荧光法水相快速测定HRP酶浓度

Rapid Determination of HRP Enzyme Concentration in Aqueous Phase by Fluorescence Method

摘要: 本文采用2,6-二-(2-吡啶基)-4-吡啶甲酸作为水溶性的高荧光量子荧光探针，用于辣根过氧化物酶的检测。在pH = 7.2，25℃下，配体2,6-二-(2-吡啶基)-4-吡啶甲酸浓度为6 × 10−6 mol/L，H2O2浓度为1 × 10−4 mol/L时，回归方程为F = 60.934x + 1745.9，该体系的检测范围可达10−7~10−8 mol/L，灵敏度较高，操作简便，抗干扰能力强，可用作检测游离的辣根过氧化物酶和辣根过氧化物酶标记物的新方法，具有实际的应用前景。

Abstract: In this paper, 2,6-di-(2-pyridyl)-4-picolinic acid was used as a water-soluble high fluorescence quantum product as a fluorescence probe for the detection of HRP enzyme. At pH = 7.2 and 25˚C, the ligand PPA concentration is 6 × 10−6 mol/L, H2O2 concentration 1 × 10−4 mol/L, the regression equation is F = 60.934x + 1745.9. The detection range of the system can reach 10−7~10−8 mol/L. It has high sensitivity, simple operation and strong anti-interference ability. It can be used as a new method for the detection of free HRP and HRP markers, and has a practical application prospect.