含硫生物分子的检测及成像技术的研究进展
Recent Advances in Detection, Isolation, and Imaging Techniques for Sulfane Sulfur-Containing Biomolecules
DOI: 10.12677/AAC.2022.122019, PDF,    科研立项经费支持
作者: 夏 超, 丁月旭*：重庆市公共卫生医疗救治中心，重庆
关键词: 含硫生物分子；检测技术；生物成像；Sulfane Sulfur-Containing Biomolecules； Detection Techniques； Bioimaging
摘要: 硫化氢(H2S)及其氧化产物在许多生物过程中都具有重要的作用，一些含硫生物分子如R-S-SH、R-S-Sn-S-R、H2Sn等，引起了人们的广泛关注。为描述它们在生理和病理生理中的作用，需要有选择性的检测技术。传统上的氰解法可用来检测含硫生物分子，但它在生物成像方面作用有限。随着各种化学检测技术不断发展，Tag-Switch技术、LC-MS/MS、拉曼光谱和荧光探针等技术均已用于检测含硫生物分子及其生物成像。为此，我们将基于这些技术对含硫生物分子的检测、成像的研究进展进行综述，以期为后续设计开发更具实用价值的检测技术提供有益的参考。
Abstract: Hydrogen sulfide and its oxidation products are involved in many biological processes, and sulfane sulfur compounds, which contain sulfur atoms bonded to other sulfur atom(s), as found in hydropersulfides (R-S-SH), polysulfides (R-S-Sn-S-R), hydrogen polysulfides (H2Sn), etc., have attracted increasing interest. To characterize their physiological and pathophysiological roles, selective detection techniques are required. Classically, sulfane sulfur compounds can be detected by cyanolysis, but it is useful in biological imaging. Tag-switch techniques, LC-MS/MS, Raman spectroscopy, and fluorescent probes have been used to detect Sulfane Sulfur-Containing Biomolecules and their bioimaging. Herein, this review summarizes the techniques available for specific detection of sulfane sulfur species in biological contexts, in order to provide a useful reference for the subsequent design and development of more practical detection technology.
文章引用：夏超, 丁月旭. 含硫生物分子的检测及成像技术的研究进展[J]. 分析化学进展, 2022, 12(2): 149-156. https://doi.org/10.12677/AAC.2022.122019

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