基于网络药理学研究升陷汤治疗肺纤维化的作用机制

The Mechanisms of Sheng Xian Tang against Pulmonary Fibrosis: A Network Pharmacology-Based Study

摘要: 目的：为了挖掘升陷汤的有效成分、预测其潜在功能靶标，通过网络药理学探讨升陷汤治疗肺纤维化的作用机制。方法：利用TCMSP数据库筛选升陷汤活性成分及其作用的潜在靶点；通过GeneCards数据库检索与肺纤维化疾病相关的靶点；将疾病相关靶点映射到化合物潜在靶点中，获取药物–疾病共同靶点，并将信息导入Cytoscape4.7.0软件和STRING在线分析平台分别制作网络图和PPI图，再进行KEGG和GO富集分析。结果：共筛选出69个活性成分以及101个关键靶点，涉及PI3K-Akt signaling pathway、Fluid shear stress and atherosclerosis、AGE-RAGE signaling pathway in diabetic complications等信号通路。结论：网络药理学研究揭示升陷汤可通过多成分–多靶点–多途径共同调控肺纤维化的物质基础和作用机制，为升陷汤的临床应用提供了理论基础和科学依据。

Abstract: Background: To investigate the main compounds, the underlying functional targets and the me- chanism of Sheng Xian Tang (upraise the sunken decoction, SXT) formula treating pulmonary fibrosis (PF) through network pharmacology analysis. Methods: The main compounds and targets of SXT were mainly collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP). PF relating targets were collected from the GeneCards databases. Subsequently, targets were mapped into the compounds to obtain a compound-disease complex, and then employ STRING online analysis platform and Cytoscape4.7.0 software to generate protein-protein interaction (PPI) networks. Then the related pathways and correlation analysis were explored by the Kyoto Encyclopedia and Genomes (KEGG) and Gene Ontology (GO) analysis. Results: The database results showed that there were 69 active compounds in SXT, and 101 targets were screened out for PF treatment. Network analysis indicated that the main targets of the main active components of SXT were target genes such as PI3K-Akt, Fluid shear stress and atherosclerosis, and AGE-RAGE signaling pathways. PI3K-Akt signaling pathway, Fluid shear stress and atherosclerosis, AGE-RAGE signaling pathway in diabetic complications and HIF-1 signaling pathway, etc. Conclusions: This study helped to validate the material basis and underlying mechanism of SXT for treating PF in a multi-components, multi-targets, and multi-pathways pattern. Additionally, this study provides a theoretical basis and scientific proof for the reasonable application of SXT in the clinical practice for PF.