混合价态铂纳米颗粒对持留菌杀伤作用的探索

Exploration of the Lethal Effects of the Dual-Valent Platinum Nanoparticles on Persisters

摘要: 目前有关抗菌方法的研究已经取得了丰硕的成果，而本项目重点在于联合多种抗菌机制进行高效、准确杀菌，新型混合价态铂纳米颗粒(dvPtNPs)则是研究的主要对象。本材料以零价铂核以及金属键结合的二价铂离子为核心，外由有机聚合物包裹，在近红外光(NIR)照射下，dvPtNPs快速释放出二价铂离子，发挥光热、光动力学以及化疗的多机制协同的精准可控抗菌作用。在之前研究的基础上，本项目发现dvPtNPs对于持留菌也有很好的杀伤作用，并通过多种方法探究了其杀伤持留菌的机制，包括使细菌RNA泄露、降低膜电位和ATP水平等。该材料有望成为对抗持留菌感染的新型治疗方法。

Abstract: Current academic research on antibacterial methods has been fruitful, and our project focuses on efficient and accurate sterilization by combining multiple antibacterial mechanisms. The dual-valent platinum nanoparticles (dvPtNPs) are regarded as the main research object. The core of dvPtNPs is the central zero-valent platinum core and the wrapped divalent platinum ion, and is covered by organic polymer. Under near-infrared light (NIR) irradiation, it rapidly releases the divalent platinum ion, together with the zero-valent platinum core, exerts precise and controllable antibacterial effects integrating multiple mechanisms including photothermal, photodynamic and chemotherapy. On the basis of previous studies, this project found that dvPtNPs also have a good killing effect on persisters through various mechanisms, including causing bacterial RNA leakage, reducin membrane potential and ATP level, etc. This material is expected to be a new treatment method against persistent bacterial infection.