SREBF2-AS1调控胆固醇合成代谢促进乳腺癌生长
SREBF2-AS1 Promotes the Growth of Breast Cancer via Regulating Cholesterol Anabolism
DOI: 10.12677/WJCR.2022.123016, PDF,    国家自然科学基金支持
作者: 邵 玲, 杨宜英, 洪宏海:广州医科大学附属第三医院,广东 广州
关键词: 乳腺癌生长SREBF2-AS1胆固醇合成代谢Breast Cancer Growth SREBF2-AS1 Cholesterol Metabolism
摘要: 目的:研究SREBF2-AS1调控胆固醇合成代谢和SREBF2促进乳腺癌生长作用和分子机制。方法:qRT-PCR检测相关基因的转录水平。干扰SREBF2-AS1和过表达SREBF2后,MTT实验、BrdU实验和凋亡实验研究SREBF2-AS1在乳腺癌中作用及分子机制。结果:SREBF2-AS1在乳腺癌组织和乳腺癌细胞株中高表达。干扰掉SREBF2-AS1后抑制乳腺癌的生长和诱导其凋亡。进一步研究发现其通过调控SREBF2和胆固醇代谢进而调控乳腺癌生长。结论:SREBF2-AS1通过调控SREBF2和胆固醇代谢促进乳腺癌生长。
Abstract: Objective: To study the molecular mechanism of SREBF2-AS1 regulating cholesterol anabolism and SREBF2 promoting the growth of breast cancer. Methods: The transcription level of Genes was detected by qRT PCR. After interference with SREBF2-AS1 and overexpression of SREBF2, MTT assay, BrdU assay and apoptosis assay were used to study the role and molecular mechanism of SREBF2- AS1 in breast cancer. Results: SREBF2-AS1 was highly expressed in breast cancer tissues and breast cell lines. Interference with SREBF2-AS1 inhibits the growth and induces apoptosis of breast cancer. Further studies found that it regulates the growth of breast cancer by regulating SREBF2 and cholesterol metabolism. Conclusion: SREBF2-AS1 can promote the growth of breast cancer by regulating SREBF2 and cholesterol metabolism.
文章引用:邵玲, 杨宜英, 洪宏海. SREBF2-AS1调控胆固醇合成代谢促进乳腺癌生长[J]. 世界肿瘤研究, 2022, 12(3): 117-123. https://doi.org/10.12677/WJCR.2022.123016

参考文献

[1] Desantis, C., Ma, J., Bryan, L. and Jemal, A. (2014) Breast Cancer Statistics, 2013. CA: A Cancer Journal for Clinicians, 64, 52-62.
[Google Scholar] [CrossRef] [PubMed]
[2] Horton, J.K., Jagsi, R., Woodward, W.A. and Ho, A. (2018) Breast Cancer Biology: Clinical Implications for Breast Radiation Therapy. International Journal of Radiation Oncology, Biology, Physics, 100, 23-37.
[Google Scholar] [CrossRef] [PubMed]
[3] Fontes-Sousa, M., Amorim, M., Salta, S., Palma, D.S.S., Henrique, R. and Jeronimo, C. (2019) Predicting Resistance to Endocrine Therapy in Breast Cancer: It’s Time for Epigenetic Biomarkers (Review). Oncology Reports, 41, 1431-1438.
[Google Scholar] [CrossRef] [PubMed]
[4] Mercer, T.R., Dinger, M.E. and Mattick, J.S. (2009) Long Non-Coding RNAs: Insights into Functions. Nature Reviews Genetics, 10, 155-159.
[Google Scholar] [CrossRef] [PubMed]
[5] Kang, M.J., Abdelmohsen, K., Hutchison, E.R., et al. (2014) HuD Regulates Coding and Noncoding RNA to Induce APP → Aβ Processing. Cell Reports, 7, 1401-1409.
[Google Scholar] [CrossRef] [PubMed]
[6] Mendell, J.T. (2016) Targeting a Long Noncoding RNA in Breast Cancer. The New England Journal of Medicine, 374, 2287-2289.
[Google Scholar] [CrossRef
[7] Touvier, M., Fassier, P., His, M., et al. (2015) Cholesterol and Breast Cancer Risk: A Systematic Review and Meta-Analysis of Prospective Studies. British Journal of Nutrition, 114, 347-357.
[Google Scholar] [CrossRef
[8] Kitahara, C.M., Berrington, D.G.A., Freedman, N.D., et al. (2011) Total Cholesterol and Cancer Risk in a Large Prospective Study in Korea. Journal of Clinical Oncology, 29, 1592-1598.
[Google Scholar] [CrossRef
[9] Melvin, J.C., Seth, D., Holmberg, L., et al. (2012) Lipid Profiles and Risk of Breast and Ovarian Cancer in the Swedish AMORIS Study. Cancer Epidemiology, Biomarkers & Prevention, 21, 1381-1384.
[Google Scholar] [CrossRef
[10] Goldstein, J.L., Debose-Boyd, R.A. and Brown, M.S. (2006) Protein Sensors for Membrane Sterols. Cell, 124, 35-46.
[Google Scholar] [CrossRef] [PubMed]
[11] Borgquist, S., Giobbie-Hurder, A., Ahern, T.P., et al. (2017) Cholesterol, Cholesterol-Lowering Medication Use, and Breast Cancer Outcome in the BIG 1-98 Study. Journal of Clinical Oncology, 35, 1179-1188.
[Google Scholar] [CrossRef
[12] Horton, J.D., Shimomura, I., Brown, M.S., Hammer, R.E., Goldstein, J.L. and Shimano, H. (1998) Activation of Cholesterol Synthesis in Preference to Fatty Acid Synthesis in Liver and Adipose Tissue of Transgenic Mice Overproducing Sterol Regulatory Element-Binding Protein-2. Journal of Clinical Investigation, 101, 2331-2339.
[Google Scholar] [CrossRef
[13] Shimano, H., Shimomura, I., Hammer, R.E., et al. (1997) Elevated Levels of SREBP-2 and Cholesterol Synthesis in Livers of Mice Homozygous for a Targeted Disruption of the SREBP-1 Gene. Journal of Clinical Investigation, 100, 2115-2124.
[Google Scholar] [CrossRef