mNGS在重症肺炎中的应用

Application of mNGS in Severe Pneumonia

摘要: 肺部感染是ICU中常见疾病，诊治过程中最重要的一环为确认肺部感染的病原微生物，针对该病原微生物对症使用抗生素，目前肺泡灌洗液传统微生物培养仍为确定病原微生物的金标准。重症肺炎患者病情变化快、病原微生物种类复杂，而传统微生物检测耗时长，对罕见微生物的检测有所欠缺，所以宏基因组二代测序技术(mNGS)逐渐应用于临床检测。mNGS运用于我科重症肺炎病原学检测后，发现了一些从前传统检测方法未检出的微生物，而这类病原体的及时、快速确定为治疗病情变化快的重症患者赢得了宝贵的时间。

Abstract: Pulmonary infection is a common disease in ICU. The most important part of the diagnosis and treatment process is to identify the pathogenic microorganisms of pulmonary infection, and antibi-otics are used for the pathogenic microorganisms. At present, the traditional microbial culture of alveolar lavage fluid is still the gold standard for identifying pathogenic microorganisms. The condi-tion of patients with severe pneumonia changes rapidly and the types of pathogenic microorgan-isms are complex. Traditional microbial detection takes a long time, and the detection of rare mi-croorganisms is lacking. Therefore, metagenomic next-generation sequencing (mNGS) technology is gradually applied in clinical detection. After mNGS was applied to the etiological detection of severe pneumonia in our department, some microorganisms that were not detected by traditional detec-tion methods were found, and the timely and rapid identification of such pathogens has won pre-cious time for the treatment of critically ill patients whose conditions change rapidly.