叶酸快速检测方法及电化学分析综述
A Review for Rapid Detection Methods and Electrochemical Analysis of Folic Acid
DOI: 10.12677/AAC.2022.122020, PDF,    科研立项经费支持
作者: 李 浩, 林珍富, 何文畅, 陈柳君, 陈悦欣, 冯小珍*：桂林电子科技大学生命与环境科学学院，广西 桂林
关键词: 叶酸；检测方法；快速检测；电化学分析；Folic Acid； Detection Methods； Rapid Detection； Electrochemical Analysis
摘要: 叶酸(Folic acid)是人体不可少的维生素，体液中叶酸浓度的降低会导致多种疾病。目前叶酸的检测方法种类繁多，包括微生物法、比色法、高效液相色谱法、荧光法、薄层层析法、离子捕获法、紫外分光光度法和电化学分析法等，本文对主要的快速检测方法进行了综述。其中较为主流的就是微生物法和高效液相色谱法，其简单易行且灵敏度高，非常适合低质量分数的叶酸检测。而在实验室的研究中，电化学分析由于可以在电脑上快速清楚地呈现电化学反应，非常便于分析其线性关系，同时它还具有高灵敏度、低成本和操作简便等优点，所以更加适用。另外，电化学方法还可以深层次研究叶酸的反应机理和动力学参数，因而有很大的发展潜力。
Abstract: Folic acid (FA) is an indispensable vitamin in human body. The reduction of FA concentration in body fluid will lead to a variety of diseases. At present, there are many detection methods of FA, including microbial method, high performance liquid chromatography (HPLC), colorimetry, fluorescence, thin layer chromatography, ion capture, ultraviolet spectrophotometry and electrochemical analysis. In this paper, we summarized the main rapid detection methods. Among them, microbial method and HPLC are the mainstream because they are simple, sensitive and very suitable for the detection of FA with low mass fraction. In laboratory research, however, electrochemical analysis is more suitable because it can quickly and clearly show the electrochemical reaction of FA on a computer, which is very convenient to analyze the linear relationship. Meanwhile, it also has the advantages of high sensitivity, low cost and simple operation. In addition, various methods of electrochemical analysis can also deeply study the reaction mechanism and kinetic parameters of FA, indicating great potential.
文章引用：李浩, 林珍富, 何文畅, 陈柳君, 陈悦欣, 冯小珍. 叶酸快速检测方法及电化学分析综述[J]. 分析化学进展, 2022, 12(2): 157-164. https://doi.org/10.12677/AAC.2022.122020

参考文献

[1] 陈伟才, 李忠信, 渠海, 付光宇. 叶酸及维生素B12检测方法的发展及现状[J]. 分子诊断与治疗杂志, 2015, 7(5): 357-360+356.
[2] 杨玉柱, 王储炎, 焦必宁. 叶酸的研究进展[J]. 农产品加工学刊, 2006(5): 31-35+39.
[3] Angier, R.B., Boothe, J.H., Hutchings, B.L., Mowat, J.H., Semb, J., Stokstad, E.L., et al. (1946) The Structure and Synthesis of the Liver L. casei Factor. Science, 103, 667-669.
[Google Scholar] [CrossRef] [PubMed]
[4] 倪青, 曹岸江, 主编. 常用临床药物手册[M]. 北京: 中国医药科技出版社, 1999: 169.
[5] 方尚玲. 叶酸及其检测[J]. 武汉工业学院学报, 2000(1): 8-11.
[6] Birn, H., Selhub, J. and Christensen, E.I. (1993) Internalization and Intracellular Transport of Folate-Binding Protein in Rat Kidney Proximal Tubule. American Journal of Physiology: Cell Physiology, 264, C302-C310.
[Google Scholar] [CrossRef] 
[7] 王翔朴, 王营通, 李珏声, 主编. 卫生学大辞典[M]. 青岛: 青岛出版社, 2000: 844-844.
[8] 王博伦, 顾丰颖, 刘子毅, 张帆, 刘昊, 杨婷婷, 等. 食品中叶酸测定方法的研究进展[J]. 食品科学, 2020, 41(9): 294-300.
[9] 陈朴, 潘柏申. 血清叶酸和红细胞叶酸检测的临床应用[J]. 检验医学, 2016, 31(3): 232-236.
[10] O’Broin, S. and Kelleher, B. (1992) Microbiological Assay on Microtitre Plates of Folate in Serum and Red Cells. Journal of Clinical Pathology, 45, 344-347.
[Google Scholar] [CrossRef] [PubMed]
[11] Tamura, T. and Picciano, M.F. (2006) Folate and Human Reproduction. The American Journal of Clinical Nutrition, 83, 993-1016.
[Google Scholar] [CrossRef] [PubMed]
[12] 黄新河, 莫宏春, 刘克武. 叶酸及其应用[J]. 四川食品与发酵, 2003(4): 27-30.
[13] 中华人民共和国国家卫生和计划生育委员会. GB5009 211-2014食品安全国家标准 食品中叶酸的测定[S]. 北京: 中国标准出版社, 2015.
[14] Han, J.Y. and Tyler, R.T. (2003) Determination of Folate Concentrations in Pulses by a Microbiological Method Employing Trienzyme Extraction. Journal of Agricultural and Food Chemistry, 51, 5315-5318.
[Google Scholar] [CrossRef] [PubMed]
[15] Spray, G.H. (1964) Microbiological Assay of Folic Acid Activity in Human Serum. Journal of Clinical Pathology, 17, 660-665.
[Google Scholar] [CrossRef] [PubMed]
[16] 汪锦邦, 顾鹏, 章德宏, 李东. 叶酸分析方法的研究进展[J]. 中国食品添加剂, 2000(3): 49-54.
[17] Englehardt, R. and Gregory, J.F. (1990) Adequacy of Enzymatic Deconjugation in Quantification of Folate in Foods. Journal of Studies in Education, 38, 154-158.
[Google Scholar] [CrossRef] 
[18] 任一平, 张爱珍, 朱黎炎, 黄百芬, 铁晓威. 高效液相色谱法测定食品中的叶酸[J]. 食品与发酵工业, 2003(10): 46-49.
[19] 张春燕, 顾健. 反相高效液相色谱法同时测定人血浆中亚叶酸、5-甲基四氢叶酸及甲氨蝶呤的浓度及临床应用[J]. 中国药学杂志, 2010, 45(7): 543-547.
[20] 中华人民共和国农业部. NY/T 2895-2016饲料中叶酸的测定 高效液相色谱法[S]. 北京: 中国标准出版社, 2016.
[21] 中华人民共和国工业和信息化部. QB/T 5346-2018口腔清洁护理用品 牙膏中叶酸含量的测定 高效液相色谱法[S]. 北京:中国标准出版社, 2018.
[22] 刘欣, 黄汉国. 高锰酸钾氧化-荧光分光光度法测定片剂中叶酸含量[J]. 分析化学, 2000, 28(11): 1406-1409.
[23] 刘欣, 黄汉国. 过氧化氢氧化-荧光分光光度法测定片剂中叶酸含量[J]. 分析化学, 2002, 30(8): 1018.
[24] 刘欣, 黄汉国. 叶酸氧化产物体系荧光法测定铕[J]. 河北化工, 2004(3): 62-64.
[25] 吴琼, 孙洋, 谷伟, 钱方. 薄层层析法检测乳糖水解产物的研究[J]. 食品与机械, 2011, 27(6): 135-136+156.
[26] 石丹, 贾云虹, 包怡红, 生庆海. 叶酸检测方法的研究现状及发展趋势[J]. 中国乳品工业, 2009, 37(3): 42-45.
[27] Wilson, D.H., Hermamn, R., Hsu, S., Biegalski, T., Sohn, L., Forsythe, C., et al. (1995) Iron Capture Assay for Folate with the Abbott IMx Analyzer. Clinical Chemistry, 41, 1780-1781.
[Google Scholar] [CrossRef] 
[28] 陈懿. 分光光度法测定叶酸含量[J]. 医药工业, 1987(7): 327-328.
[29] 国家市场监督管理总局, 中国国家标准化管理委员会. GB 7302-2018饲料添加剂: 叶酸[S]. 北京: 中国标准出版社, 2018.
[30] Park, S., Chung, T.D. and Kim, H.C. (2003) Nonenzymatic Glucose Detection Using Mesoporous Platinum. Analytical Chemistry, 75, 3046-3049.
[Google Scholar] [CrossRef] [PubMed]
[31] 王丁泉. 放射免疫分析发展历史和建议[J]. 同位素, 2019, 32(3): 204-207.
[32] Osganian, S.K., Stampfer, M.J., Spiegelman, D., Rimm, E., Cutler, J.A., Feldman, H.A., et al. (1999) Distribution of and Factors Associated with Serum Omocysteine Levels in Children. JAMA, 281, 1189-1196.
[Google Scholar] [CrossRef] [PubMed]
[33] Ford, E.S. and Bowman, B.A. (1999) Serum and Red Blood Cell Folate Concentrations, Race, and Education: Findings from the Third National Health and Nutrition Examination Survey. The American Journal of Clinical Nutrition, 69, 476-481.
[Google Scholar] [CrossRef] [PubMed]
[34] 沈国松, 何平亚, 张甦, 卢宝庭, 薛建英. 早孕妇女红细胞叶酸含量测定及其临床意义[J]. 浙江医学, 2007, 29(8): 814-815.
[35] Newman, E.M. and Tsai, J.F. (1986) Microbiological Analysis of 5-Formyltetrahydrofolic Acid and Other Folates Using an Automatic 96-Well Plate Reader. Analytical Biochemistry, 154, 509-515.
[Google Scholar] [CrossRef] [PubMed]
[36] 范松阳. 微孔板法测定乳粉中叶酸的含量[D]: [硕士学位论文]. 哈尔滨: 东北农业大学食品加工与安全系, 2018.
[37] Cheng Y.-Y., Zhan T., Feng X.-Z. and Han, G.-C. (2021) A Synergistic Effect of Gold Nanoparticles and Melamine with Signal Amplification for C-Reactive Protein Sensing. Journal of Electroanalytical Chemistry, 895, Article ID: 115417.
[Google Scholar] [CrossRef] 
[38] Han G.-C., Su X., Hou J., Ferranco, A., Feng, X.-Z., Zeng, R., et al. (2019) Disposable electrochemical sensors for hemoglobin detection based on ferrocenoyl cysteine conjugates modified electrode. Sensors and Actuators B: Chemical, 282, 130-136.
[Google Scholar] [CrossRef] 
[39] Zhang, D., Yang, X., Ma, W., Li, L. and Zhang, Y. (2016) Voltammetric Determination of Folic Acid Using Adsorption of Methylene Blue onto Electrodeposited of Reduced Graphene Oxide Film Modified Glassy Carbon Electrode. Electroanalysis, 28, 312-319.
[Google Scholar] [CrossRef] 
[40] Manoj, D., Ranjith, K.D. and Santhanalakshmi, J. (2012) Impact of CuO nanoleaves on MWCNTs/GCE Nanocomposite Film Modified Electrode for the Electrochemical Oxidation of Folic Acid. Applied Nanoscience, 2, 223-230.
[Google Scholar] [CrossRef] 
[41] An, Q.-Q., Feng, X.-Z., Zhou, Z.-F., Zhan, T., Lian, S.-F., Zhu, J., et al. (2022) One Step Construction of an Electrochemical Sensor for Melamine Detection in Milk towards an Integrated Portable System. Food Chemistry, 383, Article ID: 132403.
[Google Scholar] [CrossRef] [PubMed]
[42] Ye, H., Song, L., Zhang, F., Li, J., Su, Z. and Zhang, Y. (2021) Highly Sensitive Electrochemical Detection of Folic Acid by Using a Hollow Carbon Nanospheres@molybdenum Disulfide Modified Electrode. Analytical Sciences, 37, 575-580.
[Google Scholar] [CrossRef] 
[43] Garcia, S.M., Wong, A., Khan, S. and Sotomayor, M.D.P.T. (2021) A Simple, Sensitive and Efficient Electrochemical Platform Based on Carbon Paste Electrode Modified with Fe3O4@MIP and Graphene Oxide for Folic Acid Determination in Different Matrices. Talanta, 229, Article ID: 122258.
[Google Scholar] [CrossRef] [PubMed]
[44] Zhan T., Feng X.-Z., An Q.-Q., Li, S., Xue, M., Chen, Z., et al. (2022) Enzyme-Free Glucose Sensors with Efficient Synergistic Electro-Catalysis Based on a Ferrocene Derivative and Two Metal Nanoparticles. RSC Advances, 12, 5072-5079.
[Google Scholar] [CrossRef] 
[45] 任超超, 孙玉琴, 高作宁. 叶酸在多壁碳纳米管修饰玻碳电极上的电催化氧化及其电分析方法[J]. 宁夏大学学报(自然科学版), 2010, 31(1): 70-73.

为你推荐