醋酸钠对氧糖剥夺损伤后PC12的影响
Effect of Sodiun Acetate on PC12 Injury after Oxygen-Glucose Deprivation
DOI: 10.12677/ACM.2022.12101386, PDF,    国家自然科学基金支持
作者: 张雅瑞：青岛大学基础医学院病理学与病理生理学，山东 青岛；侯庆明*：青岛大学基础医学院病理学与病理生理学，山东 青岛；青岛大学神经再生与康复研究院，山东 青岛
关键词: 氧糖剥夺；醋酸钠；ATF-6；神经保护；Oxygen-Glucose Deprivation； Sodium Acetate； ATF-6； Neuroprotection
摘要: 目的：通过OGD/R处理PC12细胞，证明NaAc在人类缺血性脑卒中中的作用和意义。方法：OGD模型的建立：用无氧糖培养液处理PC12细胞作为脑缺血再灌注损伤的体外模型；使用CCK-8比色法检测OGD损伤后NaAc对PC12细胞的保护状况；使用免疫印迹法Western blot检测OGD损伤后的NaAc对PC12中ATF-6蛋白水平的影响。结果：OGD损伤后，补充浓度为6 mM的NaAc可增加PC12的存活率(n = 6, F = 70.10, P < 0.05)；OGD损伤后，PC12中ATF-6的表达升高(t = 2.98, P < 0.05)；向正常的PC12细胞补充NaAc后ATF-6表达降低(t = 3.11, P < 0.05)；在损伤后的PC12细胞中添加浓度为6 mM的NaAc后，ATF-6的表达水平降低(n = 6, F = 2.09, P < 0.05)。结论：OGD损伤后，PC12中添加浓度为6 mM的NaAc是通过抑制ATF-6的表达来发挥神经保护作用。
Abstract: Objective: The purpose of investigating is demonstrated the effect and significance of NaAc in hu-man ischemic stroke by OGD/R treatment of PC12 cells. Methods: Establishment of OGD model: treatment of PC12 cells with Anaerobic sugar culture solution culture solution as an in vitro model of cerebral ischemia reperfusion injury; The protective status of NaAc on PC12 cells after OGD injury was detected using CCK-8 colorimetry; Effects of NaAc after OGD injury on ATF-6 protein levels in PC12 were detected using Western blotting methods. Results: After OGD injury, NaAc 6 mM sup-plementation increased PC12 survival (n = 6, F = 70.10, P < 0.05) and increased ATF-6 expression in PC12 (t = 2.98, P < 0.05). Supplementation of NaAc 6 mM to normal PC12 reduced the expression of ATF-6 (t = 3.11, P < 0.05). On the contrary, after OGD Supplementation of NaAc 6 mM to PC12 re-duced the expression of ATF-6 (n = 6, F = 20.09, P < 0.05). Conclusion: After OGD injury, intra-PC12 NaAc 6 mM supplementation exerts neuroprotective effects by inhibiting the expression of ATF-6 signaling pathway.
文章引用：张雅瑞, 侯庆明. 醋酸钠对氧糖剥夺损伤后PC12的影响[J]. 临床医学进展, 2022, 12(10): 9582-9588. https://doi.org/10.12677/ACM.2022.12101386

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