猕猴桃溃疡病菌侵染过程中鞭毛基因的转录组表达分析

Transcriptome Expression Analysis of Flagella Gene during Infection of Pseudomonas syringae pv. actinidiae

摘要: 为了发掘猕猴桃溃疡病菌的致病相关基因，利用(RNA sequencing, RNA-Seq)技术对接菌不同时间点的猕猴桃与溃疡病菌进行互作转录组测序分析。结果表明：共获得101.01 Gb Clean Data，各样品Clean Data均达到4.85 Gb以上，Q30碱基百分比在96.77%以上。其中与鞭毛相关并表达的差异基因有31个。鞭毛相关差异表达基因GO注释表明参与细胞过程的基因数目最多，细胞组分和分子功能次之；鞭毛相关差异表达基因KEGG通路分析表明差异基因的注释以细胞运动为主。经qRT-PCR检测表明，5个随机选择的差异表达基因表达量变化与转录组测序结果基本一致。本研究结果对猕猴桃溃疡病致病基因发掘具有重要的理论指导和实践意义。

Abstract: In order to explore the pathogenic genes of Pseudomonas syringae pv. actinidiaer, use (RNA sequencing, RNA-Seq) transcriptome sequencing analysis of interaction between kiwifruit and Psa at different time points of technology docking bacteria. The results show that: a total of 101.01 Gb of clean data were obtained, the clean data of each sample reached more than 4.85 Gb, and the base percentage of Q30 was more than 96.77%. Among them, there are 31 differentially expressed genes related to flagella. GO annotation of flagella-related differentially expressed genes showed that the number of genes involved in cell process was the largest, followed by cell components and molecular functions. KEGG pathway analysis of flagella-related differentially expressed genes showed that the enrichment of differential genes was mainly cell movement. qRT-PCR showed that the expression changes of five randomly selected differentially expressed genes were consistent with the transcriptome sequencing results. The results of this study have important theoretical guidance and practical significance for discovering the pathogenic genes of kiwifruit canker.