一株菲降解菌的降解条件优化及生长动力学研究

Optimization of Degradation Conditions and Growth Kinetics of a Phenanthrene-Degrading Bacteria

摘要: 在实验室摇瓶规模下进行试验研究，确定一株属于假单胞菌属的菲降解菌的最适培养条件，最适pH为6.0，30℃，接种量10%，添加1000 mg/L丙酮酸钠作为共代谢底物可以达到最佳的降解效果，7 d可以将100 mg/L的菲降解92.76%。对于多种PAHs底物广谱性试验表明，7 d可以将30 mg/L的萘完全降解，对30 mg/L菲的降解率达93.53%，对30 mg/L芘的降解率为47.16%。本文的研究成果对于利用微生物修复实际污染土壤具有较好的参考意义。

Abstract: An experimental study was carried out on a laboratory shake flask scale to determine the optimum culture conditions for a phenanthrene-degrading bacteria belonging to the genus Pseudomonas. The optimum pH was 6.0, 30˚C, and the inoculation amount was 10%. The addition of 1000 mg/L sodium pyruvate as co-metabolism substrate could achieve the best degradation effect. And the bacteriacould degrade 100 mg/L of phenanthrene by 92.76% in 7d. For a variety of PAHs substrate broad-spectrum test showed that it can completely decompose 30 mg/L naphthalene, the degradation rate of phenanthrene to 30 mg/L was 93.53%, and the degradation rate to 30 mg/L technetium was 47.16% after 7 days. The research results in this paper have good reference significance for the use of microorganisms to remediate actual contaminated soil.