不同试剂对多花黄精种子萌发和生长的影响

Effects of Different Reagents on Germination and Growth of Polygonatum cyrtonema Seeds

摘要: 目的：旨在解决多花黄精有性繁殖育苗的种子休眠问题，研究不同试剂处理及浸种时间对多花黄精种子萌发的影响。方法：将采回的多花黄精种子用钨酸钠(588、1176、1764、2352 mg/L)和氟啶酮(5、10、15、20 mg/L)两种试剂，分别与浸种时间(12 h、18 h、24 h)组合处理，用无菌水处理作为对照，70 d后对种子发芽率、发芽势、烂种率以及初生根茎芽长、芽粗、根长、根数、根粗、球茎长、球茎宽进行统计。结果：两种试剂处理均能不同程度破除种子的休眠，提高萌发，其中氟啶酮10 mg/L，浸种24 h和钨酸钠588 mg/L，浸种12 h分别是两种试剂中的最优处理，主成分综合得分分别是1.58、0.12。结论：氟啶酮和钨酸钠处理多花黄精种子，能不同程度打破种子休眠限制，提高萌发率。

Abstract: Objective: To solve the problem of seed dormancy in the sexual propagation of Polygonatum cyrtonema seedlings, and to study the effects of different reagent treatments and soaking time on the germination of Polygonatum cyrtonema seeds. Methods: The collected Polygonatum cyrtonema seeds were treated with two reagents, sodium tungstate (588, 1176, 1764, 2352 mg/L) and fluridone (5, 10, 15, 20 mg/L), respectively, and the soaking time (12 h) was used, (18 h, 24 h) combined treatment, treated with sterile water as a control, after 70 d, the seed germination rate, germination potential, seed rot rate, and primary rhizome shoot length, shoot thickness, root length, root number, root thickness, bulb length, bulb width for statistics. Results: Both treatments could break the dormancy of seeds to different degrees and improve germination. Among them, fluridone 10 mg/L, seed soaking for 24 h and sodium tungstate 588 mg/L, seed soaking for 12 h were the optimal treatments, respectively. The composite scores of components were 1.58 and 0.12, respectively. Conclusion: Fluridone and sodium tungstate treatment of Polygonatum cyrtonema seeds can break the seed dormancy limit to different degrees and improve the germination rate.