CrossRef] [PubMed]
[12] Sieber, S.A., Walsh, C.T. and Marahiel, M.A. (2003) Loading Peptidyl-Coenzyme A onto Peptidyl Carrier Proteins: A Novel Approach in Characterizing Macrocyclization by Thioesterase Domains. Journal of the American Chemical Society, 125, 10862-10866.
[Google Scholar] [CrossRef] [PubMed]
[13] Gao, L., Guo, J., Fan, Y., et al. (2018) Module and Individual Domain Deletions of NRPS to Produce Plipastatin Derivatives in Bacillus subtilis. Microbial Cell Factories, 17, Article No. 84.
[Google Scholar] [CrossRef] [PubMed]
[14] Zeng, Q., Xie, J., Li, Y., et al. (2021) Organization, Evolution and Function of Fengycin Biosynthesis Gene Clusters in the Bacillus amyloliquefaciens Group. Phytopathology Research, 3, 1-12.
[15] Yang, R., Lei, S., Xu, X., et al. (2020) Key Elements and Regulation Strategies of NRPSs for Biosynthesis of Lipopeptides by Bacillus. Applied Microbiology and Biotechnology, 104, 8077-8087.
[Google Scholar] [CrossRef] [PubMed]
[16] Ke, W.J., Chang, B.Y., Lin, T.P., et al. (2009) Activation of the Promoter of the Fengycin Synthetase Operon by the UP Element. Journal of Bacteriology, 191, 4615-4623.
[Google Scholar] [CrossRef] 
[17] Romero, D., de Vicente, A., Rakotoaly, R.H., et al. (2007) The Iturin and Fengycin Families of Lipopeptides Are Key Factors in Antagonism of Bacillus subtilis toward Podosphaera fusca. Molecular Plant-Microbe Interactions, 20, 430-440.
[Google Scholar] [CrossRef] 
[18] Sun, J., Liu, Y., Lin, F., et al. (2021) CodY, ComA, DegU and Spo0A Controlling Lipopeptides Biosynthesis in Bacillus amyloliquefaciens fmbJ. Journal of Applied Microbiology, 131, 1289-1304.
[Google Scholar] [CrossRef] [PubMed]
[19] Wang, P., Guo, Q., Ma, Y., et al. (2015) DegQ Regulates the Production of Fengycins and Biofilm Formation of the Biocontrol Agent Bacillus subtilis NCD-2. Microbiological Research, 178, 42-50.
[Google Scholar] [CrossRef] [PubMed]
[20] Guo, Q., Dong, L., Wang, P., et al. (2018) The PhoR/PhoP Two-Component System Regulates Fengycin Production in Bacillus subtilis NCD-2 under Low-Phosphate Conditions. Journal of Integrative Agriculture, 17, 149-157.
[Google Scholar] [CrossRef] 
[21] Yaseen, Y., Diop, A., Gancel, F., et al. (2018) Polynucleotide Phosphorylase Is Involved in the Control of Lipopeptide Fengycin Production in Bacillus subtilis. Archives of Microbiology, 200, 783-791.
[Google Scholar] [CrossRef] [PubMed]
[22] Cameron, T.A., Matz, L.M. and De Lay, N.R. (2018) Polynucleotide Phosphorylase: Not Merely an RNase but a Pivotal Post-Transcriptional Regulator. PLoS Genetics, 14, e1007654.
[Google Scholar] [CrossRef] [PubMed]
[23] Vahidinasab, M., Lilge, L., Reinfurt, A., et al. (2020) Construction and Description of a Constitutive Plipastatin Mono-Producing Bacillus subtilis. Microbial Cell Factories, 19, 1-12.
[Google Scholar] [CrossRef] [PubMed]
[24] Coutte, F., Lecouturier, D., Ait Yahia, S., et al. (2010) Production of Surfactin and Fengycin by Bacillus subtilis in a Bubbleless Membrane Bioreactor. Applied Microbiology and Biotechnology, 87, 499-507.
[Google Scholar] [CrossRef] [PubMed]
[25] Rangarajan, V., Dhanarajan, G. and Sen, R. (2015) Bioprocess Design for Selective Enhancement of Fengycin Production by a Marine Isolate Bacillus megaterium. Biochemical Engineering Journal, 99, 147-155.
[Google Scholar] [CrossRef] 
[26] Fahim, S., Dimitrov, K., Gancel, F., et al. (2012) Impact of Energy Supply and Oxygen Transfer on Selective Lipopeptide Production by Bacillus subtilis BBG21. Bioresource Technology, 126, 1-6.
[Google Scholar] [CrossRef] [PubMed]
[27] Hmidet, N., Ben Ayed, H., Jacques, P., et al. (2017) Enhancement of Surfactin and Fengycin Production by Bacillus mojavensis A21: Application for Diesel Biodegradation. BioMed Research International, 2017, Article ID: 5893123.
[Google Scholar] [CrossRef] [PubMed]
[28] Chtioui, O., Dimitrov, K., Gancel, F., et al. (2014) Selective Fengycin Production in a Modified Rotating Discs Bioreactor. Bioprocess and Biosystems Engineering, 37, 107-114.
[Google Scholar] [CrossRef] [PubMed]
[29] Yaseen, Y., Gancel, F., Béchet, M., et al. (2017) Study of the Correlation between Fengycin Promoter Expression and Its Production by Bacillus subtilis under Different Culture Conditions and the Impact on Surfactin Production. Archives of Microbiology, 199, 1371-1382.
[Google Scholar] [CrossRef] [PubMed]
[30] Lu, H., Qian, S., Muhammad, U., et al. (2016) Effect of Fructose on Promoting Fengycin Biosynthesis in Bacillus amyloliquefaciens fmb-60. Journal of Applied Microbiology, 121, 1653-1664.
[Google Scholar] [CrossRef] [PubMed]
[31] Medeot, D.B., Bertorello-Cuenca, M., Liaudat, J.P., et al. (2017) Improvement of Biomass and Cyclic Lipopeptides Production in Bacillus amyloliquefaciens MEP218 by Modifying Carbon and Nitrogen Sources and Ratios of the Culture Media. Biological Control, 115, 119-128.
[Google Scholar] [CrossRef] 
[32] 陈燕萍, 陈梅春, 蓝江林, 郑梅霞, 刘波, 王阶平. 培养基配方对贝莱斯芽胞杆菌FJAT-45028脂肽产量和抑菌活性的影响[J]. 福建农业科技, 2021, 52(12): 26-31.
[33] 李响, 伍辉军, 张阳, 鲁晴辉, 高学文. degQ和sfp提高芽孢杆菌泛革素产量及其机理研究[J]. 中国生物防治学报, 2015, 31(2): 221-228.
[34] He, M., Wen, J., Yin, Y., et al. (2021) Metabolic Engineering of Bacillus subtilis Based on Genome-Scale Metabolic Model to Promote Fengycin Production. 3 Biotech, 11, Article No. 448.
[Google Scholar] [CrossRef] [PubMed]
[35] 周振. 解淀粉芽孢杆菌Fengycin合成酶基因表达调控的研究[D]: [硕士学位论文]. 淮安: 淮阴工学院, 2020.
[36] Zhao, J., Zhang, C., Lu, J., et al. (2016) Enhancement of Fengycin Production in Bacillus amyloliquefaciens by Genome Shuffling and Relative Gene Expression Analysis Using RT-PCR. Canadian Journal of Microbiology, 62, 431-436.
[Google Scholar] [CrossRef] [PubMed]

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