一株游泳池水中沃氏葡萄球菌的分离鉴定与耐药性分析

Isolation, Identification and Drug Resistance Analysis of a Strain of Staphylococcus warneri in Swimming Pool Water

摘要: 目的：了解游泳池水中可能存在的病原菌，预防和控制游泳池相关疾病的传播流行。方法：利用对葡萄球菌有选择性的7.5%氯化钠肉汤和Baird-Parker (BP)琼脂平板培养基分离游泳池水中的细菌，初步鉴定采用革兰染色和血浆凝固酶实验，进一步鉴定采用法国梅里埃公司的Vitek2 compact全自动微生物鉴定和药敏分析系统。结果：该菌为血浆凝固酶阴性的葡萄球菌，具有沃氏葡萄球菌的生化特征，诱导性克林霉素耐药阳性，显示对红霉素和克林霉素耐药，头孢西丁筛选实验阴性，对其它抗生素均敏感。结论：在攀枝花市游泳池水中检测出一株沃氏葡萄球菌，鉴于游泳池的水质状况关系到人类的身体健康，应加强对游泳池水中致病菌及其耐药性的监测和研究。

Abstract: Objective: To understand the possible pathogenic bacteria in swimming pool water and to prevent and control the spread of swimming pool related diseases. Methods: The bacteria in the swimming pool were isolated by staphylococcal selective 7.5% sodium chloride broth and Baird-Parker (BP) plate culture medium. The bacteria were preliminarily identified by gram staining and plasma coagulase experiment, and further identified by Vitek2 compact automatic microorganism identification and drug sensitivity analysis system of French Merrier Company. Results: The bacteria was staphylococcus with negative plasma coagulase, which had the biochemical characteristics of S. warneri and positive induced clindamycin resistance, indicating resistance to erythromycin and clindamycin, and cefoxitin screening test was negative and sensitive to other antibiotics. Conclusion: A strain of S. warneri was detected in the swimming pool water of Panzhihua city. In view of that the water quality of the swimming pool is related to human health, the monitoring and research on pathogenic bacteria and their resistance in the swimming pool water should be strengthened.