COPD患者诱导痰中IL-6、IL-8水平与炎症表型的相关性研究
Study on the Correlation between IL-6 and IL-8 Levels in Induced Sputum of COPD Patients and Inflammatory Phenotypes
DOI: 10.12677/ACM.2022.125621, PDF,   
作者: 史秀枝：成都市第七人民医院呼吸与危重症医学科，四川 成都 ；曹晓红*：山西医科大学附属汾阳医院老年医学科，山西 汾阳
关键词: 慢性阻塞性肺疾病；诱导痰；白介素-6；白介素-8；嗜酸性粒细胞表型；Chronic Obstructive Pulmonary Disease； Induced Sputum； IL-6； IL-8； Eosinophil Phenotype
摘要: 目的：探讨慢性阻塞性肺疾病(慢阻肺，Chronic obstructive pulmonary disease，COPD)患者诱导痰中白介素-6 (interleukin-6, IL-6)、白介素-8 (interleukin-8, IL-8)与炎症表型的相关性，为COPD患者精准治疗提供理论依据及监测指标。方法：选取2019年12月至2020年12月山西医科大学附属汾阳医院30例慢性阻塞性肺疾病急性加重期患者(Acute exacerbation of chronic obstructive pulmonary disease, AECOPD)和30例慢性阻塞性肺疾病稳定期患者(Stable stage of chronic obstructive pulmo-nary disease, SCOPD)，所选患者均行肺功能、诱导痰检查，分析诱导痰中IL-6、IL-8水平与COPD患者GOLD分级(又称肺功能分级)及炎症表型的关系。结果：AECOPD患者诱导痰中IL-6、IL-8水平明显高于SCOPD患者(P < 0.05)，与GOLD分级呈正相关(P < 0.05)，在不同炎症表型中有差异(P < 0.05)，非嗜酸性粒细胞表型 > 嗜酸性粒细胞表型。结论：COPD患者诱导痰中IL-6、IL-8水平与慢阻肺患者急性加重相关，与GOLD分级呈正相关，在嗜酸性粒细胞表型中较非嗜酸性粒细胞表型中表达低。
Abstract: Objective: To explore the correlation between induced sputum interleukin-6 (IL-6) and interleu-kin-8 (IL-8) and inflammatory phenotypes in patients with chronic obstructive pulmonary disease (COPD), so as to provide theoretical basis and monitoring indicators for precision therapy of COPD patients. Methods: To choose between December 2019 and December 2020 in Fenyang Hospital Af-filiated to Shanxi Medical University 30 cases of acute exacerbation of chronic obstructive pulmo-nary disease (AECOPD) and 30 cases of patients with stable stage of chronic obstructive pulmonary disease (SCOPD), lung function and induced sputum examinations were performed in all selected patients to analyze the relationship between IL-6 and IL-8 levels of induced sputum and GOLD grading (pulmonary function classification) and inflammatory phenotypes in COPD patients. Results: The IL-6 and IL-8 levels in the induced sputum of patients with AECOPD were significantly higher than those of patients with SCOPD (P < 0.05), which were positive correlation with the GOLD grading (P < 0.05), and there are differences among different inflammatory phenotypes (P < 0.05): Non eo-sinophilic phenotype > eosinophilic phenotype. Conclusion: Levels of IL-6 and IL-8 in induced spu-tum of COPD patients correlated with acute exacerbations in COPD patients, positively correlated with gold grade, and have lower expression in the eosinophilic phenotype than in the non eosino-philic phenotype.
文章引用：史秀枝, 曹晓红. COPD患者诱导痰中IL-6、IL-8水平与炎症表型的相关性研究[J]. 临床医学进展, 2022, 12(5): 4287-4294. https://doi.org/10.12677/ACM.2022.125621

参考文献

[1] Global Initiative for Chronic Obstructive Lung Disease (2019) 2020 Global Strategy for the Diagnosis, Management and Prevention of Chronic Obstructive Lung Disease 2020 Report. Global Initiative for Chronic Obstructive Lung Disease.
https://goldcopd.org/gold-reports/
[2] Zatloukal, J., Brat, K., Neumannova, K., Volakova, E., Hejduk, K., Kocova, E., et al. (2020) Chronic Obstructive Pulmonary Disease-Diagnosis and Management of Stable Disease: A Personalized Approach to Care, Using the Treatable Traits Concept Based on Clinical Phenotypes. Position Paper of the Czech Pneu-mological and Phthisiological Society, Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub, 164, 325-356.
[3] Gao, P., Zhang, J., He, X., Hao, Y., Wang, K. and Gibson, P.G. (2013) Sputum Inflammatory Cell-Based Classification of Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease. PLoS ONE, 8, Article ID: e57678. [Google Scholar] [CrossRef] [PubMed]
[4] Hegewald, M.J., Horne, B.D., Trudo, F., Kreindler, J.L., Chung, Y., Rea, S., et al. (2020) Blood Eosinophil Count and Hospital Readmission in Patients with Acute Exacerbation of Chronic Obstructive Pulmonary Disease. International Journal of Chronic Obstructive Pulmonary Disease, 15, 2629-2641. [Google Scholar] [CrossRef] 
[5] Southworth, T., Higham, A., Kolsum, U., Li, J., Scott, T., Dungwa, J., et al. (2021) The Relationship between Airway Immunoglobulin Activity and Eosinophils in COPD. Journal of Cellular and Molecular Medicine, 25, 2203-2212. [Google Scholar] [CrossRef] [PubMed]
[6] 于春歌, 华毛. AECOPD患者炎症表型及炎性标志物研究进展[J]. 临床肺科杂志, 2018, 23(10): 1887-1890.
[7] Guo, X.X., Deng, N.S., Chen, Q.H., Yu, H.Y., Ding, X.H., Hu, S.P., et al. (2019) Application of Inflammatory Markers in Induced Sputum in Stable Chronic Obstructive Pulmonary Disease Patients with Positive Bronchodilation Tests. Current Medical Science, 39, 560-567. [Google Scholar] [CrossRef] [PubMed]
[8] Global Initiative for Chronic Obstructive Lung Disease (2018) Global Strategy for the Diagnosis, Management, and Prevention of Chronic Obstructive Pulmonary Disease: 2018 Report. Global Initiative for Chronic Obstructive Lung Disease.
https://goldcopd.org/
[9] Garudadri, S. and Woodruff, P.G. (2018) Targeting Chronic Obstructive Pulmonary Disease Phenotypes, Endotypes, and Biomarkers. Annals of the American Thoracic Society, 15, S234-S238. [Google Scholar] [CrossRef] 
[10] Bakeer, M., Funk, G.C. and Valipour, A. (2020) Chronic Obstructive Pulmonary Disease Phenotypes: Imprint on Pharmacological and Non-Pharmacological Therapy. Annals of Translational Medicine, 8, 1472. [Google Scholar] [CrossRef] [PubMed]
[11] Crespo-Lessmann, A., Plaza, V. and Consensus Group (2020) Multi-disciplinary Consensus on Sputum Induction Biosafety during the COVID-19 Pandemic. Allergy, 76, 2407-2419. [Google Scholar] [CrossRef] [PubMed]
[12] De Nijs, S.B., Fens, N., Lutter, R., Dijkers, E., Krouwels, F.H., Smids-Dierdorp, B.S., et al. (2011) Airway Inflammation and Mannitol Challenge Test in COPD. Respiratory Research, 12, Article No. 11. [Google Scholar] [CrossRef] [PubMed]
[13] 刘超, 王伟. 慢性阻塞性肺疾病患者诱导痰中正五聚蛋白3水平的变化及意义[J]. 中国老年学杂志, 2014(24): 6951-6952.
[14] Zhu, T., Li, S., Wang, J., Liu, C., Gao, L., Zeng, Y., et al. (2020) Induced Sputum Metabolomic Profiles and Oxidative Stress Are Associated with Chronic Obstructive Pul-monary Disease (COPD) Severity: Potential Use for Predictive, Preventive, and Personalized Medicine. EPMA Journal, 11, 645-659. [Google Scholar] [CrossRef] [PubMed]
[15] Lai, T., Xiang, F., Zeng, J., Huang, Y., Jia, L., Chen, H., et al. (2020) Reliability of Induced Sputum Test Is Greater Than That of Throat Swab Test for Detecting SARS-CoV-2 in Patients with COVID-19: A Multi-Center Cross-Sectional Study. Virulence, 11, 1394-1401. [Google Scholar] [CrossRef] [PubMed]
[16] Davoine, F. and Lacy, P. (2014) Eosinophil Cytokines, Chemokines, and Growth Factors: Emerging Roles in Immunity. Frontiers in Immunology, 5, Article No. 570. [Google Scholar] [CrossRef] [PubMed]
[17] Bradford, E., Jacobson, S., Varasteh, J., Comellas, A.P., Wood-ruff, P., O’Neal, W., et al. (2017) The Value of Blood Cytokines and Chemokines in Assessing COPD. Respiratory Re-search, 18, Article No. 180. [Google Scholar] [CrossRef] [PubMed]
[18] 杜伟. IL-8的变化在慢阻肺患者血清及支气管肺泡灌洗液中的应用价值研究[J]. 中外医学研究, 2018, 16(23): 14-16.
[19] Aslani, M.R., Ghazaei, Z. and Ghobadi, H. (2020) Correlation of Serum Fatty Acid Binding Protein-4 and Interleukin-6 with Airflow Limitation and Quality of Life in Sta-ble and Acute Exacerbation of COPD. Turkish Journal of Medical Sciences, 50, 337-345. [Google Scholar] [CrossRef] [PubMed]
[20] Gorska, K., Nejman-Gryz, P., Paplinska-Goryca, M., Korczynski, P., Prochorec-Sobieszek, M. and Krenke, R. (2018) Comparative Study of IL-33 and IL-6 Levels in Different Respiratory Samples in Mild-to-Moderate Asthma and COPD. COPD, 15, 36-45. [Google Scholar] [CrossRef] [PubMed]
[21] 王胜, 徐风珍, 陈余清. 慢性阻塞性肺疾病痰液白细胞介素8、6和肿瘤坏死因子测定及其意义[J]. 中华结核和呼吸杂志, 2000, 23(8): 465-467.
[22] Grubek-Jaworska, H., Paplińska, M., Hermanowicz-Salamon, J., Białek-Gosk, K., Dąbrowska, M., Grabczak, E., et al. (2012) IL-6 and IL-13 in Induced Sputum of COPD and Asthma Patients: Correlation with Respiratory Tests. Respiration, 84, 101-107. [Google Scholar] [CrossRef] [PubMed]
[23] Córdoba-Lanús, E., Baz-Dávila, R., Espinoza-Jiménez, A., Rodríguez-Pérez, M.C., Varo, N., de-Torres, J.P., et al. (2015) IL-8 Gene Variants Are Associated with Lung Function Decline and Multidimensional BODE Index in COPD Patients but Not with Disease Susceptibility: A Validation Study. COPD, 12, 55-61. [Google Scholar] [CrossRef] [PubMed]
[24] Zhang, J., Feng, M.X. and Qu, J.M. (2012) Low Dose The-ophylline Showed An Inhibitory Effect on the Production of IL-6 and IL-8 in Primary Lung Fibroblast From Patients with COPD. Mediators of Inflammation, 2012, Article ID: 492901. [Google Scholar] [CrossRef] [PubMed]
[25] Qi, Y.J., Sun, X.J., Wang, Z., Bin, Y.-F., Li, Y.-H., Zhong, X.-N., et al. (2020) Richness of Sputum Microbiome in Acute Exacerbations of Eosinophilic Chronic Obstructive Pulmonary Disease. Chinese Medical Journal, 133, 542-551. [Google Scholar] [CrossRef] 
[26] Kume, H., Hojo, M. and Hashimoto, N. (2019) Eosinophil Inflammation and Hyperresponsiveness in the Airways as Phenotypes of COPD, and Usefulness of Inhaled Glucocorti-costeroids. Frontiers in Pharmacology, 10, Article No. 765. [Google Scholar] [CrossRef] [PubMed]
[27] Lv, M.Y., Qiang, L.X., Li, Z.H. and Jin, S.-D. (2021) The Lower the Eosinophils, the Stronger the Inflammatory Response? The Relationship of Different Levels of Eosinophils with the Degree of Inflammation in Acute Exacerbation Chronic Obstructive Pulmonary Disease (AECOPD). Journal of Thoracic Disease, 13, 232-243. [Google Scholar] [CrossRef] [PubMed]
[28] Hastie, A.T., Martinez, F.J., Curtis, J.L., et al. (2017) Association of Sputum and Blood Eosinophil Concentrations with Clinical Measures of COPD Severity: An Analysis of the SPIROMICS Cohort. Lancet Respir Med, 5, 956-967. [Google Scholar] [CrossRef] 
[29] Hartley, B.F., Barnes, N.C., Lettis, S., Compton, C.H., Papi, A., Jones, P., et al. (2020) Risk Factors for Exacerbations and Pneumonia in Patients with Chronic Obstructive Pulmo-nary Disease: A Pooled Analysis. Respiratory Research, 21, Article No. 5. [Google Scholar] [CrossRef] [PubMed]

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